The orphan GPCR GPR87 was deorphanized and shown to be a lysophosphatidic acid receptor

Rho/ROCK pathway is essential to the expansion, differentiation, and morphological rearrangements of human neural stem/progenitor cells induced by lysophosphatidic acid

“…Similarly, an increase of ATX mRNA expression was observed in both noggin-treated cells and neurospheres. LPA 3 and LPA 5 mRNA were downregulated upon neural differentiation, and no signifi cant modulation was observed for LPA 2 and LPA 4 . Very low levels of sPLA 2 were observed at all stages of differentiation.…”

“…1 ). All of the undifferentiated and the differentiated hPSCs expressed LPA [1][2][3][4][5] , ATX , and sPLA 2 mRNA ( Fig. 1 ), with LPA 2 and…”

“…A negative control ( Ϫ RT) consisting in the absence of reverse transcriptase was performed to check the absence of genomic DNA. qPCR was carried out using TaqMan Universal master mix (Applied Biosystems) and the 7900HT Fast Real-Time PCR system (Applied Biosystems) and TaqMan gene expression assays for LPA [1][2][3][4][5] and ATX (Applied Biosystems). TaqMan gene expression assays for LPA 1 (Hs00173500_m1), LPA 2 (Hs00173704_m1), LPA 3 (Hs00173857_m1), LPA 4 (Hs00271072_s1), LPA 5 (Hs01051307_ m1), ATX (Hs00196470_m1), PLA2-group IIA (Hs00179898_ m1), ROCK1 (Hs01127699_m1), and ROCK2 (Hs00178154_m1) were used (Applied Biosystems).…”

“…1E, F ). Given the similar trend of expression of LPA [1][2][3][4][5] , ATX , and sPLA 2 mRNA in neurospheres from the two clones of iPSCs, we assessed most biological effects of LPA in iPS1 and compared them with hESCs. LPA 4 mRNA being the most abundant.…”

Expression patterns of the lysophospholipid receptor genes during mouse early development

“…Some are expressed at or near important local signaling centers such as the rostral forebrain, the ZLI, and the MHB, suggesting that lysophospholipid signaling might interact with well-documented developmental signaling pathways such as those of FGF, BMP, Wnt, and Shh in brain development. Recently, a number of additional LPA receptor genes belonging to the G protein-coupled P2Y receptor gene family have been identified, the functions of some of which have been suggested by comparison with human mutations (Tabata et al, 2007;Pasternack et al, 2008;Shimomura et al, 2008). It will be of considerable interest to explore the expression pattern and role of new genes such as Gpr87 and P2ry5 (P2y5).…”