The osmotic behavior and permeability to non-electrolytes of mitochondria

Tedeschi, Henry; Harris, Daniel L.

doi:10.1016/0003-9861(55)90092-8

Cited by 292 publications

(99 citation statements)

References 18 publications

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“…In contrast, glycerol (Table IV, exp 2, 3) and propylene glycol (exp 2 and 4) act as penetrants for both mitochondria and ghosts since the optical density approximates that of the suspension in the buffer. The results suggest that propylene glycol penetrates faster than glycerol (the OD at 30 s is higher), in complete agreement with previous studies with mitochondria suspensions (17,18).…”

Section: Resultssupporting

confidence: 92%

“…The corresponding reciprocal plots are shown in Fig. 1 13 (1/OD is directly proportional to volume in mitochondria and liposomes (18,19,12,1)). The experiment shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

“…A more indirect method, which has proven useful for both mitochondria (18,19) and liposomes (12,1), consists in measuring the light either transmitted or scattered by suspensions of the vesicles. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The permeability can be more accurately estimated by evaluating the rate of swelling when the particles are suspended in a penetrant (17,18). Although more precise permeability constants can be obtained by following the kinetics of the swelling (see e.g.…”

Section: Resultsmentioning

confidence: 99%

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Osmotic behavior and permeability of osmotically lysed mitochondria.

Bowman¹,

Tedeschi²,

DiDomenico³

et al. 1976

The Journal of Cell Biology

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Experiments were carried out with water-treated isolated rat liver mitochondria (mitochondrial ghosts) previously studied by Caplan and Greenawalt (Caplan, A.I., and J. W. Greenawalt. 1966. J. Cell Biol. 31:455-472) and Vasington and Greenawalt (Vasington, F., and J. Greenawalt. 1968. J. Cell Biol. 39:661-675).The ghosts have permeability properties and osmotic behavior comparable to those of isolated mitochondria. Although they have lost most of their internal contents, they must have resealed. Four properties were found which have not been previously described in systems derived from biological membranes: (a) an osmotic behavior in the virtual absence of internal components. (b) a selfarranging property in the formation of invaginations corresponding in morphology to the cristae. The results suggest that the assembly of the molecular components of the inner membrane is sufficient to specify the morphology. Hence the surface area to volume ratio of the vesicles may specify the presence or absence of cristaelike folds. (c) an increase in the permeability of the membranes to sucrose in the presence of iso-osmotic concentrations of sucrose. (d) an independence of the light transmitted by suspensions of the vesicles from the refractive index of the external medium. This observation runs counter to the general previous experience with either mitochondria or liposomes.

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Section: Resultssupporting

confidence: 92%

“…The corresponding reciprocal plots are shown in Fig. 1 13 (1/OD is directly proportional to volume in mitochondria and liposomes (18,19,12,1)). The experiment shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Osmotic behavior and permeability of osmotically lysed mitochondria.

Bowman¹,

Tedeschi²,

DiDomenico³

et al. 1976

The Journal of Cell Biology

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“…There are numerous indications from light scattering experiments (16,17) that mitochondrial swelling is multiphasic. In the scheme proposed, one of the observed phases would correspond to inner-membrane ssion.…”

Section: Inner Membrane Shape Changes: Evidence For Membrane Fusion Amentioning

confidence: 99%

Topology of the Mitochondrial Inner Membrane: Dynamics and Bioenergetic Implications

Mannella¹,

Pfeiffer²,

Bradshaw³

et al. 2001

IUBMB Life

220

167

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SummaryElectron tomography indicates that the mitochondrial inner membrane is not normally comprised of baf e-like folds as depicted in textbooks. In actuality, this membrane is pleomorphic, with narrow tubular regions connecting the internal compartments (cristae) to each other and to the membrane periphery. The membrane topologies observed in condensed (matrix contracted) and orthodox (matrix expanded) mitochondria cannot be interconverted by passive folding and unfolding. Instead, transitions between these morphological states likely involve membrane fusion and ssion. Formation of tubular junctions in the inner membrane appears to be energetically favored, because they form spontaneously in yeast mitochondria following large-amplitude swelling and recontraction. However, aberrant, unattached, vesicular cristae are also observed in these mitochondria, suggesting that formation of cristae junctions depends on factors (such as the distribution of key proteins and/or lipids) that are disrupted during extreme swelling. Computer modeling studies using the "Virtual Cell" program suggest that the shape of the inner membrane can in uence mitochondrial function. Simulations indicate that narrow cristae junctions restrict diffusion between intracristal and external compartments, causing depletion of ADP and decreased ATP output inside the cristae.

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Intracellular and Subcellular Oedema and Dehydration

Robin

Theodore

1976

Ciba Foundation Symposium 38 ‐ Lung Liquids

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The osmotic behavior and permeability to non-electrolytes of mitochondria

Cited by 292 publications

References 18 publications

Osmotic behavior and permeability of osmotically lysed mitochondria.

Osmotic behavior and permeability of osmotically lysed mitochondria.

Topology of the Mitochondrial Inner Membrane: Dynamics and Bioenergetic Implications

Intracellular and Subcellular Oedema and Dehydration

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