The oxidized phospholipids POVPC and PGPC inhibit growth and induce apoptosis in vascular smooth muscle cells

Fruhwirth, Gilbert O.; Moumtzi, Alexandra; Loidl, Alexandra; Ingoliç, Elisabeth; Hermetter, Albin

doi:10.1016/j.bbalip.2006.06.001

Cited by 55 publications

(48 citation statements)

References 34 publications

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“…This would suggest that the presence of serum phospholipases causes a breakdown of oxidized phospholipids into a product with anti-apoptotic effects. This is in partial agreement with a previous study; however, the anti-proliferative nature of the lipids was still seen with the oxidized phospholipid treatment in the presence of FCS [28]. PGPC rather than POVPC had the greater effect in all our experiments, despite the fact that the latter has been suggested to be the more potent of the two truncated oxidation products of PAPC [28,47].…”

Section: Discussionsupporting

confidence: 93%

“…This is in partial agreement with a previous study; however, the anti-proliferative nature of the lipids was still seen with the oxidized phospholipid treatment in the presence of FCS [28]. PGPC rather than POVPC had the greater effect in all our experiments, despite the fact that the latter has been suggested to be the more potent of the two truncated oxidation products of PAPC [28,47]. However, recently PGPC has been reported to have a higher toxicity in cultured macrophages due to more efficient membrane blebbing in apoptotic cells [49].…”

Section: Discussionsupporting

confidence: 93%

“…This correlates with the oxidized phospholipid data described in the present study, where VSMC death was induced after a short incubation time of only 2 h. Modified lipids could also be involved in the latter proliferative stages of vascular injury leading to the formation of neointima; however, this would be difficult to measure in vitro. Longer incubations would require medium containing growth supplements such as FCS which would lead to the breakdown of these lipids as shown in the present study and in a study by Fruhwirth et al [28].…”

Section: Discussionsupporting

confidence: 48%

“…PAPC also induces monocyte adhesion and inflammation in atherosclerotic mice in vivo [26]. Several studies, including some conducted in vivo, have demonstrated that oxidized phospholipids can influence many of the processes involved in vascular remodelling such as VSMC proliferation, migration and ECM production [27][28][29][30].…”

Section: Apoementioning

confidence: 99%

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Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation

et al. 2015

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The presence of inflammatory cells and MPO (myeloperoxidase) in the arterial wall after vascular injury could increase neointima formation by modification of phospholipids. The present study investigates how these phospholipids, in particular oxidized and chlorinated species, are altered within injured vessels and how they affect VSMC (vascular smooth muscle cell) remodelling processes. Vascular injury was induced in C57BL/6 mice and high fat-fed ApoE −/− (apolipoprotein E) mice by wire denudation and ligation of the left carotid artery (LCA). Neointimal and medial composition was assessed using immunohistochemistry and ESI-MS. Primary rabbit aortic SMCs (smooth muscle cells) were utilized to examine the effects of modified lipids on VSMC proliferation, viability and migration at a cellular level. Neointimal area, measured as intima-to-media ratio, was significantly larger in wire-injured ApoE −/− mice (3.62 + − 0.49 compared with 0.83 + − 0.25 in C57BL/6 mice, n = 3) and there was increased oxidized low-density lipoprotein (oxLDL) infiltration and elevated plasma MPO levels. Relative increases in lysophosphatidylcholines and unsaturated phosphatidylcholines (PCs) were also observed in wire-injured ApoE −/− carotid arteries. Chlorinated lipids had no effect on VSMC proliferation, viability or migration whereas chronic incubation with oxidized phospholipids stimulated proliferation in the presence of fetal calf serum [154.8 + − 14.2 % of viable cells at 1 μM PGPC (1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine) compared with control, n = 6]. In conclusion, ApoE −/− mice with an inflammatory phenotype develop more neointima in wire-injured arteries and accumulation of oxidized lipids in the vessel wall may propagate this effect.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 48%

Section: Apoementioning

confidence: 99%

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Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation

et al. 2015

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“…Oxidized phospholipids (OxPL) are implicated in various pathogeneses, because of which OxPL are considered cytotoxic [1][2][3] (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Mixing of Oxidized and Bilayer Phospholipids

Ranganathan

Singh

Ghafarian

2017

Biophysical Journal

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Composition and phase dependence of the mixing of 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), with the oxidized phospholipid, 1-palmitoyl-2-glutaryl-snglycero-3-phosphocholine (PGPC) were investigated by characterizing the aggregation states of DPPC/PGPC and DOPC/PGPC using a fluorescence quenching assay, dynamic light scattering, and time-resolved fluorescence quenching in the temperature range 5-60°C. PGPC forms 3.5 nm radii micelles of aggregation number 33. In the gel phase, DPPC and PGPC fuse to form mixed vesicles for PGPC molar fraction, X PGPC ≤ 0.3 and coexisting vesicles and micelles at higher X PGPC . Data suggest that liquid phase DPPC at 50°C forms mixed vesicles with segregated or hemi fused DPPC and PGPC for X PGPC ≤ 0.3. At 60°C, DPPC and PGPC do not mix, but form coexisting vesicles and micelles. DOPC and PGPC do not mix in any proportion in the liquid phase. Two dissimilar aggregates of the sizes of vesicles and PGPC micelles were observed for all X PGPC for T ≥ 22°C. DOPC-PGPC and DPPC-PGPC mixing is non-ideal for X PGPC N 0.3 in both gel and fluid phases resulting in exclusion of PGPC from the bilayer. Formation of mixed vesicles is favored in the gel phase but not in the liquid phase for X PGPC ≤ 0.3. For X PGPC ≤ 0.3, aggregation states change progressively from mixed vesicles in the gel phase to component segregated mixed vesicles in the liquid phase close to the chain melting transition temperature to separated coexisting vesicles and micelles at higher temperatures.

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Decoding chronic rhinosinusitis: A metabolomics‐based approach

Gong,

Fu,

Zhou

et al. 2024

Int Forum Allergy Rhinol

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BackgroundChronic rhinosinusitis (CRS) is a common and intractable disease in otorhinolaryngology, laying a heavy burden on healthcare systems. The worldwide researchers are making efforts to find solutions to this disease. Metabolomics has recently gained more and more traction, and might become a promising tool to unravel the complexity of CRS. This paper provides an overview of current studies on the metabolomics of various CRS subtypes.MethodsWe conducted a comprehensive literature search in PubMed, Web of Science, EMBASE, Google Scholar, and Cochrane Library, up to May 25, 2023. Search strategies incorporated key terms such as “chronic rhinosinusitis” and “metabolomics” with relevant synonyms and MeSH terms. Titles and abstracts of 86 screened articles were assessed for relevance to CRS and metabolomics. Methodological robustness, data reliability, and relevance were considered for shortlisted articles.ResultsAfter the refined process, a total of 26 articles were included in this study and sorted out by research themes, methodology and pivotal discoveries. These included studies identified the metabolic pathways and markers related to the pathophysiology in each subtype of CRS.ConclusionsMetabolomics helps to shed light on the complexity of CRS. The mentioned findings highlight the importance of specific metabolic pathways and markers in understanding the pathophysiology of CRS. Despite that, challenges and future directions in metabolomics research for CRS would be worth being further explored.

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The oxidized phospholipids POVPC and PGPC inhibit growth and induce apoptosis in vascular smooth muscle cells

Cited by 55 publications

References 34 publications

Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation

Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation

Mixing of Oxidized and Bilayer Phospholipids

Decoding chronic rhinosinusitis: A metabolomics‐based approach

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