The Pancreas and Known Factors of Acute Pancreatitis

Walkowska, Julia; Zielinska, Nicol; Karauda, Piotr; Tubbs, R. Shane; Kurtys, Konrad; Olewnik, Łukasz

doi:10.3390/jcm11195565

Cited by 21 publications

(19 citation statements)

References 108 publications

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“…One main function of NF-κB is the transcriptional regulation of the immune response. It plays a crucial role in the activation of leukocytes and is a central mediator of the innate and adaptive immune system. , In order to preliminary investigate the mechanism of action of MU, we conducted an exploration of the expression of IκBα and p65 dimer when the NF-κB pathway was activated. MU were tested for their ability to inhibit pancreatitis-associated NF-κB activation in LPS-induced RAW264.7 cell (Figure D–H).…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Nanoparticles Loaded with Ulinastatin for the Targeted Treatment of Acute Pancreatitis

et al. 2023

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Ulinastatin is commonly used in the clinic to treat acute pancreatitis (AP), but its therapeutic effect was limited by the presence of the blood−pancreas barrier (BPB) and low specificity. Here, we prepared a macrophage biomimetic nanoparticle (MU) that delivered ulinastatin to address the above issues. Macrophage membrane was used as a shell for a mixture of PEG−PLGA and ulinastatin. It was found that MU showed good stability and biocompatibility in vitro and in vivo. According to in vivo fluorescence imaging, MU displayed a great inflammation targeting effect both in a subcutaneous inflammation model and in situ pancreatitis mouse model, which was ascribed to the presence of adhesion proteins. In vitro and in vivo results demonstrated that MU have a superior AP treatment effect by inhibiting pro-inflammatory factors and keeping cells viability. It was suggested the MU could provide a new strategy for targeted AP treatment.

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Section: Resultsmentioning

confidence: 99%

Biomimetic Nanoparticles Loaded with Ulinastatin for the Targeted Treatment of Acute Pancreatitis

et al. 2023

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“…( b ) Viability of AR42J cells after culture with different concentrations of SPN. ( c ) Photographs of RBC haemolysis after incubation with various solutions for approximately 4 h: [ 1 ] H 2 O [ 2 ], NaCl, [ 3 ] DMEM [ 4 ], RPMI-1640 [ 5 ], PBS, and [ 6 ] CPPO@PFO. ( d ) Changes in animal body weight recorded over 30 days (n = 3).…”

Section: Resultsmentioning

confidence: 99%

“…Acute pancreatitis (AP) is one of the most common acute conditions affecting the abdomen [ 1 , 2 ]. The pathological basis of AP is the secretion of the enzymeogen prolyl protease, trypsinogen, and amylase from pancreatic acinar cells (PACs) to the pancreatic duct [ 3 ]. These enzymes are prematurely activated in PACs and self-digest in the pancreas.…”

Section: Introductionmentioning

confidence: 99%

A novel ROS-Related chemiluminescent semiconducting polymer nanoplatform for acute pancreatitis early diagnosis and severity assessment

Yin

Song

et al. 2023

J Nanobiotechnol

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Acute pancreatitis (AP) is a common and potentially life-threatening inflammatory disease of the pancreas. Reactive oxygen species (ROS) play a key role in the occurrence and development of AP. With increasing ROS levels, the degree of oxidative stress and the severity of AP increase. However, diagnosing AP still has many drawbacks, including difficulties with early diagnosis and undesirable sensitivity and accuracy. Herein, we synthesized a semiconducting polymer nanoplatform (SPN) that can emit ROS-correlated chemiluminescence (CL) signals. The CL intensity increased in solution after optimization of the SPN. The biosafety of the SPN was verified in vitro and in vivo. The mechanism and sensitivity of the SPN for AP early diagnosis and severity assessment were evaluated in three groups of mice using CL intensity, serum marker evaluations and hematoxylin and eosin staining assessments. The synthetic SPN can be sensitively combined with different concentrations of ROS to produce different degrees of high-intensity CL in vitro and in vivo. Notably, the SPN shows an excellent correlation between CL intensity and AP severity. This nanoplatform represents a superior method to assess the severity of AP accurately and sensitively according to ROS related chemiluminescence signals. This research overcomes the shortcomings of AP diagnosis in clinical practice and provides a novel method for the clinical diagnosis of pancreatitis in the future.

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“…Global fatty infiltration scores were calculated through the cumulative scoring of per lobular and intralobular fat severity. 7 Statistical Package for the Social Sciences (SPSS) software version 23 was employed for data analysis. Qualitative variables were expressed as frequencies and percentages.…”

Section: Methodsmentioning

confidence: 99%

Statin Induced Changes in Rat Pancreatic Tissue: A Histological and Biochemical Perspective

Qamar,

Khan,

Khan

et al. 2024

JRMC

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Objective: This study aimed to elucidate the effects of simvastatin on pancreatic histomorphology and biochemical profile in a rat model. Method: Forty male Sprague-Dawley rats weighing 250 g were divided into two equal groups: a control group (A) and an experimental group (B) treated with simvastatin 60 mg/kg/day for 12 weeks. At the end of the experiment, the rats were euthanized, and their pancreas was collected for analysis and examined using gold-standard histological parameters including inflammation and fatty infiltration. Biochemical parameters i.e. serum amylase and alkaline phosphatase were also analysed. Statistical Package for the Social Sciences (SPSS) software version 23 was employed for data analysis. Mean ± SD was employed to express quantitative variables. Qualitative variables were expressed as frequencies and percentages. Chi-square tests compared qualitative variables while independent sample t-tests compared quantitative variables between groups, with P < 0.05 defining statistical significance. Results: The administration of Simvastatin resulted in a substantial induction of pancreatic inflammation and fatty infiltration, accompanied by notable alterations in the biochemical profile. Specifically, there was a significant reduction in serum amylase levels and a concomitant increase in serum alkaline phosphatase (ALP) levels, as compared to the control group. Conclusion: This study demonstrates that simvastatin profoundly alters pancreatic histomorphology in rats, resulting in marked inflammation, necrosis, fatty infiltration, and interstitial fibrosis. These novel findings provide unique insights into simvastatin's effects on pancreatic morphology using a robust animal model. Keywords: Simvastatin; Sprague-Dawley rats; pancreatic histomorphology, Amylase, Alkaline phosphatase.

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The Pancreas and Known Factors of Acute Pancreatitis

Cited by 21 publications

References 108 publications

Biomimetic Nanoparticles Loaded with Ulinastatin for the Targeted Treatment of Acute Pancreatitis

Biomimetic Nanoparticles Loaded with Ulinastatin for the Targeted Treatment of Acute Pancreatitis

A novel ROS-Related chemiluminescent semiconducting polymer nanoplatform for acute pancreatitis early diagnosis and severity assessment

Statin Induced Changes in Rat Pancreatic Tissue: A Histological and Biochemical Perspective

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