The PapG protein is the alpha-D-galactopyranosyl-(1----4)-beta-D-galactopyranose-binding adhesin of uropathogenic Escherichia coli.

Abstract

Uropathogenic Escherichia coli adhere to uroepithelial cells by their digalactoside a-D-galactopyranosyl-

Protein secretion and the pathogenesis of bacterial infections

“…Employing adhesion and retraction properties of pili, bacteria advance against the flow of urine and colonize the lower (cystitis) or upper (pyelonephritis) urinary tract of humans. E. coli strains associated with pyelonephritis display Pap pili, structures that are composed of three parts: (1) the tip adhesin; (2) the fibrillum; and (3) the pilus rod (Lindberg et al 1987;Kuehn et al 1992). The base of the pilus rod is embedded in the outer membrane of Gramnegative bacteria.…”

“…Pap (type I) pili are assembled from six pilins, all of which are encoded by a single large operon (Soto and Hultgren 1999). To identify the position of pilins within the pilus, wild-type E. coli and mutants lacking single pilin genes were analyzed by electron microscopy and immuno-gold labeling (Norgren et al 1984;Baga et al 1987;Lindberg et al 1987;Kuehn et al 1992;Jacob-Dubuisson et al 1993). PapG pilin (G in Fig.…”

Profile of Scott J. Hultgren

“…Normark had discovered that the so-called P pilus, produced by strains of E. coli that cause kidney infections, contained a specialized adhesive protein known as the PapG adhesin that mediates binding of the bacteria to host cells (4). Through his doctoral research, Hultgren had become interested in studying the molecular basis of pili formation and function, so he applied to become a postdoctoral fellow in Normark's laboratory, then located at the University of Umeå in Umeå, Sweden, where Hultgren's son, Nils, was soon born.…”

Role of intestinal surfactant-like particles as a potential reservoir of uropathogenic Escherichia coli

“…P pili [2,27] and the PapD^PapG complex [5,28] were isolated from E. coli strain HB101 bearing the appropriate plasmid as described previously. The papG gene was subcloned from plasmid pPAP5 that contains the entire pap operon cloned from the E. coli strain J96 isolate of serotype O4:K6 [29], derived from a case of human pyelonephritis.…”