The past, present and future of nongonadal LH/hCG actions in reproductive biology and medicine

Rao, Ch.V.; Lei, Zhenmin

doi:10.1016/j.mce.2006.07.007

Cited by 72 publications

(73 citation statements)

References 92 publications

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“…In the placenta, hCG is well known to facilitate trophoblastic differentiation (Licht et al, 2001, Malassine & Cronier, 2002, and was reported to induce the expression of specific genes such as VEGF, leukemia inhibitory factor, and MMP-9, all central to the establishment of the feto-maternal interface (Licht et al, 2007, Zygmunt et al, 2002. In human placenta, hCG is primarily produced by the syncytiotrophoblast and to a certain extent by the cytotrophoblast (Kirkegaard et al, 2011, Krantz et al, 2004, Rao & Lei, 2007, Shi et al, 1993. In normal pregnancies, detectable levels of hCG begin to appear in the maternal circulation about 2-3 weeks after conception and the peak is observed at ~8-9 wg before declining significantly in the later stages of pregnancy.…”

Section: Regulation By Human Chorionic Gonadotropin (Hcg)mentioning

confidence: 99%

Role of EG-VEGF in Human Placentation: Physiological and Pathological Implications

Hoffmann¹,

Brouillet²,

Benharouga³

et al. 2012

Recent Advances in Research on the Human Placenta

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Section: Regulation By Human Chorionic Gonadotropin (Hcg)mentioning

confidence: 99%

Role of EG-VEGF in Human Placentation: Physiological and Pathological Implications

Hoffmann¹,

Brouillet²,

Benharouga³

et al. 2012

Recent Advances in Research on the Human Placenta

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“…Studies performed over the past decade indicate that LHCGR is expressed in the human endometrium (Licht et al 2003;Rao and Lei 2007); however, these findings are in contrast with those reported by Stewart et al (1999). Zhang et al (2001b) reported finding Lhcgr transcripts in the mouse reproductive tract.…”

Section: Discussionmentioning

confidence: 68%

“…According to our previous observations and those of other groups, hCG plays important paracrine roles in implantation, angiogenesis and immune tolerance (Perrier d'Hauterive et al 2004Edwards 2006;Licht et al 2007;Rao and Lei 2007). Because of Fig.…”

Section: Discussionmentioning

confidence: 76%

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Gridelet

Tsampalas

Berndt

et al. 2013

Reprod. Fertil. Dev.

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Abstract. The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.

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“…Previous studies on LHR in cattle (Sun et al, 1997;Shemesh et al 2001), pig (Ziecik, 1986;Gawronska, 1999;Wasowicz and Ziecik, 2000), goat (Shen et al, 2009), mouse (Zhang et al, 2001), rabbit, and rat (Sawitzke and Odell, 1991 ) showed that LHR is expressed in a wide variety of non-gonadal tissues and organs, such as oviduct, cervix, urinary bladder, placenta, fetal membranes, umbilical cord, brain, pineal gland, spinal cord, pituitary gland, breast, skin, adrenal gland, blood vessels in target tissues, cells of the immune system, bone, heart, stomach, spleen, kidney, muscle, liver, and intestine (Rao and Lei, 2007;Ziecik et al, 2007;Vischer and Bogerd, 2003;Kwok et al, 2005;Chen et al, 2010). In the present study, LHR mRNA and protein were detected in non-gonadal tissues from Hu sheep using real-time quantitative PCR and ELISA.…”

Section: Discussionmentioning

confidence: 99%

Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle

Wang

Zhang²,

Gao³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR). In order to examine the expression patterns of the LHR gene in non-gonadal tissues during the 4 phases of the ovine estrous cycle, tissues from healthy non-pregnant adult Hu sheep were examined according to the estrous cycle for normal ovaries using real-time fluorescence quantitative PCR and ELISA methods with GAPDH as the reference gene. LHR mRNA expression levels were significantly correlated with protein concentrations and the LHR gene was abundantly expressed in olfactory bulb, hypothalamus, rumen, small intestine, kidney, and uterine tissues. When comparing the expression levels of LHR during the 4 estrous phases in particular tissues, the results showed that LHR expression levels were significantly different and relatively lower at the estrous stage in a number of non-gonadal tissues. The trends of change in LHR expression levels were highly significantly correlated between hypothalamus and rectum, hypophysis and oviduct, ileum and 3767 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 3766-3780 (2012) Expression of the LHR gene in ovine non-gonadal tissues uterus, and among jejunum, olfactory bulb, and kidney (P < 0.01), and there was also significant correlation between duodenum and oviduct, hypothalamus and medulla oblongata, jejunum and uterus, omasum and abomasum, and reticulum and colon (P < 0.05). These results indicate that the ovine LHR gene (or LH) might control important mechanisms in nongonadal tissues and that the level of LH activity in some tissues may be influenced by hormonal status during the estrous cycle.

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The past, present and future of nongonadal LH/hCG actions in reproductive biology and medicine

Cited by 72 publications

References 92 publications

Role of EG-VEGF in Human Placentation: Physiological and Pathological Implications

Role of EG-VEGF in Human Placentation: Physiological and Pathological Implications

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle

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