The phase variation between wrinkly and smooth colony phenotype affects the virulence of Vibrio parahaemolyticus

Wu, Qimin; Li, Xue; Zhang, Miaomiao; Xue, Xingfan; Zhang, Tingting; Sun, Hui; Xiong, Shuhui; Lu, Renfei; Zhang, Yiquan; Zhou, Min

doi:10.1007/s00203-023-03719-1

Cited by 1 publication

(1 citation statement)

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“…The cps but not scv gene cluster is required for the wrinkly colony formation, as only the cps gene mutants form smooth colonies on the plate (Liu et al, 2022). V. parahaemolyticus undergoes the wrinkly and smooth phase variation, which affects biofilm formation and virulence factor production (Wu et al, 2022(Wu et al, , 2023.…”

Section: Introductionmentioning

confidence: 99%

The effect of environmental calcium on gene expression, biofilm formation and virulence of Vibrio parahaemolyticus

Li,

Chang,

Zhang

et al. 2024

Front. Microbiol.

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Calcium (Ca2+) can regulate the swarming motility and virulence of Vibrio parahaemolyticus BB22. However, the effects of Ca2+ on the physiology of V. parahaemolyticus RIMD2210633, whose genomic composition is quite different with that of BB22, have not been investigated. In this study, the results of phenotypic assays showed that the biofilm formation, c-di-GMP production, swimming motility, zebrafish survival rate, cytoxicity against HeLa cells, and adherence activity to HeLa cells of V. parahaemolyticus RIMD2210633 were significantly enhanced by Ca2+. However, Ca2+ had no effect on the growth, swarming motility, capsular polysaccharide (CPS) phase variation and hemolytic activity. The RNA sequencing (RNA-seq) assay disclosed 459 significantly differentially expressed genes (DEGs) in response to Ca2+, including biofilm formation-associated genes and those encode virulence factors and putative regulators. DEGs involved in polar flagellum and T3SS1 were upregulated, whereas majority of those involved in regulatory functions and c-di-GMP metabolism were downregulated. The work helps us understand how Ca2+ affects the behavior and gene expression of V. parahaemolyticus RIMD2210633.

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Section: Introductionmentioning

confidence: 99%