The phenotypic and molecular characteristics of antimicrobial resistance of Salmonella enterica subsp. enterica serovar Typhimurium in Henan Province, China

Dong, Ning; Li, Yongrui; Zhao, Jingbo; Ma, Hui; Wang, Jinyan; Liang, Beibei; Du, Xinying; Wu, Fuli; Xia, Shengli; Yang, Xiaoxia; Liu, Hongbo; Yang, Chaojie; Qiu, Shaofu; Song, Hongbin; Jia, Leili; Li, Yan; Sun, Yansong

doi:10.1186/s12879-020-05203-3

Cited by 28 publications

(23 citation statements)

References 51 publications

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“…Kentucky, and S . Typhimurium strains but not in other serotypes ( 7 , 25 – 27 ). Instead, PMQR genes, often in the form of multiple PMQR genes located in the chromosome and plasmids, now are commonly detectable in ciprofloxacin-resistant Salmonella strains of various serotypes.…”

Section: Discussionmentioning

confidence: 88%

“…Currently, target gene mutations, in particular single mutation in the gyrA gene, may be detected in S. Typhimurium but is rarely seen in other serotypes of Salmonella. Double mutations in gyrA and single mutation in parC that mediated high-level resistance were seen only in S. Indiana, S. Kentucky, and S. Typhimurium strains but not in other serotypes (7,(25)(26)(27). Instead, PMQR genes, often in the form of multiple PMQR genes located in the chromosome and plasmids, now are commonly detectable in ciprofloxacinresistant Salmonella strains of various serotypes.…”

Section: Discussionmentioning

confidence: 99%

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Evolution of Ciprofloxacin Resistance-Encoding Genetic Elements in Salmonella

et al. 2020

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Evolution of Ciprofloxacin Resistance-Encoding Genetic Elements in Salmonella

et al. 2020

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“…ST19 and ST34 prevalence in Salmonella 4,[5],12:i:- has significantly increased in Canada, and some of these STs demonstrate quinolone resistance [ 30 ]. It has been reported that ST19 is significantly associated with ciprofloxacin resistance in China [ 31 ] and that ST34 is linked to the nalidixic acid resistance in Africa [ 32 ]. Salmonella Agona ST13 was the most prevalent serovar isolated from chicken meat in Sri Lanka [ 33 ], and a Salmonella Agona strain isolated from chicken meat in China possessed a T57S substitution in ParC and carried qnrS [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

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Non-typhoidal salmonellosis remains a pressing public health problem worldwide. Quinolones, particularly fluoroquinolones, are widely used to treat various infections, including non-typhoidal salmonellosis, which can be a serious illness. The emergence of fluoroquinolone-resistant Salmonella has resulted in treatment failure and high mortality rates. In this study, we estimated the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea from 2016 to 2019. We evaluated the association of these genes with fluoroquinolone susceptibility. Antimicrobial susceptibility tests for Salmonella isolates were performed using the Vitek II system, and the minimum inhibitory concentrations (MIC) of ciprofloxacin and levofloxacin were determined using the E-test method. Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. Among them, 22 Salmonella strains harbored one PMQR gene (qnrA, qnrB, or qnrS), and three Salmonella strains carried two PMQR genes (qnrS and aac(6′)-Ib-cr or qnrA and qnrB). qnrS was the most common PMQR gene. Serotyping revealed that Salmonella 4,[5]12:i:- (32.4%, 11/34) and Salmonella Typhimurium (29.4%, 10/34) were the two most predominant serovars, and Multi-locus sequence typing (MLST) showed that ST19 and ST34 were the most frequent sequence types. In conclusion, qnr gene-positive Salmonella 4,[5],12:i:- and Salmonella Typhimurium were the main serovars responsible for reduced susceptibility to fluoroquinolones. Therefore, our findings suggest that PMQR-positive Salmonella strains, which can be isolated from various samples including human, food, and the environment, should be carefully monitored.

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“…Although S. Enteritidis usually causes predominantly self-limiting enteric disease, some severe clinical illnesses require antibiotics. In particular, fluoroquinolones and extended-spectrum cephalosporins, such as cefotaxime and ceftazidime, are considered first-line clinical drugs [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and Characterization of CTX-M-55-Type Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serovar Enteritidis Isolated from Patients in Shanghai, China

et al. 2021

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The emergence of extended-spectrum β-lactamase-producing Salmonella enterica serovar Enteritidis (ESBL-SE) in humans and foods has gained global attention. In particular, CTX-M-type ESBL-SE are increasingly being detected from various sample types. The aim of this study was to comprehensively analyze the epidemiology and characteristics of blaCTX-M-55-carrying ESBL-SE isolates of clinical origin in Shanghai, China. A total of 292 S. Enteritidis isolates were recovered from the feces and blood of outpatients and inpatients between 2006 and 2014. Overall, there was a high frequency of cefotaxime-resistant isolates (97.3%), which was significantly higher (p < 0.01) than that of isolates resistant to the other tested antibiotics. All S. Enteritidis isolates exhibited resistance to ≥1 antibiotic, and 98.0% were multidrug resistant. A total of 233 isolates were identified as ESBL-SE, 166 of which were CTX-M type. Six subtypes of CTX-M-encoding genes were detected, among which blaCTX-M-55 (91.6%, 152/166) was the most prevalent genotype. There was high genetic similarity among blaCTX-M-55-positive ESBL-SE. The blaCTX-M-55 gene in the ESBL-SE donor strains could be easily transferred into Enterobacteriaceae recipient strains. This study highlights that CTX-M-55 should be considered an important surveillance target in Shanghai, China. Cephalosporins, especially cefotaxime, must be used with caution in empirical treatment for Salmonella infections.

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The phenotypic and molecular characteristics of antimicrobial resistance of Salmonella enterica subsp. enterica serovar Typhimurium in Henan Province, China

Cited by 28 publications

References 51 publications

Evolution of Ciprofloxacin Resistance-Encoding Genetic Elements in Salmonella

Evolution of Ciprofloxacin Resistance-Encoding Genetic Elements in Salmonella

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Epidemiology and Characterization of CTX-M-55-Type Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serovar Enteritidis Isolated from Patients in Shanghai, China

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