The PhoP/Q two-component regulatory system is essential for Xylella fastidiosa survival in Vitis vinifera grapevines

Pierce, Brittany; Kirkpatrick, Bruce C.

doi:10.1016/j.pmpp.2014.12.003

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…No PD symptom was observed in the grapevines inoculated with popP deletion strain in compared with severe PD symptom developed by grapevines infected with wild type X. fastidiosa and complementary strain XfΔpopP-C consistently observed in repeated greenhouse inoculation tests. Similarity results were recently reported on the pathogenicity of Xf popP [37]. Our results demonstrate that popP is an important virulence factors and plays critical role for the survivals and the pathogenicity of X. fastidiosa.…”

Section: Discussionsupporting

confidence: 91%

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Lin¹

2015

J Plant Pathol Microbiol

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Xylella fastidiosa (Xf) possess a two component regulatory system (TCS) PopP-PopQ which differentially regulates genes in response to environmental stimuli. To elucidate the role of popP in the pathogenicity of X. fastidiosa causing Pierce's disease (PD) of grapevine, a site-directed deletion method and chromosome-based genetic complementation strategy were employed to create popP deletion mutant XfΔpopP and its complementary strain XfΔpopP-C. In vitro studies showed that while all strains had similar growth curves, XfΔpopP showed significant reduction in cell-cell aggregation and cell-matrix adherence. Biofilm production of XfΔpopP was about 42% less than that of wild type X. fastidiosa and XfΔpopP-C. No symptoms were observed in grapevines inoculated with XfΔpopP, whereas grapevines inoculated with wild type X. fastidiosa and XfΔpopP-C showed typical PD symptoms. Several biofilm-related genes and genes involving protein secretary systems were down regulated in XfΔpopP in compared with wild type X. fastidiosa and XfΔpopP-C. These in vitro and in planta assay results provide strong evidence that the role of PopP is required for pathogenicity of X. fastidiosa on grapevine.

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Section: Discussionsupporting

confidence: 91%

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Lin¹

2015

J Plant Pathol Microbiol

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“…Interestingly, genes involved in production (rpfF) and sensing (rpfC and rpfG) of the quorumsensing molecule diffusible signal factor (DSF), which is critical for acquisition of X. fastidiosa by insects from plants [62], and regulates plant host specificity [69], were detected in high frequencies. Other regulatory genes detected here, have been shown to be important for virulence of X. fastidiosa; interestingly, phoP/phoQ [70] were shown to be needed for survival in plants.…”

Section: Recombinant Regions Shared Among Subspecies Inform Eco-evolumentioning

confidence: 67%

Patterns of inter- and intrasubspecific homologous recombination inform eco-evolutionary dynamics of Xylella fastidiosa

et al. 2019

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High rates of homologous recombination (HR) in the bacterial plant pathogen Xylella fastidiosa have been previously detected. This study aimed to determine the extent and explore the ecological significance of HR in the genomes of recombinants experimentally generated by natural transformation and wild-type isolates. Both sets of strains displayed widespread HR and similar average size of recombined fragments consisting of random events (2-10 kb) of inter-and intrasubspecific recombination. A significantly higher proportion and greater lengths (>10 kb, maximum 31.5 kb) of recombined fragments were observed in subsp. morus and in strains isolated in Europe from intercepted coffee plants shipped from the Americas. Such highly recombinant strains pose a serious risk of emergence of novel variants, as genetically distinct and formerly geographically isolated genotypes are brought in close proximity by global trade. Recently recombined regions in wild-type strains included genes involved in regulation and signaling, host colonization, nutrient acquisition, and host evasion, all fundamental traits for X. fastidiosa ecology. Identification of four recombinant loci shared between wild-type and experimentally generated recombinants suggests potential hotspots of recombination in this naturally competent pathogen. These findings provide insights into evolutionary forces possibly affecting the adaptive potential to colonize the host environments of X. fastidiosa.

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“…This system can respond to the relatively harsh environment of the xylem, particularly to acidic pH, by inducing adaptive changes and protective phenotypes in the pathogen. These changes include the formation of cell aggregates that are better enabled to cope with environmental stress [49].…”

Section: Bacterial Action In the Xylemmentioning

confidence: 99%

Xylella fastidiosa: A Glimpse of the Portuguese Situation

Loureiro,

Mesquita,

Dapkevicius

et al. 2023

Microbiology Research

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Xylella fastidiosa (Xf) is classified as a quarantine pest due to its consequences on economically significant crops. Its main form of transmission in Europe is through the insect Philaenus spumarius. Due to climate change, the populations of insect vectors have become more extensive, resulting in the dissemination of the bacteria over longer periods, but the destruction of these insects raises issues due to their role in nature. Upon infection, Xf causes the occlusion of xylem vessels via bacterial aggregates and tylosis production by the plant as a response to infection. Although symptomatic manifestations of Xf are often linked to water stress, a variety of plant species have been found to carry the pathogen without symptoms, making it all too easy to evade detection when relying on visual inspections. Beyond water stress, other conditions (individual plant resistance/tolerance, bacterial concentrations, transpiration rates, and interactions between subspecies) may be implicated in symptom development. A thorough understanding of how this disease develops, especially its capacity to spread from the initial focus and establish a systemic infection, is imperative. This review focuses on the Xf infection process, the development of symptoms, its spread within Portugal, and the actions that have been taken to counter it.

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The PhoP/Q two-component regulatory system is essential for Xylella fastidiosa survival in Vitis vinifera grapevines

Cited by 10 publications

References 36 publications

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Patterns of inter- and intrasubspecific homologous recombination inform eco-evolutionary dynamics of Xylella fastidiosa

Xylella fastidiosa: A Glimpse of the Portuguese Situation

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