The photodynamic and direct actions of methylene blue on mitochondrial energy metabolism: A balance of the useful and harmful effects of this photosensitizer

Klosowski, Eduardo Makiyama; Souza, Byanca Thais Lima de; Mito, Márcio Shigueaki; Constantin, Renato Polimeni; Mantovanelli, Gislaine Cristiane; Mewes, Juliana Morais; Bizerra, Paulo Francisco Veiga; Menezes, Paulo Vinicius Moreira da Costa; Gilglioni, Eduardo Hideo; Utsunomiya, Karina Sayuri; Marchiosi, Rogério; Santos, Wanderley Dantas dos; Ferrarese-Filho, Osvaldo; Caetano, Wilker; Pereira, Paulo Cesar de Souza; Gonçalves, Renato Sonchini; Constantin, Jorgete; Ishii–Iwamoto, Emy Luiza; Constantin, Rodrigo Polimeni

doi:10.1016/j.freeradbiomed.2020.04.015

Cited by 30 publications

(20 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be noted that the use of MB as a drug against tumors requires a drug activation step by illumination at an appropriate wavelength (≈640 nm). 47 Upon exposure to light, the photosensitizer (MB molecules) would start generating reactive oxygen species (ROS), eventually inducing cell death. In the experiments discussed so far, MB was applied without light activation (dark conditions) so that no significant cytotoxic activity was observed ( Figure S12 ).…”

Section: Results and Discussionmentioning

confidence: 99%

“…We finally sought to monitor the SERS signal at different MB concentrations over time, correlating with the cell viability resulting from treatments with the selected MB doses. It should be noted that the use of MB as a drug against tumors requires a drug activation step by illumination at an appropriate wavelength (≈640 nm) . Upon exposure to light, the photosensitizer (MB molecules) would start generating reactive oxygen species (ROS), eventually inducing cell death.…”

Section: Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nanocomposite Scaffolds for Monitoring of Drug Diffusion in Three-Dimensional Cell Environments by Surface-Enhanced Raman Spectroscopy

et al. 2021

View full text Add to dashboard Cite

Monitoring dynamic processes in complex cellular environments requires the integration of uniformly distributed detectors within such three-dimensional (3D) networks, to an extent that the sensor could provide real-time information on nearby perturbations in a non-invasive manner. In this context, the development of 3D-printed structures that can function as both sensors and cell culture platforms emerges as a promising strategy, not only for mimicking a specific cell niche but also toward identifying its characteristic physicochemical conditions, such as concentration gradients. We present herein a 3D cancer model that incorporates a hydrogel-based scaffold containing gold nanorods. In addition to sustaining cell growth, the printed nanocomposite inks display the ability to uncover drug diffusion profiles by surface-enhanced Raman scattering, with high spatiotemporal resolution. We additionally demonstrate that the acquired information could pave the way to designing novel strategies for drug discovery in cancer therapy, through correlation of drug diffusion with cell death.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Nanocomposite Scaffolds for Monitoring of Drug Diffusion in Three-Dimensional Cell Environments by Surface-Enhanced Raman Spectroscopy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several types of PSs, such as phenothiazine dyes (methylene blue ( Klosowski et al, 2020 ), toluidine blue O ( Moslemi et al, 2018 )), phthalocyanine ( Liang et al, 2021 ), porphyrin ( Mala et al, 2021 ) have been proved to have significant inactivation effects. In recent years, consumers are more enthusiastic about natural products, therefor natural additives are very attractive to food manufacturers.…”

Section: Factors Influencing Pdimentioning

confidence: 99%

The application of photodynamic inactivation to microorganisms in food

et al. 2021

View full text Add to dashboard Cite

“…MB concentration is found to be higher in the brain than in plasma after oral administration or intravenous injection in rats [ 30 ]. Besides, it has a strong affinity for mitochondria [ 31 ]. Different from the other antioxidants such as MitoQ and MitoVitE, MB can reduce the production of free radicals by bypassing Complex I/III activity rather than scavenging free radicals [ 32 ].…”

Section: Applicationsmentioning

confidence: 99%

The Potentials of Methylene Blue as an Anti-Aging Drug

Xue

Thaivalappil

Cao

2021

Cells

View full text Add to dashboard Cite

Methylene blue (MB), as the first fully man-made medicine, has a wide range of clinical applications. Apart from its well-known applications in surgical staining, malaria, and methemoglobinemia, the anti-oxidative properties of MB recently brought new attention to this century-old drug. Mitochondrial dysfunction has been observed in systematic aging that affects many different tissues, including the brain and skin. This leads to increaseding oxidative stress and results in downstream phenotypes under age-related conditions. MB can bypass Complex I/III activity in mitochondria and diminish oxidative stress to some degree. This review summarizes the recent studies on the applications of MB in treating age-related conditions, including neurodegeneration, memory loss, skin aging, and a premature aging disease, progeria.

show abstract

The photodynamic and direct actions of methylene blue on mitochondrial energy metabolism: A balance of the useful and harmful effects of this photosensitizer

Cited by 30 publications

References 95 publications

Nanocomposite Scaffolds for Monitoring of Drug Diffusion in Three-Dimensional Cell Environments by Surface-Enhanced Raman Spectroscopy

Nanocomposite Scaffolds for Monitoring of Drug Diffusion in Three-Dimensional Cell Environments by Surface-Enhanced Raman Spectroscopy

The application of photodynamic inactivation to microorganisms in food

The Potentials of Methylene Blue as an Anti-Aging Drug

Contact Info

Product

Resources

About