The Physiological Response of Prostatic and Vesicular Transplants in the Anterior Chamber of the Eye

Abstract

Markee (1) with Schochet (2) first used transplants of tissue in the eye for physiological observations. With transplants of endometrium in the rabbit, they observed the color changes in the estral cycle and the relation of the vascular changes to muscular contractions (3). Later, Markee and Andersen (4) devised a method for measurement of the size of the transplants by camera lucida drawings and observed a definite series of changes during pregnancy. Litt (5) placed placental tissue in the eyes of pregnant rabbits and observed no effect on the subsequent lactation. After 30 days of growth and establishment of vascularity, there was gradual degeneration and absorption. Goodman (6) with homoiotransplants of immature ovaries in intact and spayed male and female rats observed a number of physiological phenomena. A successful take in a spayed female was accompanied as a rule by regular estral cycles. In the male rat, follicles were formed but corpora lutea did not appear unless extracts of pregnancy urine were administered. The administration of the female sex hormone in the male and female was followed by complete or partial atrophy of the ovarian grafts.From this incomplete review of the literature, it is clear that, at least with certain organs, transplants in the eye are functioning viable tissues and may be used for physiological observations. With the photographic method which has been described in the preceding paper, it has been possible to follow accurately changes in the size of seminal vesicular and prostatic transplants under the influence of parenterally administered hormones in intact and castrated male rabbits. MetkodsThe methods of transplantation and observations of size were identical with the procedure outlined in the preceding paper (7). The hormones used were those 281

The Relation of Blood Cholesterol and the Size of Prostatic and Vesicular Transplants in the Anterior Chamber of the Eye

“…The procedures described in the preceding papers (6,7) were used for the transplantation and measurement of the grafts in the eyes of male rabbits. Free cholesterol was determined according to the blood lipid methods of Kirk, Page and Van Slyke (8).…”

“…In view of the conspicuous increase in the size of prostatic and vesicular transplants following injections of estrin in the male rabbit reported in the preceding papers (6,7), it seemed desirable to investigate the level of the blood cholesterol during the period of the enlargement. Since other hormones also caused enlargement, it was of interest to study these two factors under the influence of the male sex hormone and the anterior pituitary-like substance of pregnancy urine.…”

Intraocular in vivo imaging of pancreatic islet cell physiology/pathology

“…pieces of skin) into the ACE of dogs and rabbits in 1873 [8] . Since then, the ACE has been used as a transplantation site for a variety of tissues ranging from ovaries [9] , prostatic tissue [10] , peripheral and central nervous tissues [11] , [12] , [13] , kidney glomeruli [14] , and pancreatic tissue [15] , [16] , [17] , [18] . With this information at hand, we hypothesized that the ACE should represent a unique transplantation site for pancreatic islets utilizing the cornea as a natural body-window for functional in vivo imaging of islet cells.…”

Nouvelles recherches concernant l’action d’extraits prostatiques sur l’autogreffe de prostate dans la chambre antérieure de l’œil du lapin. (Avec Planche I)

“…Un grand nombre de tissus normaux ont été greffés également avec succès (autogreffes ou greffes homéoplastiques) (voir à ce sujet D a m er o n ) et l'on a pu étudier de cette façon leur métabolisme. Pour la prostate, par exemple, qui nous intéresse ici, on a pu démontrer d'une part une augmentation de volume du greffon sous l'influence d'extraits du lobe antérieur de l'hypophyse (9) et d'autre part une diminution sous l'influence de la castration (15,16). Dans une première série Bianchi/Roulet/Cuendet/Grambcrg VII/1958 d'expériences, nous avons pu mettre en évidence l'action activante qu'un extrait de prostate exerce sur des autogreffes de cette glande (2) ; nous avons poursuivi ces recherches en augmentant d'une part le nombre de greffes et d'autre part le nombre des contrôles et des témoins.…”