The phytochrome interacting proteins ERF55 and ERF58 repress light-induced seed germination in Arabidopsis thaliana

Li, Zenglin; Sheerin, David J.; Roepenack‐Lahaye, Edda von; Stahl, Mark; Hiltbrunner, Andreas

doi:10.1038/s41467-022-29315-3

Cited by 30 publications

(20 citation statements)

References 51 publications

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“…The TFs are considered as ideal candidates for crop improvement because their overexpression enhances tolerances to multiple abiotic and biotic stresses in transgenic plants [ 25 ]. Extensive studies have demonstrated that AP2/ERF TFs are crosstalk factors in stress signal pathways involved in salicylic acid, jasmonic acid, ethylene, and abscisic acid signal transduction pathways [ 26 ], and hence play vital roles in regulating plant growth and development as well as in response to diverse stresses [ 27 , 28 ]. Although AP2/ERF TFs have been thoroughly identified and characterized in many plant species, very little is known about the AP2/ERF gene family in ramie.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of AP2/ERF Gene Superfamily in Ramie (Boehmeria nivea L.) Revealed Their Synergistic Roles in Regulating Abiotic Stress Resistance and Ramet Development

Qiu

Zhao

Abubakar

et al. 2022

IJMS

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AP2/ERF transcription factors (TFs) are one of the largest superfamilies in plants, and play vital roles in growth and response to biotic/abiotic stresses. Although the AP2/ERF family has been extensively characterized in many species, very little is known about this family in ramie (Boehmeria nivea L.). In this study, 138 AP2/ERF TFs were identified from the ramie genome and were grouped into five subfamilies, including the AP2 (19), RAV (5), Soloist (1), ERF (77), and DREB (36). Unique motifs were found in the DREB/ERF subfamily members, implying significance to the AP2/ERF TF functions in these evolutionary branches. Segmental duplication events were found to play predominant roles in the BnAP2/ERF TF family expansion. Light-, stress-, and phytohormone-responsive elements were identified in the promoter region of BnAP2/ERF genes, with abscisic acid response elements (ABRE), methyl jasmonate response elements, and the dehydration response element (DRE) being dominant. The integrated transcriptome and quantitative real-time PCR (qPCR) revealed 12 key BnAP2/ERF genes positively responding to waterlogging. Five of the genes are also involved in ramet development, with two (BnERF-30 and BnERF-32) further showing multifunctional roles. The protein interaction prediction analysis further verified their crosstalk mechanism in coordinating waterlogging resistance and ramet development. Our study provides new insights into the presence of AP2/ERF TFs in ramie, and provides candidate AP2/ERF TFs for further studies on breeding varieties with coupling between water stress tolerance and high yield.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of AP2/ERF Gene Superfamily in Ramie (Boehmeria nivea L.) Revealed Their Synergistic Roles in Regulating Abiotic Stress Resistance and Ramet Development

Qiu

Zhao

Abubakar

et al. 2022

IJMS

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“…A recent study showed that ERF-type transcription factors ERF55/ERF58 play a negative role in light-regulated seed germination. Light-activated PHYA and PHYB repress the expression of ERF55/ERF58 ; furthermore, PHYA and PHYB interact with ERF55/ERF58 and inhibit their binding activity upon the downstream targets, leading to promoted seed germination [ 35 ]. These findings suggest that PHYB promotes seed germination through regulating multiple downstream light-responsive factors.…”

Section: Discussionmentioning

confidence: 99%

Histone Deacetylase HDA15 Restrains PHYB-Dependent Seed Germination via Directly Repressing GA20ox1/2 Gene Expression

Feng

Wang

et al. 2022

Cells

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Seed germination is essential for the colonization of the land plants. Light is a major environmental factor affecting seed germination, which is predominantly regulated by photoreceptor phytochrome B (PHYB). PHYB is activated by red light (designated as PHYB-on) whereas it is inactivated by far-red light (referred as PHYB-off). We previously reported that Arabidopsis histone deacetylase HDA15 interacts with phytochrome-interacting factor1 (PIF1) to repress seed germination under PHYB-off conditions. Here, we show that HDA15 plays a negative role in regulating seed germination under PHYB-on conditions. Overexpression of HDA15 in Arabidopsis restrains PHYB-dependent seed germination, while gibberellin (GA) relieves the repressive role of HDA15 under PHYB-off conditions. We further show that HDA15 directly binds to GA20ox1 and GA20ox2, two key GA biosynthesis genes and represses their expression by removal of histone H3 and H4 acetylation. Moreover, the levels of HDA15 transcript and HDA15 protein are up-regulated in the phyB mutant. Collectively, our work proposes that HDA15 acts as a negative regulator of PHYB-dependent seed germination by directly repressing GA20ox1/2 gene expression.

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“…The combined supernatant fractions were directly measured using targeted LC–MS. The LC–MS analysis was performed as described previously 55 except for using a Luna Omega Polar C18 column (3 μm; 100 Å; 150 × 0.5 mm; Phenomenex), a Luna C18(2) trap column (5 μm; 100 Å; 20 × 0.5 mm; Phenomenex) with an increased column temperature of 55 °C and a flow rate of 28 µl min −1 for the main column. The ABA content in a sample was normalized against the D6-ABA values.…”

Section: Methodsmentioning

confidence: 99%

The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence

et al. 2022

Self Cite

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Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.

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The phytochrome interacting proteins ERF55 and ERF58 repress light-induced seed germination in Arabidopsis thaliana

Cited by 30 publications

References 51 publications

Genome-Wide Analysis of AP2/ERF Gene Superfamily in Ramie (Boehmeria nivea L.) Revealed Their Synergistic Roles in Regulating Abiotic Stress Resistance and Ramet Development

Genome-Wide Analysis of AP2/ERF Gene Superfamily in Ramie (Boehmeria nivea L.) Revealed Their Synergistic Roles in Regulating Abiotic Stress Resistance and Ramet Development

Histone Deacetylase HDA15 Restrains PHYB-Dependent Seed Germination via Directly Repressing GA20ox1/2 Gene Expression

The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence

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