The Phytochrome-Interacting VASCULAR PLANT ONE–ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in <i>Arabidopsis</i>

Yasui, Yukihiko; Mukougawa, Keiko; Uemoto, Mitsuhiro; Yokofuji, Akira; Suzuri, Ryota; Nishitani, Aiko; Kohchi, Takayuki

doi:10.1105/tpc.112.101915

Cited by 87 publications

(187 citation statements)

References 96 publications

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“…17 Although the green fluorescent protein-fused VOZ2 signal was predominantly observed in the cytoplasm under normal growth conditions, nuclear-localization-signalcontaining but not nuclear-export-signal-containing VOZ2 protein can complement the late-flowering phenotype of the voz1voz2 double mutant, indicating that VOZ proteins relocate from the cytoplasm to the nucleus and regulate the flowering signaling pathways. 16 Nonetheless, no clear nuclear localization of VOZ proteins under normal growth conditions has been observed in Arabidopsis, these data indicate possible transcriptional regulatory function of VOZ proteins under particular growth conditions.…”

Section: Introductionmentioning

confidence: 77%

“…Yasui et al also reported that nuclear localization signal-containing but not nuclear export signal-containing VOZ2 protein could complement the late-flowering phenotype of the voz1voz2 double mutant, indicating that VOZ proteins function in the nucleus for regulation of flowering. 16 We previously reported that VOZ2 is degraded by the ubiquitin-proteasome system under cold-stress conditions. 17 These results suggest that VOZ proteins are transferred to the nucleus and function as transcriptional regulators when the plant cells perceive certain environmental signals (e.g., light, cold or heat), and then are rapidly subjected to the ubiquitin/proteasome-dependent degradation for inactivation.…”

Section: Discussionmentioning

confidence: 99%

“…15 Recently, VOZs were found to interact with phytochrome B and to upregulate flowering locus T (FT) and downregulate flowering locus C (FLC). 16 We previously reported that the voz1voz2 double mutant shows increased cold and drought tolerance but decreased resistance to pathogens and heat stress. 17 Although the green fluorescent protein-fused VOZ2 signal was predominantly observed in the cytoplasm under normal growth conditions, nuclear-localization-signalcontaining but not nuclear-export-signal-containing VOZ2 protein can complement the late-flowering phenotype of the voz1voz2 double mutant, indicating that VOZ proteins relocate from the cytoplasm to the nucleus and regulate the flowering signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

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Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Koguchi

Yamasaki

Hirano

et al. 2017

Plant Signaling & Behavior

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VASCULAR PLANT ONE-ZINC FINGER (VOZ)1/and VOZ2 have an ability to bind to the specific cis-element in the AVP1 promoter of Arabidopsis, which function on the PhyB-dependent flowering and possibly in various stress responses as potential transcription factors, although nuclear localization of VOZ proteins is still unclear. In this study, we found that VOZ2 is dispersed throughout the cytoplasm under normal growth conditions, whereas VOZ2 is transferred not only to the nucleus but also to the cytoplasmic foci under heat stress conditions. The VOZ2 foci predominantly co-localized with a marker of stress granules (SGs), which were cytoplasmic granular structures for mRNA storage and decay under abiotic stress conditions. We also demonstrated that GFP-VOZ2 with a nuclear localization signal was rapidly degraded via the ubiquitin/proteasome pathway under the heat stress conditions. Also, stress-related expression of DREB2A in the voz1voz2 mutant was significantly upregulated by heat stress as compared with that in the wild-type Arabidopsis. Our results suggest that VOZ2 is localized to SGs and nucleus under heat stress conditions, and functions as a transcriptional repressor of DREB2A in Arabidopsis.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Koguchi

Yamasaki

Hirano

et al. 2017

Plant Signaling & Behavior

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“…Light signals from multiple photoreceptors are integrated into the flowering pathway consisting of key factors, such as CO, FT, TWIN SISTER OF FT (TSF), SOC1, and FLOWERING LOCUS C (FLC) (8,21,22,(28)(29)(30). In the early-flowering phyB mutant, expression of FT, TSF, and SOC1 is increased (9,22).…”

Section: Significancementioning

confidence: 99%

PHYTOCHROME-DEPENDENT LATE-FLOWERING accelerates flowering through physical interactions with phytochrome B and CONSTANS

Endo

Tanigawa

Murakami

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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In flowering plants, light is one of the major environmental stimuli that determine the timing of the transition from the vegetative to reproductive phase. In Arabidopsis, phytochrome B (phyB); phyA; cryptochrome 2; and FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 are major photoreceptors that regulate flowering. Unlike phyA; cryptochrome 2; and FLAVIN-BINDING, KELCH REPEAT, F-BOX 1, phyB delays flowering mainly by destabilizing the CONSTANS (CO) protein, whose reduction leads to decreased expression of a florigen gene, FLOWERING LOCUS T. However, it remains unclear how the phyB-mediated CO destabilization is mechanistically regulated. Here, we identify a unique PHYTOCHROME-DEPENDENT LATE-FLOWERING (PHL) gene, which is mainly involved in the phyBdependent regulation of flowering. Plants with mutant phl exhibited a late-flowering phenotype, especially under long-day conditions. The late-flowering phenotype of the phl mutant was completely overridden by a phyB mutation, indicating that PHL normally accelerates flowering by countering the inhibitory effect of phyB on flowering. Accordingly, PHL physically interacted with phyB both in vitro and in vivo in a red light-dependent manner. Furthermore, in the presence of phyB under red light, PHL interacted with CO as well. Taken together, we propose that PHL regulates photoperiodic flowering by forming a phyB-PHL-CO tripartite complex.light signaling | FT

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“…Contrary to red light's role in CO protein stabilization, VASCU-LAR PLANT ONE ZINC FINGER 1 (VOZ1: At1g28520) and VOZ2 (At2g42400), two NAC (NAM, ATAF1/2 and CUC2) domain transcription factors, activate FT expression under long day conditions (Yasui et al, 2012). VOZ proteins interact with PHYB, and like PHYB, their movement between the cytoplasm and the nucleus is tightly regulated.…”

Section: Light Dependent Regulation Of Ft Transcriptionmentioning

confidence: 99%

Photoperiodic Regulation of Florigen Function inArabidopsis thaliana

Golembeski

Imaizumi

2015

The Arabidopsis Book

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One mechanism through which flowering in response to seasonal change is brought about is by sensing the fluctuation in day-length; the photoperiod. Flowering induction occurs through the production of the florigenic protein FLOWERING LOCUS T (FT) and its movement from the phloem companion cells in the leaf vasculature into the shoot apex, where meristematic reprogramming occurs. FT activation in response to photoperiod condition is accomplished largely through the activity of the transcription factor CONSTANS (CO). Regulation of CO expression and protein stability, as well as the timing of other components via the circadian clock, is a critical mechanism by which plants are able to respond to photoperiod to initiate the floral transition. Modulation of FT expression in response to external and internal stimuli via components of the flowering network is crucial to mediate a fluid flowering response to a variety of environmental parameters. In addition, the regulated movement of FT protein from the phloem to the shoot apex, and interactions that determine floral meristem cell fate, constitute novel mechanisms through which photoperiodic information is translated into flowering time.

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The Phytochrome-Interacting VASCULAR PLANT ONE–ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis

Cited by 87 publications

References 96 publications

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

PHYTOCHROME-DEPENDENT LATE-FLOWERING accelerates flowering through physical interactions with phytochrome B and CONSTANS

Photoperiodic Regulation of Florigen Function inArabidopsis thaliana

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