The plasma membrane as an adaptable fluid mosaic

Veatch, Sarah L.; Rogers, Nat; Decker, Adam; Shelby, Sarah A.

doi:10.1016/j.bbamem.2022.184114

Cited by 14 publications

(8 citation statements)

References 66 publications

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“…It is possible that this phenomenon could occur in planta as a form of functional regulation. The concept of an “adaptive membrane” that selectively modulates membrane protein function and free energy landscapes has been discussed 8 , 41 , 64 , although the lifetime of the ensuing protein regulation depends on the type of lipid-protein interaction 8 . Even so, the diversity and large quantity of lipids (along with the presence of other proteins) encompassing the membrane bilayer of a natural cell would induce diffusion and constant modification of the local lipid environment surrounding the protein as a countermeasure.…”

Section: Discussionmentioning

confidence: 99%

Functional regulation of aquaporin dynamics by lipid bilayer composition

Nguyen,

Weigle,

Shukla

2024

Nat Commun

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With the diversity of lipid-protein interactions, any observed membrane protein dynamics or functions directly depend on the lipid bilayer selection. However, the implications of lipid bilayer choice are seldom considered unless characteristic lipid-protein interactions have been previously reported. Using molecular dynamics simulation, we characterize the effects of membrane embedding on plant aquaporin SoPIP2;1, which has no reported high-affinity lipid interactions. The regulatory impacts of a realistic lipid bilayer, and nine different homogeneous bilayers, on varying SoPIP2;1 dynamics are examined. We demonstrate that SoPIP2;1’s structure, thermodynamics, kinetics, and water transport are altered as a function of each membrane construct’s ensemble properties. Notably, the realistic bilayer provides stabilization of non-functional SoPIP2;1 metastable states. Hydrophobic mismatch and lipid order parameter calculations further explain how lipid ensemble properties manipulate SoPIP2;1 behavior. Our results illustrate the importance of careful bilayer selection when studying membrane proteins. To this end, we advise cautionary measures when performing membrane protein molecular dynamics simulations.

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Section: Discussionmentioning

confidence: 99%

Functional regulation of aquaporin dynamics by lipid bilayer composition

Nguyen,

Weigle,

Shukla

2024

Nat Commun

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“…These biophysical studies on artificial cell membranes reveal a fundamental law that governs membrane organization. Based on the development of biophysical understanding, fluid mosaic models of the cell membranes have been updated [ 92 ]. The possibility of nonraft domain formation was also reported [ 93 ].…”

Section: Discussionmentioning

confidence: 99%

Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions

Shimokawa

Hamada

2023

Life

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Lateral phase separation within lipid bilayer membranes has attracted considerable attention in the fields of biophysics and cell biology. Living cells organize laterally segregated compartments, such as raft domains in an ordered phase, and regulate their dynamic structures under isothermal conditions to promote cellular functions. Model membrane systems with minimum components are powerful tools for investigating the basic phenomena of membrane phase separation. With the use of such model systems, several physicochemical characteristics of phase separation have been revealed. This review focuses on the isothermal triggering of membrane phase separation from a physical point of view. We consider the free energy of the membrane that describes lateral phase separation and explain the experimental results of model membranes to regulate domain formation under isothermal conditions. Three possible regulation factors are discussed: electrostatic interactions, chemical reactions and membrane tension. These findings may contribute to a better understanding of membrane lateral organization within living cells that function under isothermal conditions and could be useful for the development of artificial cell engineering.

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“…The initial conceptualization has evolved over the course of decades (see the 40-year ( Nicolson, 2014 ) and the more recent 50-year historical perspectives ( Bechinger, 2023 ; Nicolson and Ferreira de Mattos, 2023 )). Veatch and coworkers have also discussed the bilayer thermodynamic status as a fundamental property for tuning the composition of the membrane in response to external stimuli ( Veatch et al, 2023 ).…”

Section: Mechanisms Of Receptor Protein Modulation By Membrane Lipidsmentioning

confidence: 99%

Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids

Barrantes

2024

Front. Cell Dev. Biol.

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Membrane lipids modulate the proteins embedded in the bilayer matrix by two non-exclusive mechanisms: direct or indirect. The latter comprise those effects mediated by the physicochemical state of the membrane bilayer, whereas direct modulation entails the more specific regulatory effects transduced via recognition sites on the target membrane protein. The nicotinic acetylcholine receptor (nAChR), the paradigm member of the pentameric ligand-gated ion channel (pLGIC) superfamily of rapid neurotransmitter receptors, is modulated by both mechanisms. Reciprocally, the nAChR protein exerts influence on its surrounding interstitial lipids. Folding, conformational equilibria, ligand binding, ion permeation, topography, and diffusion of the nAChR are modulated by membrane lipids. The knowledge gained from biophysical studies of this prototypic membrane protein can be applied to other neurotransmitter receptors and most other integral membrane proteins.

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The plasma membrane as an adaptable fluid mosaic

Cited by 14 publications

References 66 publications

Functional regulation of aquaporin dynamics by lipid bilayer composition

Functional regulation of aquaporin dynamics by lipid bilayer composition

Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions

Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids

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