The plasma membrane lipid composition affects fusion between cells and model membranes

Pankov, Roumen; Markovska, Tania; Antonov, P.; Ivanova, Lidia; Momchilova, Albena

doi:10.1016/j.cbi.2006.09.010

Cited by 28 publications

(18 citation statements)

References 26 publications

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“…6 is –2χ10 -3 e /Å 2 , which corresponds to a membrane containing 10 mol% anionic lipids. This is consistent with experimentally measured plasma membrane lipid compositions [47]. For the high values of ρ in our experiments, we assume that the surface charge density of the NP, σ NP , is the same as that of the constituting lipid membrane, σ M .…”

Section: Discussionsupporting

confidence: 88%

Uptake and transfection efficiency of PEGylated cationic liposome–DNA complexes with and without RGD-tagging

et al. 2014

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Steric stabilization of cationic liposome–DNA (CL–DNA) complexes is required for in vivo applications such as gene therapy. PEGylation (PEG: poly(ethylene glycol)) of CL–DNA complexes by addition of PEG2000-lipids yields sterically stabilized nanoparticles but strongly reduces their gene delivery efficacy. PEGylation-induced weakening of the electrostatic binding of CL–DNA nanoparticles to cells (leading to reduced uptake) has been considered as a possible cause, but experimental results have been ambiguous. Using quantitative live-cell imaging in vitro, we have investigated cell attachment and uptake of PEGylated CL–DNA nanoparticles with and without a custom synthesized RGD-peptide grafted to the distal ends of PEG2000-lipids. The RGD-tagged nanoparticles exhibit strongly increased cellular attachment as well as uptake compared to nanoparticles without grafted peptide. Transfection efficiency of RGD-tagged PEGylated CL-DNA NPs increases by about an order of magnitude between NPs with low and high membrane charge density (σM; the average charge per unit area of the membrane; controlled by the molar ratio of cationic to neutral lipid), even though uptake of RGD-tagged particles is only slightly enhanced by high σM. This suggests that endosomal escape and subsequent transfection efficiency of RGD-tagged NPs is facilitated by high σM. We present a model describing the interactions between PEGylated CL–DNA nanoparticles and the anionic cell membrane which shows how the PEG grafting density and membrane charge density affect adhesion of nanoparticles to the cell surface.

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Section: Discussionsupporting

confidence: 88%

Uptake and transfection efficiency of PEGylated cationic liposome–DNA complexes with and without RGD-tagging

et al. 2014

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“…Hence, since membrane lipid composition has the potential to modulate membrane fusogenic capacity, it may also affect susceptibility to pathogens (31). …”

Section: Discussionmentioning

confidence: 99%

Chlorhexidine: beta-cyclodextrin inhibits yeast growth by extraction of ergosterol

Teixeira¹,

Araújo²,

Sinisterra³

et al. 2012

Braz. J. Microbiol.

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Chlorhexidine (Cx) augmented with beta-cyclodextrin (β-cd) inclusion compounds, termed Cx:β-cd complexes, have been developed for use as antiseptic agents. The aim of this study was to examine the interactions of Cx:β-cd complexes, prepared at different molecular ratios, with sterol and yeast membranes. The Minimal Inhibitory Concentration (MIC) against the yeast Candida albicans (C.a.) was determined for each complex; the MICs were found to range from 0.5 to 2 μg/mL. To confirm the MIC data, quantitative analysis of viable cells was performed using trypan blue staining. Mechanistic characterization of the interactions that the Cx:β-cd complexes have with the yeast membrane and assessment of membrane morphology following exposure to Cx:β-cd complexes were performed using Sterol Quantification Method analysis (SQM) and scanning electron microscopy (SEM). SQM revealed that sterol extraction increased with increasing β-cd concentrations (1.71 ×103; 1.4 ×103; 3.45 ×103, and 3.74 ×103 CFU for 1:1, 1:2, 1:3, and 1:4, respectively), likely as a consequence of membrane ergosterol solubilization. SEM images demonstrated that cell membrane damage is a visible and significant mechanism that contributes to the antimicrobial effects of Cx:β-cd complexes. Cell disorganization increased significantly as the proportion of β-cyclodextrin present in the complex increased. Morphology of cells exposed to complexes with 1:3 and 1:4 molar ratios of Cx:β-cd were observed to have large aggregates mixed with yeast remains, representing more membrane disruption than that observed in cells treated with Cx alone. In conclusion, nanoaggregates of Cx:β-cd complexes block yeast growth via ergosterol extraction, permeabilizing the membrane by creating cluster-like structures within the cell membrane, possibly due to high amounts of hydrogen bonding.

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“…Similarly, chlorhexidine induces changes in the fluidity of the yeast membrane (Teixeira et al, 2012). Antibiotic molecules must compete with phospholipids in order to interact with cholesterol and form pores (Pankov et al, 2006;Shaw et al, 2008). Potentially, variations in membrane lipid composition could be a factor that modulates membrane fusogenic capacity and affects membrane and cellular pathology (Pankov et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Antibiotic molecules must compete with phospholipids in order to interact with cholesterol and form pores (Pankov et al, 2006;Shaw et al, 2008). Potentially, variations in membrane lipid composition could be a factor that modulates membrane fusogenic capacity and affects membrane and cellular pathology (Pankov et al, 2006). Recent evidence suggests that lipid rafts can serve as docking sites for a broad range of pathogens, including viruses and bacteria (Yunomae et al, 2003;Pucadyil & Chattopadhyay, 2007;Yang et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Cyclodextrin modulates the cytotoxic effects of chlorhexidine on microrganisms and cellsin vitro

et al. 2014

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The plasma membrane lipid composition affects fusion between cells and model membranes

Cited by 28 publications

References 26 publications

Uptake and transfection efficiency of PEGylated cationic liposome–DNA complexes with and without RGD-tagging

Uptake and transfection efficiency of PEGylated cationic liposome–DNA complexes with and without RGD-tagging

Chlorhexidine: beta-cyclodextrin inhibits yeast growth by extraction of ergosterol

Cyclodextrin modulates the cytotoxic effects of chlorhexidine on microrganisms and cellsin vitro

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