The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation

Pasini, Diego; Bracken, Adrian P.; Hansen, Jacob B.; Capillo, Manuela; Helin, Kristian

doi:10.1128/mcb.01432-06

Cited by 635 publications

(706 citation statements)

References 35 publications

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“…In vitro studies of pluripotent mouse and human embryonic stem cells (ESCs) have shown that Ezh2 and other PRC2 proteins repress the transcriptions of many genes that control specific aspects of differentiation and development Lee et al, 2006;Pasini et al, 2007;Pietersen et al, 2008). Ezh2-deficient ES cells could not be viably obtained (O'Carroll et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The Expression Pattern of Polycomb Group Protein Ezh2 During Mouse Embryogenesis

Zhu

Liu

et al. 2011

The Anatomical Record

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The Polycomb group (PcG) family proteins are required for the stability of homeotic selector genes and other genes related to the regulation of mammalian development through their roles in the modulation of chromatin domains. Among them, the mammalian enhancer zeste homologue 2 (Ezh2) contributes to the transcriptional repression of these genes. Previous studies tracked the Ezh2 expression at cDNA and mRNA levels during mouse development. However, little information is known about the expression patterns of Ezh2 at the protein levels. In this study, the embryos (E6.5-E18.5) obtained through timed matings of strain Kunming mice were inserted into paraffin blocks. Tissue microarrays were constructed and followed by subsequent immunohistochemical staining. The positive cells were identified and scored based on both the percentage of stained cells and their staining intensities. Ezh2 protein expression was found throughout the embryonic tissues including the nerves, intestine epithelial, liver, pancreas, renal tubule, and lungs. Its expression level was higher at early embryonic developmental stages. However, the nerve fibers and myocardium showed weak or no immunostaining reactivities. Ezh2 protein was moderately expressed in the nuclei of renal tubule epithelial cells at E14.5. In contrast, it was weakly expressed in the fetal kidneys at E18.5 and the protein was localized in the cytoplasm of the renal tubule epithelial cells. Our data confirmed that Ezh2 protein was expressed in mouse embryos and its expression exhibited tissue specificity and dependence on the stages of embryo development. thus providing new information helpful for understanding the possible roles of Ezh2 in embryogenesis. Anat Rec, 294:1150Rec, 294: -1157Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

The Expression Pattern of Polycomb Group Protein Ezh2 During Mouse Embryogenesis

Zhu

Liu

et al. 2011

The Anatomical Record

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“…RYBP is expressed in pre-implantation mouse embryos PRC1 complexes can mediate H2AK119ub independently of H3K27me3 and PRC2 proteins. 22,40 The dynamics and localization pattern of H3K27me3 in conjunction with that of PRC2 components have been well studied in the mouse embryo. 25,27,28 Therefore, having established that H2AK119ub was present in preimplantation development, we wondered whether non-canonical PRC1 complexes contribute also to H2AK119 ubiquitylation.…”

Section: H2ak119 Monoubiquitination Is Dynamic During Preimplantationmentioning

confidence: 99%

Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis

Eid

Torres-Padilla

2016

Epigenetics

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An intense period of chromatin remodeling takes place after fertilization in mammals, which is thought necessary for epigenetic reprogramming to start a new developmental program. While much attention has been given to the role of Polycomb Repressive Complex 2 (PRC2) and to canonical PRC1 complexes during this process, little is known as to whether there is any contribution of non-canonical PRC1 in shaping the chromatin landscape after fertilization. Here, we first describe in detail the temporal dynamics and abundance of H2A ubiquitylation (H2AK119ub), a histone modification catalyzed by PRC1, during preimplantation mouse development. In addition, we have analyzed the presence of the 2 characteristic subunits of non-canonical PRC1 complexes, RYBP and its homolog YAF-2. Our results indicate that H2AK119ub is inherited from the sperm, rapidly removed from the paternal chromatin after fertilization, but detected again prior to the first mitosis, suggesting that PRC1 activity occurs as early as the zygotic stage. RYBP and YAF-2, together with the non-canonical subunit L3MBTL2, are all present during preimplantation development but show different temporal dynamics. While RYBP is absent in the zygote, it is strongly induced from the 4-cell stage onwards. YAF-2 is inherited maternally and localizes to the pericentromeric regions in the zygote, is strongly induced between the 2-and 4-cell stages but then remains weak to undetectable subsequently. All together, our data suggest that non-canonical PRC1 is active during pre-implantation development and should be regarded as an additional component during epigenetic reprogramming and in the establishment of cellular plasticity of the early embryo.

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“…Through its catalytic subunit EZH2, the PRC2 complex preferentially methylates (me) K27 of histone H3 in vitro (Cao et al 2002;Czermin et al 2002;Kuzmichev et al 2002;Müller et al 2002). However, different groups have reported that PRC2 also can methylate histone H3K9 and histone H1K26 in vitro (Czermin et al 2002;Kuzmichev et al 2002Kuzmichev et al , 2004Erhardt et al 2003;Su et al 2003;Pasini et al 2004b), but so far, genetic models only provide support for the involvement of EZH2 in catalyzing dimethylation and trimethylation of histone H3K27 in vivo (Cao and Zhang 2004;Pasini et al 2007;Puschendorf et al 2008;Riising et al 2008). The activity of the PRC2 complex is required for the association of PRC1 to target genes (Cao et al 2005;Pasini et al 2007).…”

Section: Regulation Of Stem Cell Differentiation By Histonementioning

confidence: 99%

Regulation of Stem Cell Differentiation by Histone Methyltransferases and Demethylases

Pasini

Bracken

Agger

et al. 2008

Cold Spring Harbor Symposia on Quantitative Biology

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The generation of different cell types from stem cells containing identical genetic information and their organization into tissues and organs during development is a highly complex process that requires defined transcriptional programs. Maintenance of such programs is epigenetically regulated and the factors involved in these processes are often essential for development. The activities required for cell-fate decisions are frequently deregulated in human tumors, and the elucidation of the molecular mechanisms that regulate these processes is therefore important for understanding both developmental processes and tumorigenesis.

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The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation

Cited by 635 publications

References 35 publications

The Expression Pattern of Polycomb Group Protein Ezh2 During Mouse Embryogenesis

The Expression Pattern of Polycomb Group Protein Ezh2 During Mouse Embryogenesis

Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis

Regulation of Stem Cell Differentiation by Histone Methyltransferases and Demethylases

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