THE POTENCY OF COPPER-RESISTANT BACTERIA Cupriavidus sp. IrC4 ISOLATED FROM INDUSTRIAL WASTEWATER TREATMENT PLANT IN RUNGKUT-SURABAYA AS A BIOREMEDIATION AGENT FOR HEAVY METALS

Irawati, Wahyu; Yuwono, Triwibowo; Soedarsono, Joedoro; Hartiko, Hari

doi:10.18502/kls.v2i1.179

Cited by 7 publications

(9 citation statements)

References 19 publications

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“…Meanwhile, Acinetobacter sp. IrC2 isolated from industrial sewage, Rungkut-Indonesia demonstrated multiple resistance to copper, cadmium, mercury, lead, and zinc (Irawati et al 2015).…”

Section: Isolation and Characterization Of Copper-resistant Bacteriamentioning

confidence: 99%

Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

et al. 2019

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Irawati W, Ompusunggu NP, Susilowati DN, Yuwono T. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas 20: 344-349. The use of indigenous bacteria as bioremediation agents in wastewater treatments plant is a promising solution for tackling the environmental problem. The objectives of this research were to establish the phylogenetic tree and physiological characterization of copper-resistant bacteria isolated from Cikapundung River, West Java, Indonesia. A phylogenetic tree was constructed based on 16S rDNA sequences. Physiological characterization including growth and the potency of bacterial strain for copper accumulation and copper removal was also studied. Five highly copper-resistant bacteria designated as strains CN1, CN2, CN5, CN6, and CN8 have been isolated from Cikapundung river with the MICs (Minimum Inhibitory Concentration) of 5 mM-8mM. Sequence alignment and phylogenetic analysis showed that strains CN1 and CN6 belong to Klebsiella pneumoniae with the similarity of 99%. Meanwhile, strain CN2, CN5, and CN8 belong to Acinetobacter calcoaceticus, Acinetobacter sp. and Escherichia coli with the similarity of 99%, 96%, and 95%, respectively. The highest resistant bacterial isolates were strains CN6 and CN8 with the MICs of 8 mM. The highest accumulation capability was found in strain CN1 with a total of 4.62 mg/g dry weight of cells. Meanwhile, strain CN8 demonstrated the highest copper removal with the value of 48.15%.

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“…Meanwhile, Acinetobacter sp. IrC2 isolated from industrial sewage, Rungkut-Indonesia demonstrated multiple resistance to copper, cadmium, mercury, lead, and zinc (Irawati et al 2015).…”

Section: Isolation and Characterization Of Copper-resistant Bacteriamentioning

confidence: 99%

Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

et al. 2019

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“…Appropiate concentration of heavy metal stock solution was added in autoclaved medium. LB agar was made with an addition of 2% pure agar (Irawati et al 2015).…”

Section: Growth Mediummentioning

confidence: 99%

Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia

Irawati

RIAK

SOPIAH³

et al. 2017

Biodiversitas

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Irawati W, Riak S, Sopiah N, Sulistia S. 2017. Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas 18: 1481-1486. The bacterial study is a part of human calling in preserving the earth. Many indigenous bacteria isolated from heavy metal contaminated sites had resistance to heavy metal toxicity and could be used for heavy metal removal. The aims of this study were to isolate heavy metal-tolerant indigenous bacteria from the industrial sewage of Kemisan River in Tangerang, Banten, Indonesia. The potency of bacterial isolates to remove heavy metals was also determined. The heavy-metal tolerance was determined by measuring the minimum inhibitory concentration. The potency of bacterial isolate for removing heavy metals from the medium was determined by an atomic absorption spectrophotometer. The results showed that there were eight heavy metal-resistant bacteria isolated from Kemisan River with minimum inhibitory concentration ranging from 7 mM to 11 mM. Isolate PbSI1 was the highest lead tolerant bacteria, and also tolerant to copper and zinc. The isolate was able to remove 91.25% lead, 73.38% zinc, and 98.57% copper from medium supplemented with the mixture of these heavy metals. The addition of 9 mM of lead in the medium affected the morphological appearance of isolate colonies i.e PbSI1 and PbSI3 to become darker which might occur due to the survival mechanism of bacteria by absorbing the lead inside the cells. The finding of this study indicated that isolate PbSI1 was a promising bacterium, which could be further developed for heavy metal removal.

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“…IrC1 and Acinetobacter sp. IrC2 were facilitated through the bioaccumulation of copper inside the cell up to 364.66 mg · L −1 of copper [16] It also was reported that the bacterium removed copper up to 52.98 % [24]. Previous studies showed that bacterial inoculation enhanced the ability of E. Crassipes to accumulate heavy metal.…”

Section: Copper Removalmentioning

confidence: 97%

The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

Irawati

Parhusip²,

Sopiah³

et al. 2017

KLS

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Phytoremediation is a bioremediation process using plants and microorganisms to extract, sequester, or detoxify heavy metals. Eichhornia crassipes [(Mart.) Solms] is a well-known phytoremediating plant that has the ability to remove heavy metals from water by accumulating them in their tissues. Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2 are copper resistant bacteria isolated from industrial waste in Rungkut, Surabaya. The aim of this research was to study the effect of Acinetobacter sp. IrC1 and Acinetobacter sp. IrC2 inoculation in copper phytoremediation process using Eichhornia crassipes. Bacterial isolate with colony form unit of 10 8 was inoculated into the rhizosphere of Eichhornia crassipes in water containing 10 mL · L −1 and 20 mL · L

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THE POTENCY OF COPPER-RESISTANT BACTERIA Cupriavidus sp. IrC4 ISOLATED FROM INDUSTRIAL WASTEWATER TREATMENT PLANT IN RUNGKUT-SURABAYA AS A BIOREMEDIATION AGENT FOR HEAVY METALS

Cited by 7 publications

References 19 publications

Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia

The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp. in Copper Phytoremediation using Eichhornia crasippes [(Mart.) Solms]

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