The potential mechanism for Hydroxysafflor yellow A attenuating blood-brain barrier dysfunction via tight junction signaling pathways excavated by an integrated serial affinity chromatography and shotgun proteomics analysis approach

Lv, Yanni; Fu, Longsheng

doi:10.1016/j.neuint.2017.10.012

Cited by 22 publications

(17 citation statements)

References 41 publications

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“…When damage occurs, SAA can reach the brain through the BBB and induce therapeutic effects in cerebral I/R model rats. In addition, studies have shown that HSYA can cross the BBB, resulting in downregulation of 12/15-lipoxygenase (12/15-LOX) and its metabolic products (Sun et al, 2012), and attenuation of occludin, claudin-5, and ZO-1 expressions, resulting in BBB permeability and improvement of tight junctions in MCAO mice (Lv and Fu, 2018). Meanwhile, DSS can readily permeate the BBB when normal rats were orally administered of Danshen extract .…”

Section: Discussionmentioning

confidence: 99%

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Zhao

Chen

et al. 2020

Front. Pharmacol.

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Background: In China, the combination of herb Salvia miltiorrhiza Bge. (Danshen) and Carthamus tinctorius L. (Honghua) is an effective treatment for stroke. A previous study showed that the combination of four herbal components: danshensu (DSS), hydroxysafflor yellow A (HSYA), salvianolic acid A (SAA), and salvianolic acid B (SAB) was effective for treatment of cerebral ischemia-reperfusion (I/R) injury in rats. However, the pharmacokinetic characteristics of this formula require further investigation. The present study investigated the pharmacokinetic differences between each component of in two formulas in cerebral I/R injury rats. The influencing factors may affect the compatibility of components were analyzed.Methods: Focal cerebral I/R was induced by middle cerebral artery occlusion (MCAO). Rats that underwent MCAO were randomly divided into two groups and administered treatments through the tail vein. Blood samples were collected at predetermined time points following administration. The concentrations of DSS, HSYA, SAB, and SAA in rat plasma were determined using HPLC-DAD, and the main pharmacokinetic parameters were calculated. Pharmacokinetic parameters were calculated using DAS 3.2.6 software and SPSS 23.0 statistical analysis software.Results: Our results showed that DSS, HSYA, SAB, and SAA in MCAO model rats had statistically significant differences in two formulas. For DSS and SAA, pharmacokinetic parameters with statistically significant differences including AUC (0−t) , AUMC (0−t) , MRT (0−t) , VRT (0−t) , t 1/2z , V z , CL z , and C max (P < 0.01). For HSYA, significant differences in the parameters including AUC (0−t) , AUMC (0−t) , MRT (0−t) , VRT (0−t) (P < 0.01), CL z and C max (P < 0.05). Conclusion:The difference in pharmacokinetic parameters in response to each component may have been due to differences in the dosages of the components (HSYA, SAA, SAB) and the compatibility of components. Meanwhile, there were many Frontiers in Pharmacology | www.frontiersin.org January 2020 | Volume 10 | Article 1598 1

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Section: Discussionmentioning

confidence: 99%

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Zhao

Chen

et al. 2020

Front. Pharmacol.

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“…1,25‐Dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ), the physiologically active form of vitamin D, has been found to enhance the clearance of Aβ across BBB via regulating the expression of LRP1 and RAGE (Guo et al, ). Hydroxysafflor yellow A can attenuate BBB dysfunction via TJ signaling pathways (Lv & Fu, ). So in this study, we investigated the effect of catalpol on fibrillar Aβ 1–42 ‐induced changes in an in vitro BBB model by analyzing cell toxicity, BBB permeability, TJ scaffold proteins, degrading proteins, Aβ transport protein expression, and apoptotic signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Catalpol provides a protective effect on fibrillary Aβ_1–42‐induced barrier disruption in an in vitro model of the blood–brain barrier

Liu

Kang

et al. 2018

Phytotherapy Research

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Excessive amyloid beta (Aβ) deposition in brain is mainly responsible for cell damage and blood-brain barrier (BBB) disruption in Alzheimer's disease (AD). Catalpol, an iridoid glucoside extracted from the root of Rehmannia glutinosa Libosch, has neuroprotective effect against AD. It is unclear whether catalpol has a protective effect on Aβ-induced BBB leakage. We employed an immortalized endothelial cell line (bEnd.3) and astrocytes co-culture to mimic a BBB model in vitro and investigated the effect of catalpol on BBB. We found that treatment with catalpol decreased BBB hyperpermeability induced by fibrillar Aβ . Data from western blotting showed that catalpol prevented fibrillar Aβ -induced bEnd.3 cell apoptosis through mitochondria-dependent and death receptor pathways; decreased the levels of matrix metalloproteinases (MMPs), MMP-2, MMP-9, and the receptor for advanced glycation end products; and increased the levels of tight junction proteins (ZO-1, occludin, and claudin-5), low-density lipoprotein receptor-related protein 1, and P-glycoprotein in fibrillar Aβ -treated bEnd.3 cells. Moreover, catalpol also enhanced soluble Aβ efflux across the fibrillar Aβ -treated bEnd.3 cells BBB monolayer model. Altogether, our results suggest that catalpol alleviate fibrillar Aβ -induced BBB disruption, enhance soluble Aβ clearance, and offer a feasible therapeutic application in AD treatment.

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“…The blood-brain barrier (BBB) essentially maintains a stable cerebral homeostasis, while the destruction or increased permeability of BBB are common pathological processes during many serious cerebrovascular diseases . A study by Lv et al revealed that HSYA significantly attenuated BBB dysfunction in anti-inflammatory patterns in ischemia stroke via the tight junction pathway, especially the NMMHC IIA, TLR4/ PI3K/Akt/Jun N-terminal kinase 1/2/14-3-3ϵ pathway while inhibiting the expressions of occludin, claudin-5, and zonula occludens-1 (Lv and Fu, 2018). Since MMPs are the main endoproteinases involved in BBB destruction (Romanic et al, 1998), the prominent inhibitory effects of HSYA on MMP-2 and MMP-9 mentioned in cardiovascular diseases may also contribute to BBB improvement.…”

Section: Effects On Cerebral Ischemia (Ci)mentioning

confidence: 99%

Therapeutic Potential of Hydroxysafflor Yellow A on Cardio-Cerebrovascular Diseases

Bai

Wang

et al. 2020

Front. Pharmacol.

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The incidence rate of cardio-cerebrovascular diseases (CCVDs) is increasing worldwide, causing an increasingly serious public health burden. The pursuit of new promising treatment options is thus becoming a pressing issue. Hydroxysafflor yellow A (HSYA) is one of the main active quinochalcone C-glycosides in the florets of Carthamus tinctorius L., a medical and edible dual-purpose plant. HSYA has attracted much interest for its pharmacological actions in treating and/or managing CCVDs, such as myocardial and cerebral ischemia, hypertension, atherosclerosis, vascular dementia, and traumatic brain injury, in massive preclinical studies. In this review, we briefly summarized the mode and mechanism of action of HSYA on CCVDs based on these preclinical studies. The therapeutic effects of HSYA against CCVDs were presumed to reside mostly in its antioxidant, anti-inflammatory, and neuroprotective roles by acting on complex signaling pathways.

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The potential mechanism for Hydroxysafflor yellow A attenuating blood-brain barrier dysfunction via tight junction signaling pathways excavated by an integrated serial affinity chromatography and shotgun proteomics analysis approach

Cited by 22 publications

References 41 publications

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Catalpol provides a protective effect on fibrillary Aβ_1–42‐induced barrier disruption in an in vitro model of the blood–brain barrier

Therapeutic Potential of Hydroxysafflor Yellow A on Cardio-Cerebrovascular Diseases

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The potential mechanism for Hydroxysafflor yellow A attenuating blood-brain barrier dysfunction via tight junction signaling pathways excavated by an integrated serial affinity chromatography and shotgun proteomics analysis approach

Cited by 22 publications

References 41 publications

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Catalpol provides a protective effect on fibrillary Aβ1–42‐induced barrier disruption in an in vitro model of the blood–brain barrier

Therapeutic Potential of Hydroxysafflor Yellow A on Cardio-Cerebrovascular Diseases

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Catalpol provides a protective effect on fibrillary Aβ_1–42‐induced barrier disruption in an in vitro model of the blood–brain barrier