The potential of zein nanoparticles to protect entrapped β-carotene in the presence of milk under simulated gastrointestinal (GI) conditions

Abstract

a b s t r a c tThe addition of nanoentrapped b-carotene in milk is proposed as a valid method to improve stability and enhance antioxidant activity of b-carotene during GI transit. This study focused on two types of delivery systems, nanoparticles and nanoemulsions, and compared their ability to enhance the physicochemical stability and antioxidant activity of b-carotene in the presence of milk under simulated GI conditions. bCarotene-loaded zein nanoparticles and nanoemulsion, stabilized by pluronic F127 and lecithin, were synthesized and added to liquid milk. Degradation of b-carotene was spectrophotometrically analyzed, and antioxidant activity was measured using ABTS and TBARS assays in simulated gastric fluid (SGF) with pepsin for 2 h and in simulated intestinal fluid (SIF) with pancreatin for 24 h. The initial amount of bcarotene remained at 18.30 ± 1.29% and 4.41 ± 2.51% after a 26 h total time of GI exposure in nanoentrapped and emulsified forms, respectively. In both systems, nanoentrapment proved to be beneficial to beta-carotene stability, but this benefit was decreased in the presence of milk. The improvement in antioxidant activity of nanodelivered b-carotene was confirmed by ABTS and TBARS assays. In conclusion, zein nanoparticles improved chemical stability and antioxidant activity of entrapped beta-carotene versus emulsions in the presence of milk under simulated gastrointestinal environments.

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

“…Recently, zein nanoparticles have been loaded with different compounds such as resveratrol (Liang et al, 2018), curcumin (Zou et al, 2016), epigallocatechin gallate (Donsì, Voudouris, Veen, & Velikov, 2017), rutin (Zhang & Zhao, 2017), and β-carotene (Chuacharoen & Sabliov, 2016); these studies showed that this protein matrix possesses a high capability to encapsulate these nutraceutical compounds. Furthermore, antioxidant activity and bioaccessibility increase and chemical degradation decreases in the gastrointestinal tract; therefore, its high bioactivity may be compared with unencapsulated compounds.…”

Resveratrol encapsulation in core-shell biopolymer nanoparticles: Impact on antioxidant and anticancer activities

“…Zein nanoparticle encapsulation protected resveratrol from isomerization by UV light (Davidov-Pardo, Joye, Espinal-Ruiz, & McClements, 2015), and increased its oral bioavailability (Penalva, Esparza, Larraneta, Gonzalez-Navarro, Gamazo, & Irache, 2015). Zein nanoparticles have also been shown to be useful for encapsulating other types of hydrophobic nutraceuticals, including curcumin (Patel, Hu, Tiwari, & Velikov, 2010;Zou, Zheng, Zhang, Zhang, Liu, Liu, et al, 2016), retinol (Park, Park, & Kim, 2015), peppermint oil (Chen & Zhong, 2015), quercetin (Patel, Heussen, Hazekamp, Drost, Velikov, 2012) and carotenoids (Chuacharoen & Sabliov, 2016a, 2016b.…”

Functional and Engineered Colloids from Edible Materials for Emerging Applications in Designing the Food of the Future

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