The PR‐10 Protein Pru p 1 is an Endonuclease that Preferentially Cleaves Single–Stranded RNA
Manuel Röck,
Sarah Viola Heel,
Fabian Sebastian Juen
et al.
Abstract:Pathogenesis‐related class 10 (PR‐10) proteins play a crucial role in plant defense by acting as ribonucleases. The specific mechanism of action and substrate specificity of these proteins have remained largely unexplored so far. In this study, we elucidate the enzymatic activity of Pru p 1, a PR‐10 protein from peach. We demonstrate that this protein catalyzes the endonucleolytic backbone cleavage of RNA substrates into short oligonucleotides. Initial cleavage products, identified through kinetic analysis, ca… Show more
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