The precipitation of polyanions by long-chain aliphatic ammonium compounds IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support

Antonopoulos, C.A.; Borelius, Elisabeth; Gardell, S.; Hamnström, B.; Scott, J. E.

doi:10.1016/0006-3002(61)90360-2

Cited by 155 publications

(33 citation statements)

References 15 publications

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“…Cartilage was digested with papain and the hyaluronic acid was purified by precipitation with cetylpyridinium chloride and chromatography on a cetylpyridinium chloride-cellulose column (Antonopoulos et al, 1961). The purified sample contained equimolar amounts of hexosamine and uronic acid, with 80% of the hexosamines being glucosamine.…”

Section: Resultsmentioning

confidence: 99%

“…for 30min and the pellet was freeze-dried. The sample was redissolved in 0.02M-Na2SO4 and applied to a cetylpyridinium chloride-cellulose column (2cm x 30cm) equilibrated with 1 % cetylpyridinium chloride as described by Antonopoulos et al (1961). The column was eluted with 4 bed volumes of 1 % cetylpyridinium chloride, and the effluent was discarded.…”

Section: Chemical Methodsmentioning

confidence: 99%

“…The pellet was washed with ethanol and freeze-dried. Since hyaluronic acid is eluted from a cetylpyridinium chloride-cellulose column with 0.3 MNaCl (Antonopoulos et al, 1961) this material will be referred to as the hyaluronic acid fraction. Its weight was 9.8 mg. Hexosamines were separated on an automatic amino acid analyser after hydrolysis of the samples in 4M-HCl under N2 for lOh at 100°C.…”

Section: Chemical Methodsmentioning

confidence: 99%

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Cartilage proteoglycan aggregates. Electronmicroscopic studies of native and fragmented molecules

Heinegård

Lohmander

Thyberg

1978

Biochemical Journal

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1. Proteoglycan aggregates from bovine nasal cartilage were studied by using electron microscopy of proteoglycan/cytochrome c monolayers. 2. The aggregates contained a variably long central filament of hyaluronic acid with an average length of 1037nm. The proteoglycan monomers attached to the hyaluronic acid appeared as side chain filaments varying in length (averaging 249nm). They were distributed along the central filament at an average distance of about 36nm. 3. Chondroitin sulphate side chains were removed from the proteoglycan monomers of the aggregates by partial chondroitinase digestion. The molecules obtained had the same general appearance as intact aggregates. 4. Proteoglycan aggregates were treated with trypsin and the largest fragment, which contains the hyaluronic acid, link protein and hyaluronic acid-binding region, was recovered and studied with electron microscopy. Filaments that lacked the side chain extensions and had the same length as the central filament in the intact aggregate were observed. 5. Hyaluronic acid isolated after papain digestion of cartilage extracts gave filaments with similar length and size distribution as observed for the central filament both in the intact aggregate and in the trypsin digests. 6. Umbilical-cord hyaluronic acid was also studied and gave electron micrographs similar to those described for hyaluronic acid from cartilage. However, the length of the filament was somewhat shorter. 7. The electron micrographs of both intact and selectively degraded proteoglycans corroborate the current model of cartilage proteoglycan structure.

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Section: Resultsmentioning

confidence: 99%

Section: Chemical Methodsmentioning

confidence: 99%

Section: Chemical Methodsmentioning

confidence: 99%

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Cartilage proteoglycan aggregates. Electronmicroscopic studies of native and fragmented molecules

Heinegård

Lohmander

Thyberg

1978

Biochemical Journal

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“…However, these works with acid mucopolysaccharides have been done using quaternary ammonium salts, such as cetyltrimethyl ammonium bromide or cetylpyridinium chloride (CPC) and dialytic procedure. High solubility mainly of keratosulfate in the presence of excess quaternary ammonium salt and loss of urinary acid mucopolysaccharides during dialysis are indicated by several workers (Antonopoulos et al 1961, DI Ferrante 1967, Muir et al 1963, Thompson andCastor 1966, .Constantopoulos 1968). For these reasons, pattern of urinary acid mucopolysaccharide excretion in normal male children has been also quantitatively studied using ECTEOLA-cellulose and Sephadex G-25 columns instead of quaternary ammonium salt and dialytic procedure.…”

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confidence: 95%

Pattern of Urinary Acid Mucopolysaccharide Excretion in Normal Male Children

Orii

Minami

Nakao

1972

Tohoku J. Exp. Med.

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“…The 0-25°/0 ethanol precipitate (670 mg) was subsequently fractionated on a column (4 x 50 cm) of cellulose equilibrated with lo/o aqueous cetylpyridinium chloride-0.005 M NaSO, [5]. The column was eluted stepwise with 1 liter each of the above solution, followed by 0.9 M MgC1,-0.1 M acetic acid (A), 1.0 M MgCh-0.1 M acetic acid (B), and 2.0M NaCl (C); all the salt solutions also contained 0.05°/0 cetylpyridinium chloride.…”

Section: Preparation and Practionation Of Dermatan Sulfatementioning

confidence: 99%

Structure of Pig Skin Dermatan Suflate

Fransson

Malmström

1971

European Journal of Biochemistry

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Thee copolymeric structure of pig skin dermatan sulfate was further studied with particular referene to residual glucuronic acid moieties present in the molecule after sequential treatment with testicular hyaluronidase and β‐glucuronidase. Subsequent treatment with chondroitinase‐AC lead to a further release of glucuronic acid in the form of disaccharides, tetrasaccharidies and higher oligosaccharides/glycopeptides. Since these products contained nonreducing teminal, unsaturated uronic acid residues, it was concluded that some of the residual glucuronic acid moieties were located in ultimate or penultimate reducing terminal chondroitin 4‐sulfate repeating periods of fragments obtained after cleavage with testicular hyalyronidase. Additional, single chondroitin 4‐sulfate repeating periods were present in the vicinity of the carbohydrate‐protein linkage region or elsewhere in the chain. The former periods were regularly obseved 2‐3 units apart from the linkage region whereas the latter periods occurred preferentially in sequences like…BBBBABABAAAA…(A and B are chondroitin 4‐sulfate and dermatan sulfate repeating periods, respectively).

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The precipitation of polyanions by long-chain aliphatic ammonium compounds IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support

Cited by 155 publications

References 15 publications

Cartilage proteoglycan aggregates. Electronmicroscopic studies of native and fragmented molecules

Cartilage proteoglycan aggregates. Electronmicroscopic studies of native and fragmented molecules

Pattern of Urinary Acid Mucopolysaccharide Excretion in Normal Male Children

Structure of Pig Skin Dermatan Suflate

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