The predicted DBL oncogene product defines a distinct class of transforming proteins.

Eva, Alessandra; Vecchio, Giancarlo; Rao, C D; Tronick, Steven R.; Aaronson, Stuart A.

doi:10.1073/pnas.85.7.2061

Cited by 53 publications

(36 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate the specificity of Sos for Ras activation in T cells, we compared the activity of Sos to that of the Dbl oncoprotein, a GEF specific for members of the Rho/Rac subfamily of small GTP-binding proteins (22)(23)(24). In addition, we investigated the activity of the product of the Vav protooncogene (25), a hematopoietic-specific protein implicated in a variety of signal transduction pathways (reviewed in ref.…”

Section: Resultsmentioning

confidence: 99%

Signal transduction in T lymphocytes using a conditional allele of Sos.

Holsinger

Spencer²,

Austin³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

120

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Signal transduction in T lymphocytes using a conditional allele of Sos.

Holsinger

Spencer²,

Austin³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

120

View full text Add to dashboard Cite

show abstract

“…Expression plasmids containing these GEF Rho have been described: FGD1 (20), Dbl and pDbl (proto-Dbl) (23,28), Tiam1 and dN-Tiam1, aa 386 -1591 (16). GFKG1 was subcloned (nucleotides 132-2384 of the original cDNA) into pCMV6M (19).…”

Section: Methodsmentioning

confidence: 99%

Guanine Nucleotide Exchange Factors Regulate Specificity of Downstream Signaling from Rac and Cdc42

Zhou¹,

Wang²,

Gorski³

et al. 1998

Journal of Biological Chemistry

View full text Add to dashboard Cite

The Rac and Cdc42 GTPases regulate diverse cellular behaviors involving the actin cytoskeleton, gene transcription, and the activity of multiple protein and lipid kinases. All of these pathways can potentially become activated when GTP-Rac or GTP-Cdc42 is formed in response to external cell signals, yet it is evident that each activity must also be able to be controlled individually. The mechanisms by which such specificity of GTPase signaling in response to upstream stimuli is achieved remains unclear. We investigated the action of several well characterized guanine nucleotide exchange factors (GEF Rho ) to activate Rac-and/or Cdc42-dependent kinase pathways. Coexpression studies in COS-7 cells revealed that the ability of individual guanine nucleotide exchange factors (GEFs) to activate the p21-activated kinase PAK1 could be dissociated from activation of c-Jun amino-terminal kinase, even though activation of both pathways requires the action of the GEFs on Rac and/or Cdc42. In contrast, expression of constitutively active forms of Rac or Cdc42 effectively stimulated both downstream kinases. We conclude that GEFs can be important determinants of downstream signaling specificity for members of the Rho GTPase family.

show abstract

“…The first mammalian Rho GEF, Dbl, was identified as a transforming gene in an NIH3T3 focus formation assay using DNA from a cell line of human diffuse B-cell lymphoma. Since then, over 70 distinct members of this Dbl family have been identified in humans (115). GEFs for the large number of different Ras superfamily proteins vary drastically in structure, but there are significant similarities.…”

Section: B the Gdp-gtp Cycle And Regulatory Proteinsmentioning

confidence: 99%

Small G Proteins in the Cardiovascular System: Physiological and Pathological Aspects

Loirand

Sauzeau

Pacaud

2013

Physiological Reviews

View full text Add to dashboard Cite

Small G proteins exist in eukaryotes from yeast to human and constitute the Ras superfamily comprising more than 100 members. This superfamily is structurally classified into five families: the Ras, Rho, Rab, Arf, and Ran families that control a wide variety of cell and biological functions through highly coordinated regulation processes. Increasing evidence has accumulated to identify small G proteins and their regulators as key players of the cardiovascular physiology that control a large panel of cardiac (heart rhythm, contraction, hypertrophy) and vascular functions (angiogenesis, vascular permeability, vasoconstriction). Indeed, basal Ras protein activity is required for homeostatic functions in physiological conditions, but sustained overactivation of Ras proteins or spatiotemporal dysregulation of Ras signaling pathways has pathological consequences in the cardiovascular system. The primary object of this review is to provide a comprehensive overview of the current progress in our understanding of the role of small G proteins and their regulators in cardiovascular physiology and pathologies.

show abstract

The predicted DBL oncogene product defines a distinct class of transforming proteins.

Abstract: The DBL

Cited by 53 publications

References 48 publications

Signal transduction in T lymphocytes using a conditional allele of Sos.

Signal transduction in T lymphocytes using a conditional allele of Sos.

Guanine Nucleotide Exchange Factors Regulate Specificity of Downstream Signaling from Rac and Cdc42

Small G Proteins in the Cardiovascular System: Physiological and Pathological Aspects

Contact Info

Product

Resources

About