The Predictive Value of Circulating Tumor Cells in Ovarian Cancer: A Meta Analysis

Zeng, Liqiong; Liang, Xiaolong; Q, Liu; Yang, Zhu

doi:10.1097/igc.0000000000000459

Cited by 18 publications

(17 citation statements)

References 48 publications

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“…In the PB group, it showed that patients with positive CTCs had significantly shorter overall survival (OS) and disease-free survival (DFS) than patients with negative CTCs (HR, 2.09; CI, 1.13-3.88 and HR, 1.72; CI, 1.32-2.25, respectively). The same result was shown with DTCs in the BM group (HR, 1.61; CI, 1.27-2.04 and HR, 1.44; CI, 1.15-1.80, respectively) [305].…”

Section: Other Cancerssupporting

confidence: 79%

The Selection Strategy for Circulating Tumor Cells (CTCs) Isolation and Enumeration: Technical Features, Methods, and Clinical Applications

Hsieh¹,

Wu²

2016

Tumor Metastasis

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The key aim of the proposed chapter is to provide readers a brief description for the most important parts of the field of circulating tumor cells (CTCs): the core techniques, including negative and positive selection-based CTC isolation, and the differences between them. Most importantly, we will also review the clinical applications and important findings in clinical trials. The evidence-based review will not only help clinicians use CTCs to predict recurrence and foresee the disease-related outcomes but also to inspire the researchers in this field to conduct further investigations.

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Section: Other Cancerssupporting

confidence: 79%

The Selection Strategy for Circulating Tumor Cells (CTCs) Isolation and Enumeration: Technical Features, Methods, and Clinical Applications

Hsieh¹,

Wu²

2016

Tumor Metastasis

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“…8,9 Three aspects of selection, comparability, and exposure (9 scores in total) were carefully judged. Studies of moderate or high quality were included (score of 5 or higher).…”

Section: Data Extraction and Quality Assessmentmentioning

confidence: 99%

Gene Polymorphisms of Toll-Like Receptor 9 −1486T/C and 2848G/A in Cervical Cancer Risk

Zhao

Yuan

et al. 2015

Int J Gynecol Cancer

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“… 5 Although some smaller studies found no correlation of CTCs with disease progression, four recent meta-analyses showed that CTC positivity in ovarian cancer patients was significantly associated with shorter overall, disease-free, and progression-free survival as well as advanced stage in ovarian cancer. 6 – 9 …”

Section: Introductionmentioning

confidence: 99%

Improved ovarian cancer EMT-CTC isolation by immunomagnetic targeting of epithelial EpCAM and mesenchymal N-cadherin

Roohullah

Lynch

et al. 2018

Journal of Circulating Biomarkers

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Epithelial cell adhesion molecule (EpCAM)-targeted capture remains the most common isolation strategy for circulating tumor cells (CTCs). However, epithelial-to-mesenchymal transition (EMT) leads to decreased epithelial EpCAM expression affecting the optimal CTC capture. In this study, we tested a cohort of ovarian cancer cell lines using flow cytometry to identify N-cadherin as the additional immunomagnetic cell surface target for ovarian cancer cell isolation. Combined immunomagnetic targeting of mesenchymal N-cadherin and epithelial EpCAM enriched CTCs from advanced ovarian cancer patient blood approximately three times more efficiently than targeting of EpCAM alone. We also show that more EMT-phenotype CTCs are captured by including N-cadherin targeting into CTC isolation protocols. However, after N-cadherin-based CTC isolation, in some blood samples of healthy individuals, we also observed the presence of cells expressing markers common to CTCs. Our data show that these “false positives” can be largely distinguished from CTCs as circulating endothelial cells (CECs) by vascular endothelial–cadherin co-staining. CEC counts are highly variable in patients and healthy controls. Our data demonstrate that a combination of EpCAM with N-cadherin-targeted isolation can improve CTC detection and widen the EMT-phenotype spectrum of captured CTCs.

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The Predictive Value of Circulating Tumor Cells in Ovarian Cancer: A Meta Analysis

Cited by 18 publications

References 48 publications

The Selection Strategy for Circulating Tumor Cells (CTCs) Isolation and Enumeration: Technical Features, Methods, and Clinical Applications

The Selection Strategy for Circulating Tumor Cells (CTCs) Isolation and Enumeration: Technical Features, Methods, and Clinical Applications

Gene Polymorphisms of Toll-Like Receptor 9 −1486T/C and 2848G/A in Cervical Cancer Risk

Improved ovarian cancer EMT-CTC isolation by immunomagnetic targeting of epithelial EpCAM and mesenchymal N-cadherin

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