The preparation of the ordered pores colloidal crystal scaffold and its role in promoting growth of lung cells

Ban, Qing; Chen, Wuya; Du, Shiwei; Wang, Huimin; Li, Jian; You, Rong; Jin, Ying; Xue, Yongyong; Guan, Yan‐Qing

doi:10.1016/j.colsurfb.2018.10.055

Cited by 4 publications

(3 citation statements)

References 50 publications

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“…Highly ordered porous polymer structures have great potential in biomedical application. In TE, the pore architectures of the porous film affected the extent of cellar behaviors, including adhesion, proliferation, migration, differentiation [ 25 , 100 , 101 ]. For example, the morphology and functionality of cardiac myocytes, neural progenitor cells, and endothelial cells were manipulated by altering the size and shape of the micropores of the honeycomb films [ 102 ].…”

Section: Microfluidic Scaffolds and Biomedical Applicationsmentioning

confidence: 99%

Microfluidic Technology for the Production of Well-Ordered Porous Polymer Scaffolds

Zhao

Wang

et al. 2020

Polymers

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Advances in tissue engineering (TE) have revealed that porosity architectures, such as pore shape, pore size and pore interconnectivity are the key morphological properties of scaffolds. Well-ordered porous polymer scaffolds, which have uniform pore size, regular geometric shape, high porosity and good pore interconnectivity, facilitate the loading and distribution of active biomolecules, as well as cell adhesion, proliferation and migration. However, these are difficult to prepare by traditional methods and the existing well-ordered porous scaffold preparation methods require expensive experimental equipment or cumbersome preparation steps. Generally, droplet-based microfluidics, which generates and manipulates discrete droplets through immiscible multiphase flows inside microchannels, has emerged as a versatile tool for generation of well-ordered porous materials. This short review details this novel method and the latest developments in well-ordered porous scaffold preparation via microfluidic technology. The pore structure and properties of microfluidic scaffolds are discussed in depth, laying the foundation for further research and application in TE. Furthermore, we outline the bottlenecks and future developments in this particular field, and a brief outlook on the future development of microfluidic technique for scaffold fabrication is presented.

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Section: Microfluidic Scaffolds and Biomedical Applicationsmentioning

confidence: 99%

Microfluidic Technology for the Production of Well-Ordered Porous Polymer Scaffolds

Zhao

Wang

et al. 2020

Polymers

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“…[ 20,21 ] Even so, many studies with BEAS‐2B cells and nanotoxicity utilize over 2% serum in the cell culture medium, potentially causing conflicting results ( Figure 1 ). [ 22–44 ]…”

Section: Introductionmentioning

confidence: 99%

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Morris

Givens

Silva

et al. 2021

Advanced NanoBiomed Research

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Copper oxide (CuO) nanoparticles (NPs) are abundant in manufacturing processes, but they are an airway irritant. In vitro pulmonary toxicity of CuO NPs has been modeled using cell lines such as human bronchial epithelial cell line BEAS‐2B. In 2D in vitro culture, BEAS‐2B undergoes squamous differentiation due to the presence of serum. Differentiation is part of the repair process of lung cells in vivo that helps to preserve the epithelial lining of the respiratory tract. Herein, the effects of serum on the hydrodynamic diameter, cellular viability, cellular differentiation, and cellular uptake of 5 and 35 nm CuO NPs are investigated, and the mean cell area is used as the differentiation marker for BEAS‐2B cells. The results demonstrate that the hydrodynamic diameter decreases with the addition of serum to the culture medium. Serum also increases the mean cell area, and only affects dose‐dependent cytotoxicity of 35 nm CuO NPs, while simultaneously having no effect on intracellular Cu2+. This study presents evidence that both NP size and the presence of serum in culture media influence the relative viability of BEAS‐2B cells following CuO NP exposure and highlights a critical need for carefully designed experiments and accurately reported conditions.

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“…Chitosan [125] Bone TE PCL 148 1% w/v Physical Chitosan [169] TE PLCA NA 2% w/v Physical Chitosan [170] Wound healing SiO2 NA 4 % w/v Glutaraldehyde Chitin/ chitosan [171,172] Cartilage TE PS 160 3% w/v Genipin Chitosan/ gelatin [173] Lung TE Fe3O4 MNPs NA Chitosan: gelatin 1:2 Genipin Chitosan/ gelatin [174] Spinal cord injury PS 160…”

Section: Genipinmentioning

confidence: 99%

Three-dimensional artificial liver platforms for drug screening and tissue engineering applications

Ferracci¹

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also from NCCS, for her precious help, thorough comments, smart suggestions and warm encouragement.A special thanks goes to Dr. Bae-Hoon Lee, from Wenzhou Institute of Biomaterials and Engineering, for his knowledgeable and insightful advice and comments, his constant guidance and his encouragement to always look for new research areas to explore. I'm extremely grateful I had the opportunity to work with him.A particular thanks also to all my colleagues from the ETS group, for their help, advice and encouragement, as well as for all the laugh and cheerful moments; it has been a pleasure to be part of such an international, multidisciplinary and inspiring group of people.Last, but not least, I'd like to thank my family, who believed in me and supported me to push my boundaries, and my friends in Singapore, for their mental support and for making this PhD journey an amazing experience.

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The preparation of the ordered pores colloidal crystal scaffold and its role in promoting growth of lung cells

Cited by 4 publications

References 50 publications

Microfluidic Technology for the Production of Well-Ordered Porous Polymer Scaffolds

Microfluidic Technology for the Production of Well-Ordered Porous Polymer Scaffolds

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Three-dimensional artificial liver platforms for drug screening and tissue engineering applications

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