The presence of Prevotella melaninogenica within tissue and preliminary study on its role in the pathogenesis of oral lichen planus

Abstract

Objective Oral lichen planus (OLP) is a chronic inflammatory disease that occurs in the oral mucosa with characteristic white striations lesions, recurrent erosions, and pains. The etiology and pathogenesis of OLP are still unclear. Materials and Methods We analyzed the bacterial community structure of buccal mucosa in patients with OLP and normal controls by high‐throughput sequencing. Fluorescence in situ hybridization (FISH) was used to detect Prevotella melaninogenica (P. melaninogenica) in 13 OLP samples and 10 controls. The amounts of P. melaninogenica in OLP buccal mucosa and the expression of inflammatory cytokines in co‐culture of mouse‐derived macrophages with P. melaninogenica were detected by RT‐qPCR. Results The P. melaninogenica was more abundant in OLP than in healthy controls, and the differences were significant at the level of the phylum, family, genus, and species (p < .05). FISH showed that P. melaninogenica can invade the epithelium and even the lamina propria of OLP, while no invasion was found in the normal mucosa. Prevotella melaninogenica can adhere to and invade macrophages and then activate the transcription of IL‐1β, IL‐6, and TNF‐α in NF‐κB signaling pathway. Conclusion Prevotella melaninogenica may be involved in the pathogenic process of OLP, and its specific mechanism deserves further study.

Streptococcus mitis and Prevotella melaninogenica Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients

“…Contrary to our findings, Robayo and coworkers did not detect P. melaninogenica in oropharyngeal carcinoma tissue samples [39], but de Martin et al confirmed its presence in the tonsillar OSCC [40]. A recent study by Zheng et al using in situ hybridization established the presence of P. melaninogenica in oral lichen planus [41].…”

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

“…Oral lichen planus (OLP) is characterized as a chronic inflammatory condition affecting the oral mucosa with white striated, non-erosive or erosive lesions. In 13 OLP cases, the abundance of P. melaninogenica was significantly higher in comparison to samples from healthy mucosa [ 164 ]. Based on FISH analyses, epithelial and lamina propria invasion by P. melaninogenica was observed in OLP samples.…”

“…Based on FISH analyses, epithelial and lamina propria invasion by P. melaninogenica was observed in OLP samples. As a novel aspect, P. melaninogenica was confirmed to activate the NF-kB signaling pathway after adhering to and invading macrophages, thus, indicating its role in the pathogenesis of OLP [ 164 ]. In recurrent aphthous stomatitis, Prevotella and Alloprevotella seem to be among the 10 most abundant genera, and the formation of aphthous ulcers may be due to the colonization of Escherichia coli and/or Alloprevotella [ 165 ].…”

Advancing Microbe Detection for Lower Respiratory Tract Infection Diagnosis and Management with Metagenomic Next-Generation Sequencing

“…Infection and Drug Resistance 2023:16 690 infections 28 and oral lichen planus. 29 For another, of the 106 enrolled patients, Tropheryma whipplei was the sole pathogen isolated in 4 patients, comprising 15,428, 4311, 95 and 88 reads respectively, but the symptoms of only 2 patients were consistent with the final clinical diagnosis, the remaining two patients were regarded as positive depending on the criteria for a positive mNGS result but presented discordant clinical signs and symptoms. Tropheryma whipplei is a bacterium associated with Whipple's disease, which commonly demonstrates as weight loss, arthralgia, diarrhea and is diagnosed by the histology of small bowel biopsies.…”