The preventive and therapeutic effect of repetitive transcranial magnetic stimulation on radiation-induced brain injury in mice

Liu, Liyuan; Qin, Tong-Zhou; Guo, Ling; Rong-Rong, Huang; Yu, Jing; Lai, Pan-Pan; Xue, Yi-Zhe; Ding, Gui‐Rong

doi:10.1080/09553002.2022.2038806

Cited by 2 publications

(4 citation statements)

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“…We established the RIBI model as previously described [ 15 ], rTMS intervention was applied during and after radiation process, the details of radiation and rTMS intervention procedure were shown in Fig. 1 A.…”

Section: Resultsmentioning

confidence: 99%

“…Numerous studies believed that multiple factors contributed to RIBI, including hippocampal neurogenesis impairment [ 26 ], increasing neuroinflammatory response [ 27 , 28 ] and oxidative stress, free radical damage theory and BBB disruption [ 29 ] et al BDNF is closely related to the level of neurogenesis and synaptic plasticity, investigating the potential mechanisms of BDNF is of great importance. We previously found that the BDNF expression level significantly increased after rTMS intervention in RIBI mice [ 15 ], while the specific mechanism of rTMS on RIBI has not been well clarified. Hence, in this study, we provided evidence that rTMS improved cognitive function in RIBI mice model through regulating hippocampal neurogenesis and neuroinflammation, and BDNF signaling pathway was participating in it.…”

Section: Discussionmentioning

confidence: 99%

“…SYN and PSD95 are typical markers of synaptic proteins, which are associated with aging and radiation [ 54 ]. We previously found that rTMS could upregulate the expression of PSD95 and SYN in RIBI mice, as well as significantly ameliorate the ultrastructural damage of synapses [ 15 ]. In order to explore the role of BDNF in synaptic plasticity, we blocked the BDNF pathway by K252a and observed the level of synapse-related proteins.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Luo et al recently revealed that high-frequency rTMS significantly improved neural function recovery and promoted neurogenesis in ischemic stroke rat model [ 14 ]. In our previous study, we firstly reported that rTMS had preventive and therapeutic effects on RIBI mice, which were manifested by alleviation of cognitive dysfunction of RIBI mice after rTMS intervention [ 15 ], and BDNF may play a critical role in it. However, the specific role of BDNF in the prevention and treatment of RIBI has not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Repetitive transcranial magnetic stimulation ameliorates cognitive deficits in mice with radiation-induced brain injury by attenuating microglial pyroptosis and promoting neurogenesis via BDNF pathway

Qin,

Guo,

Wang

et al. 2024

Cell Commun Signal

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Background Radiation-induced brain injury (RIBI) is a common and severe complication during radiotherapy for head and neck tumor. Repetitive transcranial magnetic stimulation (rTMS) is a novel and non-invasive method of brain stimulation, which has been applied in various neurological diseases. rTMS has been proved to be effective for treatment of RIBI, while its mechanisms have not been well understood. Methods RIBI mouse model was established by cranial irradiation, K252a was daily injected intraperitoneally to block BDNF pathway. Immunofluorescence staining, immunohistochemistry and western blotting were performed to examine the microglial pyroptosis and hippocampal neurogenesis. Behavioral tests were used to assess the cognitive function and emotionality of mice. Golgi staining was applied to observe the structure of dendritic spine in hippocampus. Results rTMS significantly promoted hippocampal neurogenesis and mitigated neuroinflammation, with ameliorating pyroptosis in microglia, as well as downregulation of the protein expression level of NLRP3 inflammasome and key pyroptosis factor Gasdermin D (GSDMD). BDNF signaling pathway might be involved in it. After blocking BDNF pathway by K252a, a specific BDNF pathway inhibitor, the neuroprotective effect of rTMS was markedly reversed. Evaluated by behavioral tests, the cognitive dysfunction and anxiety-like behavior were found aggravated with the comparison of mice in rTMS intervention group. Moreover, the level of hippocampal neurogenesis was found to be attenuated, the pyroptosis of microglia as well as the levels of GSDMD, NLRP3 inflammasome and IL-1β were upregulated. Conclusion Our study indicated that rTMS notably ameliorated RIBI-induced cognitive disorders, by mitigating pyroptosis in microglia and promoting hippocampal neurogenesis via mediating BDNF pathway.

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Section: Resultsmentioning

confidence: 99%