The prostasome: its secretion and function in man

Ronquist, Gunnar; Brody, Isser

doi:10.1016/0304-4157(85)90008-5

Cited by 370 publications

(339 citation statements)

References 67 publications

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“…15 The semen was initially centrifuged at 10 000 g for 15 min to separate cells from the seminal plasma. The supernatant was then subjected to ultracentrifugation, at 100 000 g for 2 h at 41C.…”

Section: Prostasomesmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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Prostasomes are organelles secreted by prostatic epithelial cells, and are believed to have a role in fertility and prostatic disease. They are known to influence sperm motility and the acrosome reaction, and are thought to have a role in cell transformation, immunosuppression, proliferation, facilitation of local invasion, and angiogenesis. Previously, we have demonstrated the inhibitory effect of prostasomes derived from human semen on angiogenesis using HUVEC cells grown on matrigel. In this study, we use the rat aortic ring assay system, arguably a closer reflection of the in vivo situation. Quantification was by a spectrophotometric method, and underlying mechanisms assessed. Prostasomes demonstrated a clear inhibition of angiogenesis, and this effect persisted after heat treatment of prostasomes to denature protein. This fits with other known effects of prostasomes known to be due to the membrane lipid component, which is unusually high in sphingomyelin and cholesterol.

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Section: Prostasomesmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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“…2 Prostasomes from PC3 and LNCaP cell cultures were obtained by following the conventional procedure with slight modifications. Briefly, cells were recovered at confluence with 0.25% trypsin-EDTA, washed with 30 mM Tris-HCl, 130 mM NaCl, pH 7.6 and stored at À801.…”

Section: Prostasome Preparationmentioning

confidence: 99%

“…The supernatant was then conventionally treated. 2 Protein concentration was determined by the Bio-Rad Protein Assay kit (Bio-Rad Lab, Hercules, CA, USA). Unless otherwise stated, all the reagents were purchased from Sigma (St Louis, MO, USA).…”

Section: Prostasome Preparationmentioning

confidence: 99%

Extracellular matrix degrading enzymes at the prostasome surface

Bellezza¹,

Aisa²,

Palazzo³

et al. 2005

Prostate Cancer Prostatic Dis

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Prostasomes, prostatic secretory vesicles found in human ejaculates, were analyzed to verify the existence at their surfaces of enzymes involved in the degradation of the extracellular matrix. Findings were compared with those of prostasomes isolated from two human adenocarcinoma cell lines that reflect clinical features and molecular pathways of androgen-insensitive and hormone-responsive prostate cancer. Our aim was to determine whether neoplastic transformation is accompanied by changes of glycosidase and protease activities. Our results show that decreases of dipeptidyl peptidase IV and increases of urokinase plasminogen activator and cathepsin B are consistent with the clinical features of the cell lines, whereas increases of glycosidase activities seem to be of scarce biological significance.

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“…For example, EVs offer high physicochemical and biological stability,11 which may be due to the isolating ability of the phospholipid layer, protecting the contents of EVs from exposure to the external environment ( Figure 2 ). 12 As a result, EVs can exist in almost all body fluids, including blood plasma,13 saliva,14 urine,15 bile,16 synovial fluid,17 semen,18 and breast milk 19. By expressing different surface proteins, EVs can have different targeting abilities 20.…”

Section: Introductionmentioning

confidence: 99%

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

2018

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Extracellular vesicles (EVs) are ubiquitous nanosized membrane vesicles consisting of a lipid bilayer enclosing proteins and nucleic acids, which are active in intercellular communications. EVs are increasingly seen as a vital component of many biological functions that were once considered to require the direct participation of stem cells. Consequently, transplantation of EVs is gradually becoming considered an alternative to stem cell transplantation due to their significant advantages, including their relatively low probability of neoplastic transformation and abnormal differentiation. However, as research has progressed, it is realized that EVs derived from native‐source cells may have various shortcomings, which can be corrected by modification and optimization. To date, attempts are made to modify or improve almost all the components of EVs, including the lipid bilayer, proteins, and nucleic acids, launching a new era of modularized EV therapy through the “modular design” of EV components. One high‐yield technique, generating EV mimetic nanovesicles, will help to make industrial production of modularized EVs a reality. These modularized EVs have highly customized “modular design” components related to biological function and targeted delivery and are proposed as a promising approach to achieve personalized and precision medicine.

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The prostasome: its secretion and function in man

Cited by 370 publications

References 67 publications

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Extracellular matrix degrading enzymes at the prostasome surface

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

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