The Protective Effect of Lonicerae flos Extract on Cultured C6 Glioma Cells Damaged by Aluminum of Dementia Inducer

Abstract

This study was performed to evaluate the cytotoxicity of aluminum chloride (AlCl3), and the protective effect of Lonicerae flos (LF) extract on AlCl3-induced cytotoxicity in the cultured C6 glioma cells. Here, the cell viability and antioxidative effects, such as DPPH-radical scavenging activity, superoxide anion scavenging activity, and lipid peroxidation (LP), were assessed. AlCl3 significantly decreased the cell viability in a dose-dependent manner; the XTT50 value was measured at 128.8 M of AlCl3. The cytotoxicity of AlCl3 was determined as highly toxic the y Borenfreund and Puerner's toxic criteria. The butylated hydroxytoluene (BHT) and antioxidant both significantly increased the cell viability, which was damaged by AlCl3-induced cytotoxicity in these cultures. In the protective effect of LF extract on AlCl3-induced cytotoxicity, the LF extract significantly increased the superoxide anion scavenging activity and inhibitory activity of LP, as well as the DPPH-radical scavenging activity. From these results, it is suggested that the oxidative stress may have been involved in the cytotoxicity of AlCl3, and LP extract effectively protected AlCl3-induced cytotoxicity through the antioxidative effects. Conclusively, the natural resources, like LP extract, may be a putative therapeutic agent for the treatment of dementia induced by allergen like aluminum correlated with the oxidative stress.

Alleviating Effects ofEuphorbiae humifusaeL. Extract on the Neurotoxicity Induced by Lead

“…이들의 성분에는 페놀화합물(phenolic compound)을 비롯해 이소프 레노이드(isoprenoids) 및 당배당체(glycoside)와 같은 다양한 물질들이 추출정제되어 있다. 특히, flavonol이나 isoflavones, quercetin, tannin과 같은 성분들은 항산화 작용이나 항염 작용 이 뛰어나 염증이나 세균 증식을 억제하는 중요한 역할을 한다 [12]. 식물 중 지금초(Euphorbiae humifusae L., EH)는 대극과 (Euphorbiaceae)에 속하는 일년생 초본으로 우리나라의 밭이 랑이나 길가 또는 황무지 등에 서식하고 있다.…”