The protective effect of thymoquinone on ethanol-induced acute gastric damage in the rat

Arslan, Seyfullah Oktay; Gelir, Ethem; Armutçu, Ferah; Çoşkun, Ömer; Gürel, Ahmet; Sayan, Hale; Çelik, İsmail

doi:10.1016/j.nutres.2005.06.004

Cited by 78 publications

(49 citation statements)

References 20 publications

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“…The level of IL-1β in TTG group was signifi cantly reduced. In previous studies, it has been reported that the anti-infl ammatory effect of TQ was related to the blockage of the synthesis of leukotrienes and thus to the prevention of tissue infl ammation and oxidative damage (27,28). In our study, TQ showed its anti-infl ammatory effect by strong suppression of IL-1β synthesis.…”

Section: Tab 1 Dna Damage Values Oxidative Stress Parameters and Bsupporting

confidence: 70%

The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine induced acute pancreatitis in rats

Dur¹,

Kose²,

Kocyigit³

et al. 2017

BLL

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The aim of this study is to investigate the potential antioxidant and anti-infl ammatory effects of thymoquinone (TQ) on ceruleine-induced acute pancreatitis. MATERIAL AND METHODS: A total of 14 male Wistar albino rats were divided into 2 groups as follows: (1) normal saline-treated group and (2) thymoquinone-treated groups. For achieving acute pancreatitis, intraperitoneal (IP) cerulein, a stable cholecystokinin (CCK) analogue, was applied in a 50 mcg/kg dose 2 times in onehour interval in total. One hour after last ceruleine injection, IP 2 ml/kg isotonic saline solution was applied to the saline group and IP 5 mg/kg TQ was applied. The rats were sacrifi ced by decapitation 12 h after the last injection of last medication. Blood samples were taken, and serum interleukin-1β (IL-1β), amylase, lipase pancreatic, total antioxidant capacity (TAC), total oxidant status (TOS), and pancreatic Schoenberg scores were determined. Oxidative stress index (OSI) was calculated for each group. Results are given as mean ± SD. A value of p < 0.05 was accepted as statistically signifi cant. SPSS for Windows v15.0 was used for statistical analyses. RESULTS: The increased serum amylase, lipase levels and histopathological scoring of pancreatic tissue showed that acute pancreatitis was present in both groups. Furthermore, serum IL-1β level was signifi cantly reduced in TQ-administered group (p < 0.05). Although serum TAC level was high and TOS level was low, those changes were not statistically signifi cant. Nevertheless, OSI index, which was driven from TOS/TAC, was signifi cantly low in TQ groups (p < 0.05). Although TQ partially ameliorated the acute pancreatitis in terms of histopathological evaluations, the main effect of it was brought about by reducing the hemorrhage in acute pancreatitis (p < 0.05). CONCLUSION: In this study, it was shown that TQ can reduce the infl ammation and has a positive effect on the oxidative status of organism in infl ammatory cases such as acute pancreatitis. This is consistent with partial amelioration of acute pancreatitis in rats given TQ (Tab. 2, Fig. 4, Ref. 31). Text in PDF www.elis.sk.

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Section: Tab 1 Dna Damage Values Oxidative Stress Parameters and Bsupporting

confidence: 70%

The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine induced acute pancreatitis in rats

Dur¹,

Kose²,

Kocyigit³

et al. 2017

BLL

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“…In group 3 (vehicle), 1 h before GU induction, 1 mL CO (10%) was given by orogastric gavage. In group 4 (TQ + GU), 1 h before GU induction, TQ (20 mg/kg) was given by orogastric gavage [14]. In Group 5 (SF + GU), 1 h before GU induction, SF (5 mg/kg) was given by orogastric gavage [6].…”

Section: Experimental Study Designmentioning

confidence: 99%

Gastroprotective effects of sulforaphane and thymoquinone against acetylsalicylic acid–induced gastric ulcer in rats

Zeren

Bayhan

Koçak

et al. 2016

Journal of Surgical Research

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“…Generally, 30% of the seed is fixed oil and 0.40 to 0.45% of volatile oil. Several studies were reported that 18.4 to 24% of volatile oil is thymoquinone [5,6]. There is an emergent interest to study the therapeutic potential of Ns crude extract or pure compound in cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Study of Nigella Sativa and Zingiber zerumbet Extracts, Thymoquinone and Zerumbone Isolated on Human Myeloid Leukemia (HL60) Cell

Norfazlina¹,

Zuraina²,

N.F³

et al. 2014

TOPROCJ

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Abstract:The cytotoxicity effects of Nigella sativa (Ns) and Zingiber zerumbet (Zz) extracts on acute Human myeloid leukemia cell line (HL60) are reported. The HL60 cell line was purchased from the American Tissue Culture Company (ATCC) was treated with petroleum ether (PE) and aqueous (AQ) extracts of Ns and Zz extracts of ethanol (ET) and Hexane (HEX). In an attempt to explain cytotoxicity effects of Ns and Zz extracts by 3-[4,5-dimethylthizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, we examined the effects of Doxorubin (Dox) on their sensitivity as anticancer drugs which was assigns as a positive control in this experiment. Different plant species has different component as bioactive compound. The different activity is present by the different compound. The cytotoxicity effects of plant species are differed based on the presence of their bioactive compound. Therefore, this study was carried out to compare the cytotoxicity effects of Nigella sativa (Ns) and Zingiber zerumbet (Zz) extracts on HL60 cell lines since both of these plants are from different species. Furthermore, this study was also to compare the cytotoxicity effect of plant extract and its compound which are Ns extract and thymoquinone, Zz extract and zerumbone. In this study, Hexane extract of Zz significantly dropped off the percentage of cell viability of HL60 cell by giving the lowest IC 50 value which is 63.72ug/ml (p<0.05). Hexane extracts is more effective solvent compared to ethanol that gave IC 50 value of 345.3ug/ml. Ns extract is less effective on HL60 cell lines that gives the highest IC 50 value which are 654ug/ml for PE and greater than 1000ug/ml for AQ. It is concluded that the cytotoxicity effects of Zz is more proficient as compared to Ns in this cell. HEX extract is more appropriate solvent for the Zz extraction that gave the lowest IC 50 as compared to ET. In addition, PE extract shows the characteristic of more appropriate solvent for Ns extraction compared to AQ solvent since gives the lowest IC 50 value. On the other hand, both of plant species showed apoptosis as mode of cell death.

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The protective effect of thymoquinone on ethanol-induced acute gastric damage in the rat

Cited by 78 publications

References 20 publications

The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine induced acute pancreatitis in rats

The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine induced acute pancreatitis in rats

Gastroprotective effects of sulforaphane and thymoquinone against acetylsalicylic acid–induced gastric ulcer in rats

Cytotoxicity Study of Nigella Sativa and Zingiber zerumbet Extracts, Thymoquinone and Zerumbone Isolated on Human Myeloid Leukemia (HL60) Cell

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