The protective effects of caffeic acid phenethyl ester (CAPE) against liver damage induced by cigarette smoke inhalation in rats

Abstract

The aim of this study was to investigate the histological and biochemical changes in liver of rats exposed to cigarette smoke and effects of caffeic acid phenetyl ester (CAPE) on these changes. For this purpose, 21 male Wistar rats were divided into three groups. Animals in Group I were used as control. Rats in Group II were exposed to cigarette smoke and rats in Group III were exposed to cigarette smoke and injected daily with CAPE. At the end of the 60-days experimental period, all rats were killed by decapitation and blood samples were obtained. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin levels and hepatic superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px ), malondialdehyde (MDA) contents were determined. Following routine histological procedures, liver tissue specimens were examined under a light microscope. The levels of ALT, AST, total bilirubin, SOD, GSH-Px and MDA were significantly increased in rats exposed to cigarette smoke compared with those of the controls. Light microscopic examination of liver specimens from rats exposed to cigarette smoke revealed mononuclear cell infiltration and that some of the hepatocytes had a hyperchromatic nucleus and enlarged sinusoids. The rats which were treated with CAPE along with cigarettes had partially attenuated histological changes associated with cigarette exposure. In conclusion, the damage inflicted by cigarette in the rat liver can be partially prevented by CAPE administration.

Protective antioxidative effects of caffeic acid phenethyl ester (CAPE) in the thyroid and the liver are similar to those caused by melatonin

“…to 12 weeks old female Wistar albino rats (10 μmol/kg body mass; 6-days administration), prevented cisplatin-induced oxidative changes in the liver by reducing ROS production and increasing antioxidative enzyme activities (such as SOD, GSH-Px and CAT) [24]. Caffeic acid phenethyl ester also revealed the protective effects against liver damage induced by cigarette smoking [25], amikacin-induced nephrotoxicity [26] and methotrexate-induced hepatorenal oxidative injury [27]. …”

Neu-164 and Neu-107, two novel antioxidant and anti-myeloperoxidase compounds, inhibit acute cigarette smoke-induced lung inflammation

“…Caffeic acid derivatives are potent antioxidants and have shown some protective effects in CS-exposed rats and rabbits (19,46,55). Caffeic acid is also a potent inhibitor of 5-LO (26), which is upregulated in COPD patients (21,27).…”

Caffeic acid phenethyl ester inhibits liver fibrosis in rats

“…CAPE has been widely accepted as a hepatoprotective folk medicine for many years. Its protective effects are attributed to its cytoprotective, anti-oxidant, antiproliferative, and anti-inflammatory effects, and it has been shown to inhibit both lipoxygenase activity and lipid peroxidation [3][4][5][6][7][8][9] . CAPE can also inhibit phorbol ester-induced H2O2 production and tumor promotion [10,11] , and it was reported that many CAPE activities were related to transcription factor inhibition of nuclear factor-kappa B (NF-κB) [10,[12][13][14] .…”