The protective effects of taurine on hypoxia (performed in the absence of glucose) and on reoxygenation (in the presence of glucose) in guinea-pig heart

Franconi, Flavia; Stendardi, Isabella; Failli, Paola; Matucci, Rosanna; Baccaro, Cecilia; Montorsi, Lucia; Bandinelli, R; Giotti, A

doi:10.1016/0006-2952(85)90556-8

Cited by 55 publications

(9 citation statements)

References 7 publications

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“…Later, Chazov et al (7) (1974) in guinea pig and dog hearts treated with toxic doses of strophathidin-K, Fran coni et al (8) (1985) in Langendorff-perfused guinea pig hearts exposed to the hypoxia-reoxygenation sequence, and Takahashi et al (9) (1988) in cultured embryonic mouse heart cells exposed to high and low [Ca 2+]o also confirmed the antiarrhythmic activities of taurine in con centrations of 1-20 mM.…”

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confidence: 89%

Action Potential Duration-Stabilizing Action of Taurine in Guinea Pig Ventricular Myocytes

Sada

Ban

Sperelakis

1996

Japanese Journal of Pharmacology

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ABSTRACT-To examine taurine actions on the rate of repolarization of action potentials (AP), L-type Ca2+ (Ica), late outward K+ (IK) and the inward rectifier currents as affected by the external Ca2+ concen trations ([Ca2+]o), whole-cell voltage-clamp and current-clamp experiments were conducted in guinea pig ventricular myocytes. At a high (3.6 mM) [Ca 2+]o, 10 mM taurine suppressed both Ica and IK, shortened AP duration and decelerated the rate (-dV/dt) of terminal repolarization of AP. In contrast, at a low (0.9 mM) [Ca2+]o, taurine intensified both Ica and IK, lengthened AP duration and accelerated -dV/dt. However, at either [Ca2+]o, the resting membrane potential was slightly hyperpolarized, and the inward rectifier current examined by the ramp-pulse protocol remained unaffected by taurine. Taurine is suggested to maintain a stable AP duration by altering the inward Ca2+ and IK in the opposite directions, depending on [Ca2+]o. The relevance of the stabilizing action of taurine on the AP duration to its reported anti arrhythmic efficacies is discussed. Keywords:Taurine, Action potential, Ca2+ channel, K+ channel, Antiarrhythmic actionTaurine, 2-aminoethanesulfonic acid (H2NCH2CH2SO3H), is the most abundant amino acid of the amino acid pool of the heart (50%). Although its plasma level is as low as 0.05 to 0.22 mM, the myocardial level is hundreds of times higher than the plasma level: 5.6 (humans) 28 (rats) mM/kg wet weight (1).During the last decade, much evidence for the cardiac effects of taurine was found (2). Sawamura et al. In 1963, Read and Welty (6) first described that taurine, intracellularly given, could prevent the development of epinephrine-induced premature contractions and ceased the arrhythmias of acute or chronic digitalis toxicity. Later, Chazov et al. (7) (1974) in guinea pig and dog hearts treated with toxic doses of strophathidin-K, Fran coni et al. (8) (1985) in Langendorff-perfused guinea pig hearts exposed to the hypoxia-reoxygenation sequence, and Takahashi et al. (9) (1988) in cultured embryonic mouse heart cells exposed to high and low [Ca 2+]o also confirmed the antiarrhythmic activities of taurine in con centrations of 1-20 mM.The action potential (AP) duration is a major deter minant for the electrical refractory period of the cardiac muscle, and the AP duration is ionically controlled by mainly the inward Ca2+ and the delayed rectifier currents. Also, the inward rectifier current plays a key role in maintaining a stable resting potential (10). The present study was designed to test how taurine altered AP parameters, whether the taurine-induced alterations in AP parameters could provide an antiarrhythmic effect and how the AP alterations are related to the changes in the underlying ionic currents. For this purpose, we ex amined taurine-induced changes in ionic currents in the same ionic environment as in AP recordings for a low and high [Ca 2+]o without using any channel-specific blockers.

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confidence: 89%

Action Potential Duration-Stabilizing Action of Taurine in Guinea Pig Ventricular Myocytes

Sada

Ban

Sperelakis

1996

Japanese Journal of Pharmacology

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“…Taurine interacts uniquely with neutral phospholipids of biological membranes, stabilising the cell membrane by altering membrane cation binding characteristics and preventing excessive calcium influxes [12,13]. Exogenous administration of taurine has previously been shown to protect against oxidant-induced lung damage.…”

Section: Discussionmentioning

confidence: 99%

“…One of its main roles in vivo is to protect blood cells and tissues against damage by chlorinated oxidants, particularly HOCl [12][13][14]. Taurine has been shown to provide protection against a variety of toxin-mediated lung injuries, the early stages of which are thought to be mediated by oxygen-free radicals [15,16].…”

Section: Introductionmentioning

confidence: 99%

Taurine Prevents Interleukin-2-Induced Acute Lung Injury in Rats

et al. 2000

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Background: The therapeutic efficacy of interleukin-2 (IL-2) has been limited by a dose-dependent vascular leak syndrome. This may be related to neutrophil-mediated endothelial injury. Taurine has been shown to decrease this injury in vitro. This study investigates the role of taurine in preventing IL-2-induced lung injury, and the role of neutrophil-endothelial interactions in mediating this injury. Methods:Study 1: Sprague-Dawley rats (n = 12/groups) were randomised to controls, IL-2-treated (1 × 10⁶ units), and IL-2-treated with taurine (4% solution, orally for 48 h prior to IL-2 therapy). Lung injury was measured by extravascular lung water (wet/dry weight) and bronchoalveolar lavage protein concentration. Neutrophil infiltration was evaluated by measuring myeloperoxidase activity and bronchoalveolar lavage neutrophil concentration. Study 2: Rats (n = 10/group) were randomised into the same groups as study 1. Neutrophil-endothelial interactions in mesenteric vessels were assessed by intravital microscopy at half-hourly intervals. Results: Taurine reduced IL-2-induced acute lung injury as reflected by a decrease in wet-to-dry lung weight ratio from 7.2 ± 0.5 in the IL-2 group to 4.7 ± 0.3 in the taurine group (p < 0.05), and a decrease in bronchoalveolar neutrophil concentration from 823 ± 19.5 in the IL-2 group to 538 ± 18 in the taurine group (p < 0.05). Intravital microscopy demonstrated that IL-2 increased leucocyte adhesion and migration in mesenteric vessels, and that this was significantly reduced by taurine. Conclusion: These data suggest that taurine prevents IL-2-induced tissue injury in part by decreasing neutrophil-endothelial interactions.

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“…Here, we are attempting to merge molecular imprinting with piezoelectrogravimmetry as transducing element to fabricate a biocompatible MIP sensor for taurine. Taurine is a β ‐amino acid (amino sulfonic acid) significant for functioning of almost all vital organs, to name a few, cardiovascular system liver functioning, reproductive system, retina, muscles, brain, neuroinhibition in central nervous system in addition to osmoregulation in marine invertebrates, energy storage in marine worms, and many more still being reported. Sulfonic acids remain ionized in almost any pH range; thus, taurine is highly soluble in water with low lipophilicity.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical and piezoelectric monitoring of taurine via electropolymerized molecularly imprinted films

Singh

2017

J of Molecular Recognition

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A molecularly imprinted electrochemical quartz crystal microbalance (EQCM) sensor is fabricated here for taurine, a β-amino acid significant for functioning of almost all vital organs. The polymeric film of l-methionine was electrochemically deposited on gold-coated EQCM electrode. Experimental parameters were optimized for controlling the performance of molecularly imprinted polymer (MIP)-modified sensor such as ratio of monomer and template, number of electropolymerization cycles, mass deposited in each cycle, and pH. Thus, fabricated MIP-EQCM sensor was successfully applied for estimation of taurine in solutions with varying matrices, such as aqueous, human blood plasma, milk from cow, buffalo, and milk powder. Under optimized parameters, response of MIP sensor to taurine was linearly proportional to its concentration with limit of detection as 0.12μM. Hence, a highly sensitive and selective piezoelectric sensor for taurine has been reported here via imprinting approach.

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The protective effects of taurine on hypoxia (performed in the absence of glucose) and on reoxygenation (in the presence of glucose) in guinea-pig heart

Cited by 55 publications

References 7 publications

Action Potential Duration-Stabilizing Action of Taurine in Guinea Pig Ventricular Myocytes

Action Potential Duration-Stabilizing Action of Taurine in Guinea Pig Ventricular Myocytes

Taurine Prevents Interleukin-2-Induced Acute Lung Injury in Rats

Electrochemical and piezoelectric monitoring of taurine via electropolymerized molecularly imprinted films

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