The protein expression profile and transcriptome characterization of Pichia caribbica induced by ascorbic acid under the oxidative stress

Yang, Qiya; Diao, Junwei; Legrand, Ngolong Ngea Guillaume; Serwah, Boateng Nana Adwoa; Zhu, Marui; Pang, Bo; Zhang, Hongyin

doi:10.1016/j.biocontrol.2019.104164

Cited by 9 publications

(12 citation statements)

References 24 publications

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“…2-DE and Image Analysis were conducted in accordance with the method delineated by Yang et al and Zhang et al with some modifications [11,13,14]. Isoelectric focusing was used to separate proteins in GE Ettan IPGphor 3, according to the manufacturer's instructions [14].…”

Section: Analysis Of the Differentially Expressed Proteins Of P Expamentioning

confidence: 99%

“…e parameters used for MS search were taxonomy, all series; allowed modifications, carbamidomethyl of cysteine (fixed), oxidation of methionine (variable); and peptide tolerance, ±0.3 Da. Only the highest Mowse score was considered as the most probable identification and was significant (P < 0.05) when protein scores were greater than 88 (NCBInr) or 70 (Swiss-Prot) [14].…”

Section: Identification Of Proteinsmentioning

confidence: 99%

“…Incubated with M. guilliermondii. RNA from the mycelia was extracted in accordance with the prescription of Sangon Co., Shanghai, China, with little modifications [14]. e concentration of RNA was detected by Qubit ® RNA Assay Kit in Qubit ® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA).…”

Section: Transcriptomic Analysis Of P Expansum and P Expansummentioning

confidence: 99%

“…RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system was used (Agilent Technologies, Santa Clara, CA, USA) to assess the integrity of the RNA. e samples that met the experimental requirements were used for the next steps [14].…”

Section: Transcriptomic Analysis Of P Expansum and P Expansummentioning

confidence: 99%

“…Each RNA sample was used to perform RNA-Seq. tRNA-Seq libraries were sequenced on an Illumina HiSeq 2500 platform to generate 125 bp/150 bp single-ended reads [14].…”

Section: Transcriptome Analysismentioning

confidence: 99%

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Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Yang

Dhanasekaran

Apaliya

et al. 2020

Journal of Food Quality

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Antagonistic yeasts can inhibit fungal growth. In our previous research, Meyerozyma guilliermondii, one of the antagonistic yeasts, exhibited antagonistic activity against Penicillium expansum. However, the mechanisms, especially the molecular mechanisms of inhibiting activity of M. guilliermondii, are not clear. In this study, the protein expression profile and transcriptome characterization of P. expansum induced by M. guilliermondii were investigated. In P. expansum induced by M. guilliermondii, 66 proteins were identified as differentially expressed, among them six proteins were upregulated and 60 proteins were downregulated, which were associated with oxidative phosphorylation, ATP synthesis, basal metabolism, and response regulation. Simultaneously, a transcriptomic approach based on RNA-Seq was applied to annotate the genome of P. expansum and then studied the changes of gene expression in P. expansum treated with M. guilliermondii. The results showed that differentially expressed genes such as HEAT, Phosphoesterase, Polyketide synthase, ATPase, and Ras-association were significantly downregulated, in contrast to Cytochromes P450, Phosphatidate cytidylyltransferase, and Glutathione S-transferase, which were significantly upregulated. Interestingly, the downregulated differentially expressed proteins and genes have a corresponding relationship; these results revealed that these proteins and genes were important in the growth of P. expansum treated with M. guilliermondii.

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Section: Analysis Of the Differentially Expressed Proteins Of P Expamentioning

confidence: 99%

Section: Identification Of Proteinsmentioning

confidence: 99%

Section: Transcriptomic Analysis Of P Expansum and P Expansummentioning

confidence: 99%

Section: Transcriptomic Analysis Of P Expansum and P Expansummentioning

confidence: 99%

“…Each RNA sample was used to perform RNA-Seq. tRNA-Seq libraries were sequenced on an Illumina HiSeq 2500 platform to generate 125 bp/150 bp single-ended reads [14].…”

Section: Transcriptome Analysismentioning

confidence: 99%

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Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Yang

Dhanasekaran

Apaliya

et al. 2020

Journal of Food Quality

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Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Dhanasekaran

Yang

Godana

et al. 2021

Pest Management Science

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BACKGROUND In the process of biological control, the antagonistic yeasts contend with various stresses that negatively influence yeasts' biocontrol efficiency. In the current study, we investigated the effect of trehalose supplementation on the biocontrol efficiency and oxidative stress tolerance of Sporidiobolus pararoseus Y16. RESULTS S. pararoseus Y16, an antagonistic yeast cultured in trehalose supplemented medium, exhibited better biocontrol efficiency against Penicillium expansum and Aspergillus tubingensis in table grapes. Trehalose‐treated S. pararoseus Y16 cells showed good proliferation efficiency and oxidative stress tolerance than untreated cells. Increased β‐1,3‐glucanase, catalase, superoxide dismutase activity, and low protein carbonylation were observed in trehalose‐amended S. pararoseus Y16 upon H2O2 exposure. The RNA sequencing results indicated that trehalose significantly altered the transcriptome of S. pararoseus Y16. The GO, KEGG, and COG annotations revealed that the differentially regulated genes corresponded to the various biological process of the yeast. CONCLUSION Our findings suggested that trehalose use could enhance the biocontrol efficiency and oxidative stress tolerance of S. pararoseus Y16. Trehalose supplementation altered the transcriptome of S. pararoseus Y16, particularly the genes that correspond to amino acid metabolism, nucleotide metabolism, and protein modification. Thereby the oxidative stress tolerance and biological control efficiency of S. pararoseus Y16 was enhanced by trehalose. © 2021 Society of Chemical Industry.

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The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus

Zhang¹,

Hao²,

Wu³

et al. 2020

Appl Microbiol Biotechnol

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The protein expression profile and transcriptome characterization of Pichia caribbica induced by ascorbic acid under the oxidative stress

Cited by 9 publications

References 24 publications

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus

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