The proteins encoded by rice grassy stunt virus RNA5 and RNA6 are only distantly related to the corresponding proteins of other members of the genus Tenuivirus.

Abstract

The genome of rice grassy stunt virus (RGSV) consists of six RNA segments. The nucleotide (nt) sequences of the two smallest segments, RNAs 5 and 6, were determined and found to comprise 2704 and 2584 nt, respectively. The 5h-and 3h-terminal sequences of both RNAs were identical over a length of 21 nt and could potentially form a panhandle-like structure due to intramolecular complementarity. Each RNA segment contained a virus (v) sense open reading frame (ORF) in the 5h-proximate region, and a virus complementary (vc) ORF in the 3h-proximate region, indicating an ambisense coding strategy. The protein encoded by the ORF on the vc strand of RNA5 was identified as

Detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers

“…The p5-p5 interaction was reproduced in an Sos recruitment-mediated yeast two-hybrid system as well in by far-Western blots. Native p5 protein extracted from RGSV-infected rice tissue was detected in a large complex with a molecular mass of approximately 260 kDa composed of 12 molecules of p5 or a p5 oligomer with an unidentified host factor(s).The genome of Rice grassy stunt tenuivirus (RGSV), a member in the genus Tenuivirus (12) and an important rice pathogen in East, Southeast, and South Asian countries (8), consists of six ambisense RNA segments containing a total of 12 open reading frames (ORFs) (13,16,17). RGSV replicates and is transmitted by a brown planthopper (Nilaparvata lugens Stal) (9).…”

“…The genome of Rice grassy stunt tenuivirus (RGSV), a member in the genus Tenuivirus (12) and an important rice pathogen in East, Southeast, and South Asian countries (8), consists of six ambisense RNA segments containing a total of 12 open reading frames (ORFs) (13,16,17). RGSV replicates and is transmitted by a brown planthopper (Nilaparvata lugens Stal) (9).…”

Rice grassy stunt virus nonstructural protein p5 serves as a viral suppressor of RNA silencing and interacts with nonstructural protein p3

“…The functions of three RGSV-encoded proteins are known. The protein pC1 (339.1-kDa), encoded on RNA 1 in the viral complementary sense (vcRNA1), may function as an RNA-dependent RNA polymerase (RdRp) and is similar to the RdRp of RSV, with 37 ± 9 % amino acid sequence identity over 2140 amino acid residues [23,24]. The protein pC5 (35.9 kDa), encoded by vcRNA5, is the nucleocapsid protein (N) and accumulates in both RGSVinfected rice and viruliferous brown planthoppers [3].…”

“…RGSV infects rice and induces symptoms including leaf yellowing, stunting, and excess tillering [20,21]. The RGSV genome has six ambisense RNA segments containing 12 open reading frames (ORFs) [18,23]. RNAs 1, 2, 5 and 6 of RGSV correspond to RNAs 1, 2, 3 and 4, respectively, of rice stripe virus (RSV), the type member of the genus Tenuivirus [3].…”

“…The functions of some other RGSV proteins can be deduced by comparison with their counterparts in RSV. However, functional verification is essential, given the low amino acid sequence identity between these proteins [3,23,24]. Protein-protein interactions (PPIs) play a crucial role in many cellular processes.…”