The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

Lai, Wei-Kuang; Wu, Yang‐Chang; Yeh, Tai-Sheng; Hsieh, Chun-Ren; Tsai, Yi‐Hong; Wei, Chien-Kei; Li, Chi‐Ying; Lu, Ying‐Chen; Chang, Fang‐Rong

doi:10.1016/j.fshw.2022.04.032

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…Recent studies have shown that the cytoplasm can affect colony morphology, the mycelial growth rate, enzyme activity, the agronomic traits of fruiting bodies and polysaccharide and triterpene content [28,42,44,45], consistent with our results. The contents of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharides and total triterpenes of the fusants obtained using protoplast fusion of A. cinnamomea and C. militaris were significantly increased [34]. These results showed that the use of protoplast fusion technology to recombine the nucleus and cytoplasm of parents can significantly increase the content of active substances [46,47].…”

Section: Discussionmentioning

confidence: 78%

“…Biparental inactivation is a commonly used method in protoplast fusion breeding, and the technology is well established and has been applied in the breeding of several new strains of edible fungi [33][34][35]. Excessive inactivation will seriously affect the cell structure of protoplasts and affect their regeneration [36].…”

Section: Discussionmentioning

confidence: 99%

“…The protoplasts (2 × 10 7 ) of Antrodia cinnamomea and C. militaris could be completely thermally inactivated at 55 • C for 10 min. The protoplasts of A. cinnamomea and C. militaris could be completely inactivated by 10 cm irradiation with a 30 W UV lamp for 7 min [34]. Therefore, the sensitivity of protoplasts of different species to inactivation conditions is different, and the number of protoplasts, inactivation temperature and inactivation time should be controlled during thermal inactivation.…”

Section: Discussionmentioning

confidence: 99%

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Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Li,

Liu,

et al. 2023

Horticulturae

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Ganoderma lingzhi is an important medicinal fungus, and it is particularly important to select strains with high yields and active substance contents. In this study, protoplasts of G. lingzhi were thermally inactivated to destroy intracellular enzyme proteins and preserve DNA. The DNA of G. resinaceum was damaged by ultraviolet (UV) radiation, and other components of the protoplasm except DNA were preserved. Then, the protoplast was induced using polyethylene glycol (PEG) for fusion. The results showed that the optimal thermal inactivation conditions for G. lingzhi were 30 min in a 45 °C water bath, and the optimal UV inactivation conditions for G. resinaceum were 70 s of irradiation using a 20 W UV lamp at a vertical distance of 15 cm. Antagonistic tests, internal transcribed space (ITS) and mitochondrial DNA identification, intersimple sequence repeat (ISSR) molecular markers and morphology were used to distinguish the parents from the fusants. Four true fusants were obtained, and the yield was 2.5%. The fruiting body yield of the fusants was significantly higher than that of G. lingzhi, and the polysaccharide and triterpene contents of the RAD-64 fusant were significantly higher than those of G. lingzhi. The results presented in this paper show that protoplast fusion technology can effectively improve G. lingzhi varieties and support the breeding of new varieties.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Li,

Liu,

et al. 2023

Horticulturae

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“…Lai et al [52] obtained new strains with high production of cordycepin and cordycepic acid by fusing protoplasts of Antrodia cinnamomea and Cordyceps militaris; Peng et al [53] conducted protoplast fusion involving two Ganoderma lucidum protoplasts and obtained a new strain of Ganoderma with high production of polysaccharides and triterpenes. From the above research, it is clear that protoplast fusion is a common breeding tool within the field of Ganoderma breeding.…”

Section: Discussionmentioning

confidence: 99%

Ganoderma Fusions with High Yield of Ergothioneine and Comparative Analysis of Its Genomics

Xie,

Yu,

You

et al. 2023

JoF

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Ergothioneine (EGT), an exceptional antioxidant found ubiquitously across diverse living organisms, plays a pivotal role in various vital physiological regulatory functions. Its principal natural sources are mushrooms and animal liver tissues. Ganoderma spp., a traditional Chinese food and medicinal mushroom, boasts high concentrations of EGT. To advance the development of novel Ganoderma spp. strains with enhanced EGT yields, we employed an efficient Ganoderma spp. protoplasmic fusion system. Through molecular and biological characterization, we successfully generated seven novel fusion strains. Notably, fusion strain RS7 demonstrated a remarkable increase in mycelial EGT production (12.70 ± 1.85 mg/L), surpassing the parental strains FQ16 and FQ23 by 34.23% and 39.10%, respectively. Furthermore, in the context of the fruiting body, fusion strain RS11 displayed a notable 53.58% enhancement in EGT production (11.24 ± 1.96 mg/L) compared to its parental strains. Genomic analysis of the RS7, the strain with the highest levels of mycelial EGT production, revealed mutations in the gene EVM0005141 associated with EGT metabolism. These mutations led to a reduction in non-productive shunts, subsequently redirecting more substrate towards the EGT synthesis pathway. This redirection significantly boosted EGT production in the RS7 strain. The insights gained from this study provide valuable guidance for the commercial-scale production of EGT and the selective breeding of Ganoderma spp. strains.

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GC–MS Analysis of Polysaccharides from an Intergeneric Hybrid of Pleurotus florida and Cordyceps militaris: A Comparative Study

Meshram,

Thakur,

Jadhav

et al. 2024

Appl Biochem Biotechnol

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The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

Cited by 6 publications

References 16 publications

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Ganoderma Fusions with High Yield of Ergothioneine and Comparative Analysis of Its Genomics

GC–MS Analysis of Polysaccharides from an Intergeneric Hybrid of Pleurotus florida and Cordyceps militaris: A Comparative Study

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