The Pterocellins, Novel Bioactive Alkaloids from the Marine Bryozoan Pterocella vesiculosa

Yao, Biao; Prinsep, Michèle R.; Nicholson, Brian K.; Gordon, Dennis P.

doi:10.1021/np030104y

Cited by 32 publications

(39 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hollow fiber assay has been incorporated into the drug discovery programs of many laboratories around the world including Argentina,69 Austria,70 France,71 Germany,72 India,73Italy,74–78 New Zealand,79 Poland,80,81 Spain,82,83 Sweden,84 and the United Kingdom 85–89…”

Section: Discussionmentioning

confidence: 99%

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

Pezzuto

Farnsworth

et al. 2009

J. Nat. Prod.

View full text Add to dashboard Cite

The in vivo hollow fiber assay was developed at the National Cancer Institute (NCI) to help bridge the gap between in vitro cell-based assays and human tumor models propagated in immunodeficient mice. The goal was to develop an intermediate assay that could help predict which compounds found active in the 60-cell line panel would be active in a subsequent xenograft system. This was necessary due to the high cost of the traditional xenograft assay in terms of number of animals required, time for assay completion, and financial commitment necessary. To address this problem, investigators of the NCI Developmental Therapeutics Program designed a method of propagating human cancer cells in inert hollow fibers with pores small enough to retain the cancer cells but large enough to permit entry of potential chemotherapeutic drugs, including large proteins and other important substances. Fibers containing proliferating cancer cells are transplanted into the peritoneum or under the skin, the host mice are treated with a test agent and the fibers are subsequently retrieved for analysis of viable cell mass. The assay has been successful in helping investigators from around the world, including our own research group, prioritize compounds active in vitro for further testing in the traditional xenograft system.

show abstract

Section: Discussionmentioning

confidence: 99%

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

Pezzuto

Farnsworth

et al. 2009

J. Nat. Prod.

View full text Add to dashboard Cite

show abstract

“…Structures of pterocellins A–F (Fig. 1 ) have been elucidated by NMR spectroscopy and single-crystal X-ray diffraction (Prinsep 2008 ; Yao et al 2003 ). Bioactivity testing of two pterocellins (A, B) indicated promising anti-bacterial, anti-tumour, and anti-fungal activities.…”

Section: Introductionmentioning

confidence: 99%

“…Bioactivity testing of two pterocellins (A, B) indicated promising anti-bacterial, anti-tumour, and anti-fungal activities. It was found that pterocellins A and B were active against the P388 murine leukaemia cell line (IC50 values 477 and 323 ng/mL, respectively); non-small cell lung (NCI-H23), melanoma (MALME-3M, M14, SK-MEL-5), and breast (MDA-MB-435, MDA-N) cell lines (Yao et al 2003 ). However, the mode of cytotoxicity of pterocellin A is not yet known.…”

Section: Introductionmentioning

confidence: 99%

Pterocellin A isolated from marine bryozoan Pterocella vesiculosa is cytotoxic to human HeLa cells via mitochondrial apoptotic processes

2016

Self Cite

View full text Add to dashboard Cite

Pterocellin A is a novel bioactive alkaloid isolated from the New Zealand marine bryozoan Pterocella vesiculosa. It exhibits potent antitumour activity towards the P388 (murine leukaemia) cell line in vitro and is selectively sensitive towards certain non-small cell lung, melanoma, and breast cancer cell lines, however, the biological mode of action of pterocellin A is unknown. Using the human cervical cancer cell line HeLa, we show that pterocellin A exhibited cytotoxicity against HeLa cells with an IC50 of 886 ng/mL. Time-course MTT and LDH assays were carried out and the results showed only a low level of cytosolic LDH was detected in the supernatant after all the cells have died from pterocellin A treatment at 2000 ng/mL. This indicated the cells maintained membrane integrity upon cell death which suggested apoptotic cell death. Additionally, morphological changes were observed under the microscope after 6 h of treatment. Cell shrinkage and nucleus condensation were observed, as well as apparent membrane blebbing, a key feature of apoptosis. The MTT data was also indicative of mitochondria impairment which could suggest that pterocellin A targets the mitochondria. This idea was supported by the observed changes in the morphology and location of the mitochondria after exposure to pterocellin A. Furthermore, the level of activated caspase-3 in HeLa cells increased after treatment with pterocellin A; activated caspase-3 can only be detected after a series of signalling events following the induction of apoptosis. These data support the notion that pterocellin A is an inducer of apoptosis in HeLa cells possibly via mitochondria related processes.

show abstract

“…4,8 Natural products from bryozoans have been of interest due to uniqueness of their structures and their biological and ecological activities. 9 Ecological roles of secondary metabolites from bryozoans have been demonstrated on several occasions. Crude extracts from the bryozoans Amathia wilsoni and Orthoscuticella ventricosa acted as chemical defenses against feeding by the fish Acanthaluteres sp., 10 and also exhibited strong anti-bacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Chemical Defense and Chemical Diversity in the Exotic Bryozoan Amathia verticillata

Santos¹,

Clavico²,

Parra³

et al. 2016

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

The present investigation tests the effects against feeding by fishes of crude extracts obtained from eleven distinct populations of the bryozoan Amathia verticillata, an invasive species found globally in tropical to warm-temperate waters. Investigation of extracts from 11 populations of A. verticillata led to the identification and quantification of the known indole alkaloid 2,5,6-tribromo-N-methylgramine and isolation and identification of the new indole alkaloid, 2,6-dibromo-N-methylgramine. One extract of A. verticillata from Brazil significantly deterred feeding, while other extracts of A. verticillata from Florida significantly stimulated feeding by fishes, in field assays performed in Brazil. The same extracts of Florida samples showed variable effects on feeding, ranging from attraction to deterrence, in assays carried out in Florida. The absence of broad chemical defenses against feeding by fish suggests that the establishment of A. verticillata as an invasive species into new areas may be due to reasons other than defensive chemistry.

show abstract

The Pterocellins, Novel Bioactive Alkaloids from the Marine Bryozoan Pterocella vesiculosa

Cited by 32 publications

References 16 publications

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

Pterocellin A isolated from marine bryozoan Pterocella vesiculosa is cytotoxic to human HeLa cells via mitochondrial apoptotic processes

Evaluation of Chemical Defense and Chemical Diversity in the Exotic Bryozoan Amathia verticillata

Contact Info

Product

Resources

About