The qid74 gene from Trichoderma harzianum has a role in root architecture and plant biofertilization

Abstract

The Trichoderma harzianum qid74 gene encodes a cysteine-rich cell wall protein that has an important role in adherence to hydrophobic surfaces and cellular protection; this gene was upregulated in Trichoderma high-density oligonucleotide (HDO) microarrays in interaction with tomato roots. Using a collection of qid74-overexpressing and disrupted mutants the role of this gene in cucumber and tomato root architecture was analysed in hydroponic and soil systems under greenhouse conditions. No significant differences were found in the pattern of root colonization and the length of primary roots of cucumber or tomato plants inoculated by T. harzianum CECT 2413 wild-type (wt) strain or any of the qid74 transformants. However, compared to the wt treatment, lateral roots were significantly longer in plants inoculated with the overexpressing transformants, and shorter in those treated with the disruptant strains. Microscopic observations revealed more and longer secondary root hairs in cucumber plants treated with the qid74-overexpressing mutants and fewer and shorter hairs in roots treated with qid74-disrupted transformants, compared to those observed in plants inoculated with the wt strain. qid74-induced modifications in root architecture increased the total absorptive surface, facilitating nutrient uptake and translocation of nutrients in the shoots, resulting in increased plant biomass through an efficient use of NPK and micronutrients.

Management of cauliflower seedling disease (Sclerotium rolfsii) in seedbed with different substrate based Trichoderma harzianum Bio-fungicides

“…The promotion of plant growth in terms of length and weight of shoot and root, due to use of Trichoderma spp. as soil amendment was documenetd by several investigators (Chang et al, 1986;Azarmi et al, 2011;Harman et al, 2012;Hermosa et al, 2012;Samolski et al, 2012). Enhancing root growth of cauliflower seedling in S. rolfsii sick soils was observed in the present experiment which was supported by the findings of John et al (2010).…”

Effect of substrates to formulate Trichoderma harzianum based bio-fungicide in controlling seedling disease (Rhizoctonia solani) of brinjal

“…The growth promotion in plant with special reference to the length and weight of their shoots and roots was enhanced due to Trichoderma spp. soil amendment (Samolski et al, 2012;Harman et al, 2012;Hermosa et al, 2012). Similarly, enhanced root length and root weight of many plants were also reported by the application of Trichoderma spp.…”

Pengaruh Waktu Aplikasi dan Jenis Trichoderma terhadap Penyakit Jamur Akar Putih pada Bibit Tanaman Karet

“…Di samping dapat mengeluarkan metabolit sekunder (Mukherjee, Horwitz, Herrera-Estrella, Schmoll, & Kenerley, 2013;Vinale et al, 2014), beberapa jenis Trichoderma juga dapat berperan sebagai dekomposer untuk meningkatkan kesuburan tanah sehingga dapat memicu pertumbuhan tanaman (Kaewchai, Soytong, & Hyde, 2009;Contreras-Cornejo, Machias-Rodriguez, Cortẻs-Penagos, & Lỏpez-Bucio, 2009;Viterbo, Landau, Kim, Chernin, & Chet, 2010;Akladious & Abbas, 2012;Haque, Ilias, & Molla, 2012;Hermosa, Viterbo, Chet, & Lorito, 2012;Samolski, Rincỏn, Pinzỏn, Viterbo, & Monte, 2012;Promwee, Issarakraisila, Intana, Chamswarng, & Yenjit, 2014).…”