The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering

Weder, Gilles; Guillaume‐Gentil, Orane; Matthey, Nadège; Montagne, Franck; Heinzelmann, H.; Vörös, János; Liley, Martha

doi:10.1016/j.biomaterials.2010.04.068

Cited by 40 publications

(33 citation statements)

References 37 publications

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“…Therefore, in the traditional single cell force spectroscopy, the cell contact time with the substrate cannot be more than 1 hour. Although Weder et al succeeded to make the cell interacts specifically with the cantilever through a fibronectin-modification to enhance the binding strength of the cantilever to the cell, extending the cell-to-substrate contact time to 24 hours, the adhesion of cells to protein-modified cantilever beams takes 30 minutes or more [10]. In order to interact specifically with fibronectin, the cells will alter their gene expression to diffuse the adhesion receptor in the membrane surface, and altering the structure of the cell surface.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Cell Adhesion Force with Atomic Force Microscope

Chen¹,

Xie²

2017

Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)

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Cell adhesion to cells or substrates is the basis for cell colonization and survival. Based on atomic force microscope (AFM), a single probe was used to quantitatively measure the adhesion of different cell types. The self-made pneumatic control system produces negative pressure to fix the cells, replacing the traditional chemical fixation method, reducing the fixation time from about 30 min to a few seconds, and a single probe can test about 15 cells, greatly improving single-cell force spectroscopy test speed. As a result, statistical data can be quickly recorded, the range of cells tested can be expanded, and the physiological activity of the cells during the test is preserved. In this paper, we studied the adhesion of three different mammalian cells to polystyrene substrates, and indicated that the adhesion forces depend on the probe retraction speed and the contact time: the adhesion forces are proportional to the logarithm of the probe retraction speed and increase with increasing contact time.

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Section: Discussionmentioning

confidence: 99%

Quantification of Cell Adhesion Force with Atomic Force Microscope

Chen¹,

Xie²

2017

Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)

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“…However, this study did not discuss the related indexes of the scaffold. In subsequent studies, we will assess new scaffold materials, electrostatic spinning (Agarwal et al, 2008;Janjanin et al, 2008;Wright et al, 2010), and cell-chip technology (Kaneshiro et al, 2006;Mitani et al, 2009;Tani et al, 2010;Weder et al, 2010), and in more detail compare the properties of the engineered cartilages with normal cartilages.…”

Section: Discussionmentioning

confidence: 99%

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

Wu¹,

Cui²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed at investigating the ability of cartilagederived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lacticco-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRNA and protein expressions of hCDMP1 were detected by reverse transcriptase-polymerase chain reaction and Western blotting. The expression of type II collagen (Col II) and glycosaminoglycan (GAG) were detected by immunohistochemistry. The cytoskeletal culture systems before and after transfection were transplanted into the rabbit full-thickness defects of thyroid cartilage for observation of the repair of cartilage defects from general and histological aspects. The exogenous hCDMP1 gene could be successfully transplanted into BMSCs through adenovirus infection to obtain a stable expression. Compared with the control group, hCDMP1 gene-transfected BMSCs had enhanced secretory abilities of Col II, GAG, and other cartilagespecific matrices, with a trend of promoting cartilage differentiation. The transfected cytoskeletal complexes could more effectively repair laryngeal cartilage defects. hCDMP1 gene-transfected BMSCs/PLGA 3-D biological scaffold compounds transplanted into animal bodies could effectively repair laryngeal cartilage defects.

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“…Several reports are available on apatite formation ability of CNT-surface, when immersed in simulated body fluid [124][125][126][127][128][129]. Still, a thorough investigation of the biocompatibility of HA-CNT composites, prepared by different techniques, is mandatory for the clinical application.…”

Section: Figure 225: Sem Images Showing the Adhesion Of Fibroblast Omentioning

confidence: 99%

“…Studies by different research groups have found CNT surfaces suitable for apatite precipitation [124][125][126]. Akasaka et al have reported a 14 days incubation period for apatite precipitation on CNT surface when immersed in standard SBF [126].…”

Section: Osteoblast/fibroblast Proliferation and Viabilitymentioning

confidence: 99%

“…Atomic force microscopy (AFM), micropipette suction, cytodetacher and shearing by a cantilever are the major techniques in this category. Although AFM is a suitable technique for measuring the adhesion of a single cell [125], the attachment of a cell on the AFM probe or cantilever can pose stress induced damage to the cell due to the long time required for the procedure. Micropipette suction can also measure the force required to detach a cell from the substrate, but inherits a risk of rupturing the cell membrane and damaging the cell due to application of localized tensile force [126].…”

Section: Adhesion Quantification Techniques For a Single Cellmentioning

confidence: 99%

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Hydroxyapatite-Nanotube Composites and Coatings for Orthopedic Applications

Lahiri¹

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The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering

Cited by 40 publications

References 37 publications

Quantification of Cell Adhesion Force with Atomic Force Microscope

Quantification of Cell Adhesion Force with Atomic Force Microscope

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

Hydroxyapatite-Nanotube Composites and Coatings for Orthopedic Applications

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