The Rab GTPase activating protein Gyp2 contributes to UV stress tolerance in Metarhizium acridum

Abstract

GTPase activation protein (GAP) for Rab GTPases can accelerate GTP hydrolysis to alter the activity of Rab GTPases. To explore the function of GAP in entomopathogenic fungi, we constructed a deletion mutant of Gyp2 gene, a member of the Gyp (GAP for Ypt/Rab proteins) family in the locust-specific fungal pathogen, Metarhizium acridum. Results showed that the ∆MaGyp2 mutant had dramatically decreased tolerance to ultraviolet irradiation compared to wild type strain. Quantitative real-time PCR revealed that UV irradiation repair related genes Uve1 and WC1 were downregulated in ∆MaGyp2 mutant. Seven of other ten Gyp family members had significantly increased transcription in ∆MaGyp2 mutant compared with wild type, which may partly rescue the deficiency of MaGyp2.

AtGAP1 Promotes the Resistance to Pseudomonas syringae pv. tomato DC3000 by Regulating Cell-Wall Thickness and Stomatal Aperture in Arabidopsis

“…GAPs stimulate the GTPase activity of G-proteins by converting them between the active GTP-bound form and inactive GDP-bound form [ 1 , 2 ]. GAPs are involved in various stress responses in plants, fungi, insects, and mammals [ 3 , 4 , 5 , 6 , 7 , 8 ]. G-proteins have been suggested as the molecular switch that regulates a wide variety of plant processes such as defense [ 9 , 10 , 11 ], growth and development [ 12 , 13 ], and phytohormone responses [ 14 , 15 ].…”