The rabbit antibody response to immunization with Haemophilus ducreyi A comparative study for immunoglobulin detection using diffusion-in-gel enzyme-linked immunosorbent assay, countercurrent immunoelectrophoresis, and double-diffusion-in-gel

Abstract

Haemophilus ducreyi was used for immunization of rabbits. The serum level of specific IgG, IgM, and IgA antibodies to the same agent was determined by diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) and precipitating antibodies were detected with countercurrent immunoelectrophoresis (CIE) and double-diffusion-in-gel (DD) techniques. Antibodies to H. ducreyi could be detected in all rabbits within two weeks with all three techniques used.