The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

A histone deacetylase corepressor complex regulates the Notch signal transduction pathway

“…In the presence of Notch it functions as a transcriptional activator, whereas in the absence of Notch it represses transcription (Hsieh and Hayward 1995). The promoter of the adenovirus pIX polypeptide contains a consensus binding site for CBF1/RBP-J that mediates repression both in vitro and in vivo (Dou et al 1994). A similar repressive function has been ascribed to CBF1/RPB-J binding sites in the IL-6 and NF-B2 promoters (Kannabiran et al 1997;Plaisance et al 1997;Miyazawa et al 1998).…”

The Notch signaling pathway: Transcriptional regulation at Notch target genes

“…NICD cannot bind directly to DNA but heterodimerizes with the DNA binding protein RBP-J (recombination signal sequence-binding protein Jk, also called CSL, CBF1, Su(H) and LAG-1) and activates transcription of genes containing RBP-J binding sites. Interestingly, RBP-J was originally identified as a repressor of transcription by Vales and colleagues [5]. When studying the promoter region of the adenovirus pIX gene, a 10bp repressive element was characterized and it was shown that RBP-J is the factor that binds to this element.…”

“…Other viruses that have interactions with the Notch pathway are adenovirus, HPV and simian virus 40 (SV40), reviewed [173]. The first association of adenovirus came from the recognition of a RBP-J binding site in the pIX promoter [5]. Subsequently, adenovirus 13SE1A was shown to bind to RBP-J and to be capable of activating promoters containing RBP-J binding sites [174].…”

Neoplastic transformation by Notch is independent of transcriptional activation by RBP-J signalling

“…RBP-J represses transcription by binding to speci®c sequence motifs in the promoter of genes and recruits corepressors like SMRT or CIR (Dou et al, 1994;Kao et al, 1998;Hsieh et al, 1999). The Notch-IC/RBP-J interaction abolishes the repressor function of RBP-J and activates transcription.…”