The regular supplementation with an antioxidant mixture decreases oxidative stress in healthy humans. Gender effect

Actis‐Goretta, Lucas; Carrasquedo, Fernando; Fraga, César G.

doi:10.1016/j.cccn.2004.06.009

Cited by 30 publications

(21 citation statements)

References 29 publications

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“…Vitamin E (as α-tocopherol) and ubiquinone (as ubiquinol-10) were determined by HPLC with electrochemical detection [29,30]. Briefly, 100 μL-aliquots of tissue homogenate were mixed with 500 μL of methanol, vortexed for 30 s, added with 4 mL of hexane, vortexed for 1 min, and centrifuged for 5 min at 1000 ×g to separate the organic phase.…”

Section: Antioxidantsmentioning

confidence: 99%

Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Milei¹,

Forcada²,

Fraga

et al. 2007

Cardiovasc Res

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Section: Antioxidantsmentioning

confidence: 99%

Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Milei¹,

Forcada²,

Fraga

et al. 2007

Cardiovasc Res

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“…Base-line oxidation is greater in males than in females (22). Estrogen has antioxidant properties at pharmacological concentrations, whereas testosterone does not (23).…”

mentioning

confidence: 94%

Orchiectomy Attenuates Post-ischemic Oxidative Stress and Ischemia/Reperfusion Injury in Mice

Kim

Kil

Seok

et al. 2006

Journal of Biological Chemistry

115

116

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Males are much more susceptible to ischemia/reperfusion (I/R)-induced kidney injury when compared with females. Recently we reported that the presence of testosterone, rather than the absence of estrogen, plays a critical role in gender differences in kidney susceptibility to I/R injury in mice. Although reactive oxygen species and antioxidant defenses have been implicated in I/R injury, their roles remain to be defined. Here we report that the orchiectomized animal had significantly less lipid peroxidation and lower hydrogen peroxide levels in the kidney 4 and 24 h after 30 min of bilateral renal ischemia when compared with intact or dihydrotestosterone-treated orchiectomized males. The post-ischemic kidney expression and activity of manganese superoxide dismutase (MnSOD) in orchiectomized mice was much greater than in intact or dihydrotestosterone-administered orchiectomized mice. Four hours after 30 min of bilateral ischemia, superoxide formation was significantly lower in orchiectomized mice than in intact mice. In Madin-Darby canine kidney cells, a kidney epithelial cell line, 1 mM H 2 O 2 decreased MnSOD activity, an effect that was potentiated by pretreatment with dihydrotestosterone. Orchiectomy prevented the post-ischemic decrease of catalase activity. Treatment of male mice with manganese(III) tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP), a SOD mimetic, reduced the post-ischemic increase of plasma creatinine, lipid peroxidation, and tissue hydrogen peroxide. These results suggest that orchiectomy accelerates the post-ischemic activation of MnSOD and reduces reactive oxygen species and lipid peroxidation, resulting in reduced kidney susceptibility to I/R injury.Gender differences in disease susceptibility have been well characterized in many cardiovascular diseases (1-3). The increased risk for cardiovascular diseases in men and postmenopausal women (4) has been attributed primarily to lack of estrogen-mediated protection (5-7). Recently we demonstrated that male mice are much more susceptible to kidney ischemia/reperfusion (I/R) 5 injury when compared with female mice (8), and that orchiectomy decreases kidney susceptibility to I/R injury (9), whereas ovariectomy has no effect on kidney susceptibility to I/R injury (8). Testosterone administration to orchiectomized male or female mice reverses the protective phenotype, increasing susceptibility to kidney I/R injury. We concluded that the presence of testosterone, rather than the absence of estrogen, plays a critical role in the gender differences in kidney susceptibility to I/R injury. The detailed molecular mechanisms responsible for this testosterone effect remain to be defined.Ischemia/reperfusion markedly increases the production of reactive oxygen species (ROS) including superoxide anions, hydroxyl radicals, hypochlorous acid, hydrogen peroxide, and peroxynitrite (10). The abnormal excessive production of ROS results in lipid peroxidation, leukocyte activation, endothelial cell damage, and cytokine production, all of which contribute to tissue ...

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“…Gender differences in the effect of Al on the kidney and liver have received too little attention such as: (1) sexual differences in the oxidant-antioxidant systems [50,51], (2) baseline oxidation is greater in males than in females [52] and (3) oestrogens have antioxidant properties at pharmacological concentrations, whereas testosterone does not [53]. Male sex hormones have been linked with the progression of renal injury [54,55], and female sex hormones have been postulated to be renal protective [56][57][58].…”

mentioning

confidence: 99%

Melatonin Prevents Oxidative Stress in Ovariectomized Rats Treated with Aluminium

Contini

Millen

González

et al. 2011

Biol Trace Elem Res

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This study is designed to determine the simultaneous effect of aluminium (Al) and melatonin (Mel) treatment in intact and ovariectomized (Ovx) female rats on oxidative stress and their inter-organ relationship in the kidney and liver. Al-treated rats received an intra-peritoneal injection of solution of aluminium lactate (0.575 mg Al/100 g of body weight, three times a week), during 12 weeks. Mel groups received intra-peritoneal injections of melatonin at a dose of 10 mg/kg/day, 5 days/week, during 12 weeks. The results of this study showed that Al treatment in female rats modifies homeostasis of glutathione and the antioxidant capacity of the rat liver and kidney. The alteration of glutathione homeostasis and oxidative status was not associated with an increased lipid peroxidation in both organs with the exception of the increase observed in the liver of Ovx rats. Al also induced modifications in the activity of some enzymes related to the glutathione cycle: GSH-Px in the liver and kidney and glutathione reductase only in the kidney. Al exposure decreased CAT activity in both the kidney and liver of intact and Ovx groups. The administration of Mel in the intact and castrated females treated with Al seems to reduce oxidative changes in the liver and kidney of intact and Ovx rats.

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The regular supplementation with an antioxidant mixture decreases oxidative stress in healthy humans. Gender effect

Cited by 30 publications

References 29 publications

Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Orchiectomy Attenuates Post-ischemic Oxidative Stress and Ischemia/Reperfusion Injury in Mice

Melatonin Prevents Oxidative Stress in Ovariectomized Rats Treated with Aluminium

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