The Regulatory Role of Macrophages in Antigenic Stimulation Part Two: Symbiotic Relationship between Lymphocytes and Macrophages

Unanue, Emil R.

doi:10.1016/s0065-2776(08)60919-0

Cited by 465 publications

(105 citation statements)

References 547 publications

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“…These data are supported by previous data from us (10,34,36) and others (19). Decreased Ia expression on TBH M+ limits their ability to act as antigen-presenting cells (26,27) and accessory cells in Ia-dependent reactions, such as the AMLR (20,221. In the spleen, the number and percentage of Mac-1 + 2 + , Mac-lf3+, and Mac-2+3+ Mi$ significantly increased during tumor growth. The increase in Mac-1 + M+ in the TBH follows a shift in the phenotype of the splenic accessory M+ from Mac-1-in the normal host to Mac-l+ in the TBH (9).…”

Section: Dual Antigen Expression On Splenic M+supporting

confidence: 85%

“…M+ presentation to antigenspecific and autoreactive T cells depends on la expression (20,22,26,27,35,36), so changes in Ia expression would play a vital role in decreased MHC-restricted immune reactivity during tumor growth. Because M+ with the Ia-phenotype are associated with suppressor activity (3), a two-fold increase in TBH Ia-M+ plus the four-to five-fold increase in TBH splenic M4 could explain the increased suppressor activity seen in TBH M+.…”

Section: Dual Antigen Expression On Splenic M+mentioning

confidence: 99%

“…Mac-3 is found on M 4 and is a differentiation antigen with variable expression (14,29). Ia antigen is the class I1 major histocompatibility complex marker and is needed for effective M+ antigen presentation to T cells (26,27). In this study, we investigated the dual expression of M$ surface markers by flow cytometry.…”

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confidence: 99%

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Two‐color flow cytometric analysis of the expression of MAC and MHC Class II antigens on macrophages during tumor growth

1990

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Tumor-bearing host (TBH) macrophages (M+) exhibit immune dysfunction that is concomitant with phenotypic changes. We examined M+ subpopulations by changes in the expression of surface antigens Mac-1, -2, -3, and Ia on normal and TBH peritoneal and splenic M+. M+ were double-labeled and analyzed by flow cytometry to observe multiple expression of surface antigens. Tumor growth alters the multiple expression of these M+ markers. Peritoneal and splenic M+ had different Mac+ and Mac+Ia+ population percentages. In TBH, peritoneal M+ had decreased percentages of Mac-1+2+, Mac-1+3+, Mac-2+3+, and Mac+Ia+ M+. This decrease correlated with functional changes in TBH M+. In contrast, there was an increase in Mac-2-Ia-TBH peritoneal M+. Previously undiscovered Mac-1+2-3-and Mac-1-2-3+ populations were found. In contrast to peritoneal M+, there was an increase in the percentage of Mac-l+2+, Mac-1+3+, and Mac-2+3+ splenic TBH M+ but, like peritoneal M+, there was a decrease in the percentage of Mac+Ia+ M+. Also, TBH splenic M+ showed a smaller but more uniform antigen density than normal host splenic M+. Tumor growth modulated phenotypic alterations in peritoneal and splenic M+ subpopulations. Combined with earlier functional studies of M 4 subpopulations, these data suggested a relationship between changes in M+ phenotype and tumor-induced dysfunction of M+-modulated immune activity.Key terms: Macrophage surface antigens, double-label techniques, monoclonal antibodies, Mac-1, Mac-2, Mac-3, Ia, tumor-bearing host Macrophages (M+) have diverse functions that may reflect the existence of distinct subpopulations of M+ (9-11,16,29-31,341. To understand M+ functions during the immune response, these subpopulations need to be characterized and their functional relationships established. Subpopulation delineation is critical in studies involving tumor-bearing hosts (TBH), because tumor growth changes the immunoregulatory properties of M+ (6)(7)(8)(9)(10)(11)(15)(16)(17)(18)24,25,28,33,35,36) and changes also occur in M+ phenotype and function. Phenotypic changes in TBH M+ include changes in surface antigen (Mac-1, Mac-2, Mac-3, and Ia) expression (9)(10)(11)16,19,25,34), size distribution (341, and peroxidase staining (11). Concomitant functional changes in TBH M+ include decreased accessory cell function in the allogeneic mixed lymphocyte reaction (MLR) (6,10,11,16,24), the autologous mixed lymphocyte reaction (AMLR) (8,35,36), and mitogen-induced T cell proliferation (7,9,10,25,33). These functional changes result from an altered suppressor M+ population (9,11,16,25,(34)(35)(36) and a decreased production of enhancing factors (18,28,35). If the phenotype of the suppressor M+ could be identified (reported here) and then correlated with its specific function, a greater understanding of the mechanism of TBH M+-mediated control of immune reactivity would emerge.

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Section: Dual Antigen Expression On Splenic M+supporting

confidence: 85%

Section: Dual Antigen Expression On Splenic M+mentioning

confidence: 99%

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Two‐color flow cytometric analysis of the expression of MAC and MHC Class II antigens on macrophages during tumor growth

1990

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“…Only recently has the participation of circulating monocytes in experimental glomerulonephritis been delineated (3,22), and the stimulus causing their infiltration remains obscure, except for the finding that it is not complement dependent (27). The (28). They degrade and process antigen to selectively initiate cellular and humoral immune responses by lymphocytes.…”

Section: Resultsmentioning

confidence: 99%

Characterization of resident glomerular cells in the rat expressing Ia determinants and manifesting genetically restricted interactions with lymphocytes.

Schreiner¹,

Kíely²,

Cotran³

et al. 1981

J. Clin. Invest.

164

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“…The existence of Fc receptors of IgG on macrophages has been well documented (Unanue, 1981). It is conceivable that the IgG of an immune serum binds to the surface of the trophozoite by its Fab portion and to the macrophage by its Fc portion, thus signalling the macrophage to kill the trophozoite, It seems that attachment of macrophages to the trophozoite is necessary to induce cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Antibody-Dependent Macrophage-Mediated Cytotoxicity Against Entamoeba Histolytica

Saxena

Chugh

Vinayak³

1986

Journal of Medical Microbiology

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The Regulatory Role of Macrophages in Antigenic Stimulation Part Two: Symbiotic Relationship between Lymphocytes and Macrophages

Cited by 465 publications

References 547 publications

Two‐color flow cytometric analysis of the expression of MAC and MHC Class II antigens on macrophages during tumor growth

Two‐color flow cytometric analysis of the expression of MAC and MHC Class II antigens on macrophages during tumor growth

Characterization of resident glomerular cells in the rat expressing Ia determinants and manifesting genetically restricted interactions with lymphocytes.

Antibody-Dependent Macrophage-Mediated Cytotoxicity Against Entamoeba Histolytica

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