The Relationship Between Endogenous <i>β</i>‐Glucuronidase Activity and Biologically Active Flavones‐Aglycone Contents in Hairy Roots of Baikal Skullcap

Dikaya, Varvara; Solovyeva, Aleksandra I.; Sidorov, Roman A.; Solovyev, Pavel A.; Stepanova, A. Yu.

doi:10.1002/cbdv.201700409

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…It is also interesting that the content of methylated flavones exceeded the content of unmethylated ones in the hairy roots of S. lateriflora . Previously, a similar result was obtained in a study of the hairy roots of S. baicalensis , which were also obtained in our laboratory [ 44 , 45 ]. It was suggested that a significant content of methylated flavones is a feature of the hairy roots of this skullcap species, however, the presence of wogonoside and wogonin in S. przewalskii and S. baicalensis indicated possible changes associated with the insertion of agrobacterial genes during the transformation, which is interesting and requires further study.…”

Section: Resultssupporting

confidence: 84%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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We measured and studied the growth parameters and the qualitative and quantitative composition of the flavones of hairy roots of the Scutellaria genus: S. lateriflora, S. przewalskii and S. pycnoclada. Hairy roots were obtained using wild-type Agrobacterium rhizogenes A4 by co-cultivation of explants (cotyledons) in a suspension of Agrobacterium. The presence of the rol-genes was confirmed by PCR analysis. The hairy roots of the most studied plant from the Scutellaria genus, S. baicalensis, were obtained earlier and used as a reference sample. HPLC-MS showed the predominance of four main flavones (baicalin, baicalein, wogonin and wogonoside) in the methanol extracts of the studied hairy roots. In addition to the four main flavones, the other substances which are typical to the aerial part of plants were found in all the extracts: apigenin, apigetrin, scutellarin and chrysin-7-O-β-d-glucuronide. According to the total content of flavones, the hairy roots of the studied skullcaps form the following series: S. przewalskii (33 mg/g dry weight) > S. baicalensis (17.04 mg/g dry weight) > S. pycnoclada (12.9 mg/g dry weight) > S. lateriflora (4.57 mg/g dry weight). Therefore, the most promising producer of anti-coronavirus flavones is S. przewalskii.

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Section: Resultssupporting

confidence: 84%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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“…When the plant of S. baicalensis is affected by external stress environment, the cells will produce a large amount of H 2 O 2 ( Fu et al, 2018 ). Baicalin, a glycoside component in roots of S. baicalensis , is immediately hydrolyzed to baicalein (an aglycone component) under the action of GUS ( Dikaya et al, 2018 ; Matsuda et al, 2000 ). In this study, we revealed the dynamic change of the effects of storage time on the contents of eight active ingredients in fresh-crushed and fresh-cut roots at room temperature.…”

Section: Discussionmentioning

confidence: 99%

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Bai

Yang

Cao

et al. 2020

Industrial Crops and Products

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“…The phenomenon resulted in a lower baicalin content in the samples that were dried in the shade than for those that were dried in the sun. The possible explanations for this phenomenon are that baicalin is immediately hydrolyzed to baicalein by the action of the endogenous enzyme glucuronidase (GUS), which is active for a more extended period under shade drying conditions [ 41 , 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Post-Harvest Processing Methods Have Critical Roles in the Contents of Active Ingredients of Scutellaria baicalensis Georgi

Zhang

Liang

2022

Molecules

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To find the best post-harvest processing method for Scutellaria baicalensis Georgi, we explored the effects of fresh and traditional processing on the active ingredients in S. baicalensis and evaluated three drying techniques to determine the optimal post-harvest processing technique. We quantified four active ingredients (baicalin, baicalein, wogonoside, and wogonin) in 16 different processed S. baicalensis samples that were harvested from Tongchuan, Shaanxi province, by HPLC (high-performance liquid chromatography). In addition, we performed a similarity analysis (SA), a hierarchical cluster analysis (HCA), and a principal component analysis (PCA) on the common peaks in S. baicalensis that were identified by the HPLC fingerprints. Compared to the traditional processing method, the fresh processing method could better preserve the four active ingredients in S. baicalensis, meanwhile, the similarity analysis (0.997–1.000) showed that the fresh processing was more similar to the traditional processing, and it did not change the type of 18 active ingredients in S. baicalensis. The cluster analysis results showed that the shade drying and sun drying methods results were more similar to each other, while the oven drying (60 °C) method results were clustered into one category. According to the results of the principal component analysis, S9, S7, and S8 had higher scores, and they were relatively well processed under these processing settings. Fresh processing could be an alternative to traditional processing; the moisture content was reduced to 24.38% under the sun drying condition, and it was the optimal post-harvest processing solution for S. baicalensis.

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The Relationship Between Endogenous β‐Glucuronidase Activity and Biologically Active Flavones‐Aglycone Contents in Hairy Roots of Baikal Skullcap

Cited by 9 publications

References 47 publications

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Post-Harvest Processing Methods Have Critical Roles in the Contents of Active Ingredients of Scutellaria baicalensis Georgi

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