The relationship between glucose concentration and rate of lactate production by human erythrocytes in an open perfusion system

Kuchel, Philip W.; Chapman, Bogdan E.; Lovric, V.A.; Raftos, Julia E.; Stewart, I. M.; Thorburn, David R.

doi:10.1016/0167-4889(84)90168-x

Cited by 19 publications

(21 citation statements)

References 23 publications

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“…In addition, using this assumption it was predicted that the concentration of ATP at both pH 6.9 and pH 7.4 would be equal. This is in agreement with the finding of Rapoport et al [43] that the concentration of ATP in erythrocytes incubated with glucose (Glc) at different pH values is constant ; this finding was also reported by Jacobasch et al [35]. Note, however that Minakami and Yoshikawa [37] have reported a pH-dependence of ATP concentration.…”

Section: Phsupporting

confidence: 93%

“…In support of the current model's prediction of [Mg# + ]-sensitivity is the finding that if the concentration of total Mg# + was reduced by a factor of 2 (which corresponds to a decrease in free Mg# + by a factor of $ 3) the model predicts that the glycolytic rate would decrease by $ 30 %. This is in agreement with the decline in the glycolytic rate measured in dog and rat erythrocytes which have had their Mg# + concentrations reduced by a factor of $ 2 [35]. Another interesting observation to emerge from the simulations was that the elasticity of the ATPase to free Mg# + concentration is the parameter most responsible for the response of glycolytic flux to Mg# + at low total concentrations of Mg# + .…”

Section: Total Mg 2 +supporting

confidence: 81%

“…There are a number of experimental difficulties in accurately determining this ratio ; however, its value is probably between 40 and 1000 [35].…”

Section: Nad + /Nadh Ratiomentioning

confidence: 99%

“…This model is a modification of that presented in [35]. Symbols : E, free enzyme ; A, MgATP ; B, Glc ; P, Glc6P ; Q, MgADP ; I 1 , P i ; I 2 , 2,3-BPG ; I 3 , Glc(1,6)P 2 ; I 4 , GSH ; all other species are the corresponding enzyme-metabolite complexes.…”

Section: Scheme A1 a Minimal Model Of Human Erythrocyte Hkmentioning

confidence: 99%

“…(A2), k cat,f (pH l 7.2) was calculated to be 180 s − 1 ; h the concentration of HK in human erythrocytes, e o , was estimated from the measured human erythrocyte activity (182 units:litre of erythrocytes − 1 , 37 mC, pH 7.2 ; [35]) and the value of k cat,f (pH l 7.2).…”

Section: Table A1 a Comparison Of The Reported Kinetic Parameters Of mentioning

confidence: 99%

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Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Mulquiney

Kuchel

1999

Biochemical Journal

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Over the last 25 years, several mathematical models of erythrocyte metabolism have been developed. Although these models have identified the key features in the regulation and control of erythrocyte metabolism, many important aspects remain unexplained. In particular, none of these models have satisfactorily accounted for 2,3-bisphosphoglycerate (2,3-BPG) metabolism. 2,3-BPG is an important modulator of haemoglobin oxygen affinity, and hence an understanding of the regulation of 2,3-BPG concentration is important for understanding blood oxygen transport. A detailed, comprehensive, and hence realistic mathematical model of erythrocyte metabolism is presented that can explain the regulation and control of 2,3-BPG concentration and turnover. The model is restricted to the core metabolic pathways, namely glycolysis, the 2,3-BPG shunt and the pentose phosphate pathway (PPP), and includes membrane transport of metabolites, the binding of metabolites to haemoglobin and Mg# + , as well as pH effects on key enzymic reactions and binding processes. The

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Section: Phsupporting

confidence: 93%

Section: Total Mg 2 +supporting

confidence: 81%

“…There are a number of experimental difficulties in accurately determining this ratio ; however, its value is probably between 40 and 1000 [35].…”

Section: Nad + /Nadh Ratiomentioning

confidence: 99%

Section: Scheme A1 a Minimal Model Of Human Erythrocyte Hkmentioning

confidence: 99%

Section: Table A1 a Comparison Of The Reported Kinetic Parameters Of mentioning

confidence: 99%

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Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Mulquiney

Kuchel

1999

Biochemical Journal

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Diffusion of solutes in agarose and alginate gels: ¹H and ²³Na PFGSE and ²³Na TQF NMR studies

Lundberg

Kuchel

1997

Magnetic Resonance in Med

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Cells immobilized in gels experience potential metabolic restrictions in the form of reduced diffusion rates of metabolites and ions and their possible selective adsorption on the gel matrix. Diffusion and relaxation characteristics of common solutes in agarose and barium alginate gels were investigated at 37 degrees C by using 1H PFGSE and 23Na TQF NMR spectroscopy. Glucose, glycine, alanine, lactate, sodium ions, and HDO were studied. There were no selective interactions between any of the metabolites and the gel materials but the diffusion coefficients were uniformly reduced. The effects of metabolite diffusion and utilization, in gel beads and threads containing cells, were simulated by using a reaction diffusion model incorporating the measured diffusion coefficients. Metabolism is expected to be very significantly limited by diffusion of solutes to and from the cells that are centrally located within gel threads or spheres of radius approximately 2.0 mm, which is a commonly used size.

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NMR Studies of Metabolism of Cells and Perfused Organs

Kaplan

Cohen

1992

In-Vivo Magnetic Resonance Spectroscopy III: In-Vivo MR Spectroscopy: Potential and Limitations

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The relationship between glucose concentration and rate of lactate production by human erythrocytes in an open perfusion system

Cited by 19 publications

References 23 publications

Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Diffusion of solutes in agarose and alginate gels: ¹H and ²³Na PFGSE and ²³Na TQF NMR studies

NMR Studies of Metabolism of Cells and Perfused Organs

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The relationship between glucose concentration and rate of lactate production by human erythrocytes in an open perfusion system

Cited by 19 publications

References 23 publications

Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: equations and parameter refinement

Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies

NMR Studies of Metabolism of Cells and Perfused Organs

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Product

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Diffusion of solutes in agarose and alginate gels: ¹H and ²³Na PFGSE and ²³Na TQF NMR studies