The Relationship between Haptoglobin Polymorphism and Oxidative Stress in Hemodialysis Patients

SummaryBackground: This study was conducted to evaluate the influence of glutathione-S-transferase (GST) M1 and T1 polymorphisms in 184 patients with different stages of liver fibrosis and hepatitis C virus infection and 173 healthy control subjects. Methods: DNA samples were extracted from whole blood, and the polymorphisms of GSTM1 and GSTT1 were determined with PCR using fluorescence-labeled Taq Man probes. Associations between specific genotypes and progression of liver fibrosis were examined by use of the logistic regression analysis to calculate the odds ratio (OR) and 95% confidence intervals (CI). Results: Results show that no differences were found between the frequencies of GSTM1 (49.8% versus 50.2%) and GSTT1 (52.2% versus 47.8%) null genotypes in HCV-infected pa tients and healthy controls, respectively. In addition, there was also no significant relation between the frequency of GSTM1 or GSTT1 gene polymorphisms and fibrosis stage as classified by the METAVIR group. Conclusions: The combined GSTM1 and GSTT1 null genotypes showed an association between GSTM1 [-]/GSTT1 [-] and progression of liver fibrosis.

Section: Introductionmentioning

confidence: 99%

Glutathione-S-Transferase M1 and T1 Gene Polymorphisms and Susceptibility to the Progression of Liver Fibrosis in Hcv-Infected Patients in Taiwan

Sun¹,

Jeng²,

Lee³

et al. 2014

“…Oxidative stress is the condition arising from the imbalance between toxic reactive oxygen species (ROS) and antioxidant systems (5), being also recognized as a very important mechanism in a variety of pathologies (6) including neuropsychiatric disorders, as our group has previously demonstrated (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

Alexinschi¹,

Chiriță²,

Ciobîcă³

et al. 2014

SummaryBackground: Although it is generally accepted that there is an increased oxidative stress status in alcoholics, the separate relevance of oxidative stress following alcohol withdrawal is still not understood to this date. There are reports stating that the increased oxidative stress status in alcoholics may persist independently of the constant presence of alcohol intake, while on the other side, it was demonstrated that the antioxidant defense mechanism could significantly increase after alcohol withdrawal. Methods: In the present work, we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers (two antioxidant enzymes: superoxide dismutase -SOD and glutathione peroxidase -GPX and a lipid peroxidation maker -MDA) after one week and one month of abstinence, as compared to the baseline and a control group of subjects. Results: Our data confirmed the increased oxidative stress status in alcoholic patients and, more importantly, we de monstrated here a significant decrease of the oxidative stress status one week and one month following the withdrawal, as showed by a significant increase in the specific activity of

“…AOPP are also recognized as markers of inflammation, with high levels being reported in diseases such as diabetes, chronic renal failure, hyperlipidemia, etc. (29,30). Neutrophil oxidative potential was shown to be directly involved in plasma AOPP formation through the activity of myeloperoxidase, that is mostly abundant in these cells (8,27,28).…”

Section: Discussionmentioning

confidence: 99%

Lipid Peroxidation and Oxidative Protein Products in Children With Episodic Fever of Unknown Origin / Lipidna Peroksidacija I Oksidativni Proteinski Produkti Kod Dece Sa Epizodinom Groznicom Nepoznatog Uzroka

Radović¹,

Vojinović²,

Bojanić³

et al. 2013

SummaryBackground: Episodic fever syndromes are commonly seen in pediatric practice. Episodic fever of unknown origin (FUO) lasts for a few days or weeks and is followed by a fever-free period with a sense of well-being. In this condition, activated neutrophils and monocytes intensively generate reactive oxidative species that may further damage various molecules. The aim of the study was to evaluate oxidative stress biomarkers, lipid peroxidation in erythrocytes and plasma, and advanced oxidation protein products (AOPP) in children with episodic FUO. Methods: The study enrolled 25 children with episodic FUO in afebrile phase and 25 healthy children as controls. Lipid peroxidation was evaluated by measuring malondialdehyde (MDA) production with the thiobarbituric-acid-reactive substances (TBARS) assay in erythrocytes and plasma. Oxidative modification of proteins was measured spectrophotometrically by the determination of AOPP in plasma. Results: Mean duration of episodic fevers was 3.96±2.8 years. Erythrocyte MDA levels were higher in children with FUO than in controls (86.26±10.75 vs. 78.0±3.21 nmol/g hemoglobin), although not significantly (p=0.202). The MDA plasma concentrations were similar (2.42±0.35 vs. 2.41±0.39 mmol/L) between the groups (p=0.732). Unexpectedly, levels of AOPP were significantly lower in children with FUO than in healthy controls (18.8±5.04 vs. 25.1±3.35 mmol/L, p=0.047).