The Northeast region of Brazil (NEB) has a high rate of deaths from lightning strikes (18% of the country’s total). The region has states, such as Piauí, with high mortality rates (1.8 deaths per million), much higher than the national rate (0.8) and the NEB rate (0.5). In this sense, the present work analyzes the microphysical characteristics of clouds with and without the occurrence of total lightning. For this purpose, data from the Lightning Imaging Sensor (LIS), TRMM Microwave Imager (TMI) and Precipitation Radar (PR), aboard the Tropical Rainfall Measuring Mission (TRMM) satellite from 1998 to 2013 were used. The TRMM data were analyzed to establish a relationship between the occurrence of lightning and the clouds’ microphysical characteristics, comparing them as a function of lightning occurrence classes, spatial location and atmospheric profiles. A higher lightning occurrence is associated with higher values of ice water path (>38.9 kg m−2), rain water path (>2 kg m−2), convective precipitation (>5 mm h−1) and surface precipitation (>7 mm h−1), in addition to slightly higher freezing level height values. Reflectivity observations (>36 dBZ) demonstrated typical convective profile curves, with higher values associated with classes with higher lightning densities (class with more than 6.8 flash km−2 year−1).