THE RELATIONSHIP BETWEEN MALE STERILITY AND MEMBRANE LIPID PEROXIDATION AND ANTIOXIDANT ENZYMES IN WHEAT (Triticum aestivum L.)

Abstract

Malondialdehyde (MDA), which is thought to be important inducible factor of cell apoptosis if excessively accumulated in cells, is often regarded as a widely used marker of oxidative lipid injury whose concentration varies in response to biotic and abiotic stress. To elucidate the metabolic mechanism of ROS production and scavenging of the male sterility, normal fertility and male sterility wheat, including genetic and physiologically male sterility, by comparing MDA content and antioxidant enzymes changes in the sterile and fertile leaves, spikes, florets, rachillaes, ovaries and anthers at different developmental anther stages, were employed for studying the relationship between the male sterility and membrane lipid peroxidation, antioxidant enzymes. The results showed that tissue of male sterility had roughly higher content of MDA than corresponding fertile plants, and ovary performance was not obvious but most obvious in anther. Simultaneously, the higher activities of antioxidant enzymes were measured in the anthers of male sterility plant, in which an increase of ROS in anthers of abortion stage had an inducible effect on the antioxidant enzymes. MDA content and scavenging-enzyme activities had difference in the sterile and fertile leaves, spikes, florets, rachillaes at different anther stages, indicating that different tissues of ROS metabolism had noticeable difference in male sterility and normal wheat. It can be considered that the disorder of scavenging-enzyme activities and accumulation of MDA in the male organ, and supplier of nutrition blades were causing a further substance metabolism disorder, energy loss, leading to the occurrence of male sterility.

The Comparison of the Chemical Composition, Sensory, Phenolic and Antioxidant Properties of Juices from Different Wheatgrass and Turfgrass Species

“…There are several factors, such as genetics, plant species, agronomic and environmental conditions, pH, light intensity, type of light, low and high temperatures, and minerals absorbed by the plant via roots, that affect different properties of the wheatgrass such as plant colour, oil, dry matter, °Brix, protein, total phenols, total flavonoids, vitamin C, DPPH, and macro element contents and radical scavenging activity Devi Sowjanya et al, 2015;Khampas et al, 2015;Liu et al, 2015;Rimple et al, 2016).…”

Evaluation of Diet Supplementation with Wheat Grass Juice on Growth Performance, Body Composition and Blood Biochemical Profile of Carp (Cyprinus carpio L.)

“…Another class of bioactive compounds found in WGJ are total phenols and total flavonoids (164 µg mL −1 and 289 µg mL −1 , respectively), although the values are lower than in [ 29 ] (342 mg L −1 and 598 mg L −1 , respectively) the antioxidant activity was high (67% DPPH% inhibition). Variation in biochemical composition of plant extracts is normal and is dependent on plant species and cultivar, cultivation conditions, extraction techniques and methods of investigation [ 34 ] These compounds are widely used in medicine due to their health related benefits, such as prevention or reduction of heart disease and diabetes, as well as antibacterial, antiviral, anti-inflammatory, and anti-allergenic properties [ 35 , 36 ].…”

Analysis of chalcone synthase gene in chemically induced male sterility (CIMS) in wheat (Triticum aestivum L.)

“…Pollen grains were collected, stained with a 1% KI-I2 solution, and observed under a fluorescence microscope. Spherical and dark pollen grains were considered healthy and fertile, whereas shrunken and unstained pollen grains were regarded as sterile (Liu et al 2015).…”