The Relationship between p53-Positive Neurons and Dark Neurons in the Hippocampus of Rats after Surgical Interventions on the Nasal Septum

Торшин, В. И.; Кастыро, И. В.; Reshetov, Igor V.; Kostyaeva, M. G.; Popadyuk, V. I.

doi:10.1134/s1607672922010094

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…Thus, in fibromyalgia, an increase in LF/HF corresponds to a shift in the ANS to the dominance of the sympathetic division or to a decrease in the role of the parasympathetic, which is quite consistent with the nature of the autonomic function in fibromyalgia [22]. Septoplasty, in comparison with other models of operations in the maxillofacial region, in itself provokes an increase in the vagosympathetic index, which is due to the large area of the surgical field, a decrease in nasal passages, and sensory deprivation of the olfactory analyzer [1,2,7]. In the bulbectomy group, this figure is even higher, which can be explained by the complete absence of the function of the olfactory analyzer [8].…”

Section: Discussionmentioning

confidence: 65%

“…Previously, it was shown that modeling septoplasty in rats provokes the appearance of an anxietydepressive state, which is manifested by changes in the behavior of animals [8,23]. It is also known that surgical alteration in the maxillofacial region leads to changes in the cytoarchitectonics of the hippocampal pyramidal layer, an increase in neuronal apoptosis in the ammonium horn of the hippocampus [2], pronounced local inflammatory reactions of the depressive state, which is manifested by changes in the behavior of animals [15]. In addition, modeling of septoplasty in the early postoperative period provokes an increase in the activity of the sympathetic nervous system [8,23], which is consistent with the data obtained in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Modeling of septoplasty (Group 1, n=10) was performed by the standard method by zigzag scarification of the nasal mucosa (Fig. 1a) [1,2,7]. Bulbectomy.…”

Section: Methodsmentioning

confidence: 99%

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Modeling Septoplasty and Sensory Deprivation in Rat Olfactory Analyzer and Its Impact on the Responses of the Autonomic Nervous System

Mnatsakanyan¹,

Korolev²,

Иноземцев³

et al. 2023

ArveMED

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

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Modeling Septoplasty and Sensory Deprivation in Rat Olfactory Analyzer and Its Impact on the Responses of the Autonomic Nervous System

Mnatsakanyan¹,

Korolev²,

Иноземцев³

et al. 2023

ArveMED

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“…Morphological changes in the hippocamp are confirmed by our previous studies, which showed that septoplasty simulation in rats provokes the development of many stress reactions [34] and even a breakdown in adaptation [35]: an increase in the number of dark neurons [36], the expression of the p53 protein [3], a significant release of corticosterone into the blood plasma [37], and an increase in degranulation of mast cells [38], disturbances in the balance of the autonomic nervous system [37,39,40], changes in behavior when testing rats in an open field [9], the appearance of anxiety and a depressive-like state [37,[41][42][43].…”

Section: Discussionmentioning

confidence: 99%

Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Кастыро

Mikhalskaia

Khamidulin

et al. 2023

Cell Physiol Biochem

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Background/Aims: to determine the role of surgical stress on the formation of p53-positive and dark neurons (DN) in the hippocampus, and to examine the parallelism of their formation in the pyramidal layer of the hippocampus. Methods: Simulated septoplasty was performed on 20 Wistar rats. The hippocampus and dentate gyrus (DG) were examined, in which the number of DN and p53-positive neurons was determined at 2, 4 and 6 days after surgery. In each rat, 10 brain slices were stained with antibodies to p53 protein with Meyer’s haematoxylin and 10 slices were stained with Nissl toluidine blue. Hippocampal subfields CA1, CA2, CA3 and DG were studied. In the pyramidal subfield layer, the absolute number of neurons that were nuclear antibody-positive to p53 protein was counted, as well as the number of dark neurons. The counting area in each subfield was 20934±1260 µm2. Neurons are counted using the Aperio ImageScope program. For the histological specimen analysis, the ImageJ software was used. The data obtained using cell counting methods were presented as mean ± SE. Then, they were compared between both groups using a t-test SPSS 21software. Results: Compared with the control group (n = 5), the number of DN and p53-positive neurons increased in experimental animals at all periods. A direct relationship was obtained between the increase in the number of DN and p53-positive neurons in the hippocampal formation. Septoplasty simulation in rats results in the pathogenetic cascades onset, which, in its turn, changes the morpho-functional properties of neurons of the pyramidal layer of the hippocampus and contributes to their neuroplasticity. Activation of NMDS receptors of neurons during stress apparently, initiates two ways of neuron life – the beginning of p53 protein expression and the DN formation. Both ways can finally lead to apoptosis. Conclusion: The formation of dark neurons and the expression of the p53 protein in them are most likely to be interconnected and can probably provide neuroprotective mechanisms.

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“…Важную роль в развитии и функционировании головного мозга играют нейральные стволовые клетки (NSC). В зубчатой извилине гиппокампа взрослых млекопитающих непрерывно рождаются новые нейроны [54]. В возрасте 2 мес зубчатая извилина взрослых мышей производит в среднем примерно один новый нейрон гранулярных клеток на 1700 существующих нейрональных гранулярных клеток в день [55].…”

Section: роль белка р53 в функционировании нервной системыunclassified

Regulatory role of protein p53 in the functional activity of the central nervous system

Kotov,

Kostyaeva,

Ibadullaeva

et al. 2024

Morphology

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p53 is one of the most popular research objects among scientists. Over the past 40 years since its discovery, more than 100 thousand scientific works have been written and their number continues to grow steadily. The increased interest in this protein among doctors is due to the participation of p53 in the development of malignant tumors - a socially significant group of diseases of the 21st century. p53 is a tumor suppressor. Normally, when exposed to damaging factors, this protein promotes DNA repair or apoptosis, depending on the damage, which in turn prevents the accumulation of cells with mutant DNA. When p53 mutates, it loses its function, leading to abnormal cell proliferation and tumor progression. However, the role of p53 is not limited to participation in carcinogenesis. Another, no less important and interesting function is the participation of this protein in the regulation of the activity of the central nervous system, but its role is ambiguous. On the one hand, p53 is involved in the embryogenesis of nervous tissue and promotes the differentiation of neural stem cells; on the other hand, p53 can also have a damaging effect on neurons. The literature review presents current data on the structure and function of the main regulator of the human genome, the p53 protein, and its homologues p63 and p73. The participation of these proteins in programmed cell death and carcinogenesis is considered. Special attention is paid to the role of proteins of the p 53 family in the functioning of cells of the central nervous system and neuroprotection.

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The Relationship between p53-Positive Neurons and Dark Neurons in the Hippocampus of Rats after Surgical Interventions on the Nasal Septum

Cited by 12 publications

References 15 publications

Modeling Septoplasty and Sensory Deprivation in Rat Olfactory Analyzer and Its Impact on the Responses of the Autonomic Nervous System

Modeling Septoplasty and Sensory Deprivation in Rat Olfactory Analyzer and Its Impact on the Responses of the Autonomic Nervous System

Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Regulatory role of protein p53 in the functional activity of the central nervous system

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