The relationship between plasma free 15-F2t–isoprostane concentration and early postoperative cardiac depression following warm heart surgery

Pathophysiology of isoprostanes in the cardiovascular system: implications of isoprostane‐mediated thromboxane A2 receptor activation

“…In a canine model of coronary thrombolysis and in patients with acute myocardial infarction, an increase in urinary 8-iso-PGF2α concentrations of approximately 28 and 300%, respectively, was observed, indicating that coronary reperfusion is associated with an increased generation of isoprostanes, which is likely to reflect oxidant stress in vivo . Furthermore, in patients undergoing coronary artery bypass surgery or acute revascularization in the context of myocardial infarction, a 2-3-fold increase in plasma and urinary 8-iso-PGF2α levels has been detected, confirming the association between ischaemia/reperfusion and isoprostane generation Ansley et al, 2003). The generation of isoprostanes resulting from lipid peroxidation seems to occur immediately after reperfusion because no further increase in the isoprostane concentration could be observed in subsequent post-operative period Ulus et al, 2003).…”

N-acetylcysteine attenuates PKCβ2overexpression and myocardial hypertrophy in streptozotocin-induced diabetic rats

“…Plasma samples or homogenized heart tissue (in PBS) were purified using Affinity Sorbent/Column (Cayman chemical) in the presence of 0.01% butylated hydroxytoluene (BHT) and then processed for analysis of free 15-F 2t -IsoP as previously described [18,19].…”

Bosentan Affects 15-F2t-isoprostane Adverse Effects on Postischemic Rat Hearts

“…15-F 2t -isoprostane-stimulated ET-1 release during early reperfusion seems to be beneficial in facilitating functional recovery in the damaged myocardium, while increased ET-1 production during later reperfusion is detrimental to the heart. The clinical implication in a setting like cardiac surgery associated with myocardial reperfusion injury and oxidative stress [4,40] is that application of ET-1 antagonists during the early phase of reperfusion may have an adverse effect on cardiac function, creating the need for increased postoperative hemodynamic support. This might be especially pertinent in patients with already compromised preoperative cardiac function.…”

“…15-F 2t -isoprostane (old name: 8-isoprostane or 8-iso-prostaglandin F2a) [3], particularly, has been found to be a specific, reliable marker of reactive oxygen species (ROS)-induced oxidative stress. Its formation is increased after myocardial ischemia and during reperfusion in patients undergoing coronary artery bypass graft surgery using cardiopulmonary bypass [4]. Also, 15-F 2t -isoprostane has recently been identified as a sensitive and independent risk marker of coronary artery disease [5], and urinary levels of 15-F 2t -isoprostane can indicate the presence, severity, and extent of angiographic coronary artery disease [6].…”