The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes

Otsuki, Junko; Okada, Akiko; Morimoto, Kazushige; Nagai, Yasushi; Kubo, Harumi

doi:10.1093/humrep/deh258

Cited by 171 publications

(181 citation statements)

References 33 publications

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“…1a). The presence of vacuoles or inclusions within the cytoplasm was also considered because the presence of these dysmorphisms is considered an abnormality [42][43][44]49]. Basically, oocytes lacking both vacuoles and inclusions were awarded three points, oocytes with either small amounts of vacuoles or inclusions two points, and oocytes containing both vacuoles and inclusions one point (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Human metaphase II oocytes have distinct morphological characteristics that may be indicative of quality and therefore used to predict implantation potential [21,[36][37][38][39][40][41][42][43]. For example, regular patterns of granularity have been observed in the oocyte cytoplasm and it has been suggested that these patterns correlate with oocyte quality [44,45].…”

Section: Introductionmentioning

confidence: 99%

“…For example, regular patterns of granularity have been observed in the oocyte cytoplasm and it has been suggested that these patterns correlate with oocyte quality [44,45]. Human oocytes may contain inclusions or vacuoles which may be detrimental to the implantation potential of this material [37,38,[41][42][43]. The oocyte plasma membrane response to penetration with an ICSI pipette has also been suggested to be a useful determinant of oocyte quality [46].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

J Assist Reprod Genet

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Purpose In this work, we describe a system for the morphological scoring of human oocytes prior to fertilisation and use this system to test whether oocyte morphology is an indicator of fertilisation, embryo development and implantation potential. Methods The study is a prospective trial of the use of oocyte morphological scores in 822 patients undergoing their first cycle of ICSI. Analyses of oocytes were performed prior to ICSI procedures and the scores compared with fertilisation rates, embryo quality and clinical results.Results 'Top quality' oocytes had a significantly higher level of fertilisation (96%) as compared to low scoring oocytes (25.6%). Where top quality oocytes formed top quality embryos, we noted a clinical success rate of 63.4%. Conclusions Clinical success rates were increased in cases where top quality oocytes formed top quality embryos after ICSI. The analysis of oocyte morphology may represent a positive selection feature during ICSI.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

J Assist Reprod Genet

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show abstract

“…Nonetheless, Otsuki et al [26] demonstrated that the presence of SER is associated with lower chances of successful pregnancy and even if normal oocytes from the same IU international units cohort are transferred, pregnancy rates are compromised. Kahraman et al [27] showed that, despite normal pregnancy rates were obtained using oocytes with centrally located granular cytoplasm, more than 50% of pregnancies resulted in abortions and implantation rate was as low as 5%.…”

Section: Discussionmentioning

confidence: 99%

Oocyte morphology does not affect post-warming survival rate in an egg-cryobanking donation program

Setti

Figueira²,

Braga

et al. 2011

J Assist Reprod Genet

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Purpose To evaluate whether oocyte dysmorphisms affect oocyte survival rates in an egg-cryobanking donation program. Methods This study included 54 patients undergoing intracytoplasmic sperm injection. A total of 415 metaphase II oocytes were vitrified using the Cryotop method. Oocyte morphology was assessed immediately prior to oocyte vitrification under 400× magnification. The influence of dysmorphisms on post-thaw survival rates was assessed using regression analysis. Results were considered to be significant at the 5% critical level. Results Oocyte survival rate was not affected by the presence of the following analysed oocyte abnormalities: increased cytoplasmic granularity, vacuoles in the ooplasm, aggregates of smooth endoplasmic reticulum in the ooplasm, large perivitelline space size, perivitelline space granularity, fragmented first polar body and zona pellucida abnormalities. Conclusions Oocyte morphology, observed prior to vitrification, does not predict post-warming survival. The noninvasive identification of predictive markers for oocyte survival potential remains a difficult task.

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“…Dysmorphic oocytes, i.e., those exhibiting centrally located granular cytoplasm (CLGC) [7], vacuolization [8], or smooth endoplasmic reticulum clusters (sERC) [9,10], are frequently observed [11]. During piezo-ICSI, low-stretching oocytes, which penetrate before piezo pulse application, are also common [12].…”

Section: Introductionmentioning

confidence: 99%

Oocyte quality improvement using a herbal medicine comprising 7 crude drugs

Inoue¹,

Ono²,

Yonezawa³

et al. 2013

OJOG

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Maternal age is a significant factor in infertility treatment. Ovarian function and oocyte quality decrease with age, whereas the frequency of chromosomal abnormalities increases. In this study, improvement of oocyte quality and ovarian function were attempted using a herbal medicine comprising 7 crude drugs: Angelicae radix, Rehmanniae radix, Plantaginis semen, Lonicerae flos, Carthami flos, Ginseng radix, and Cucurbita moschata Duch. Thirty-one women who repeatedly failed to conceive by intracytoplasmic sperm injection took the herbal medicine before breakfast and dinner from the start of menstrual cycle in the ovum pickup cycle. Average patient age was 38.5 ± 0.7 years, and the average ovum pickup frequency on the first dosage day was 7.9 ± 1.5. To analyze the effects of herbal medicine intake, the number of recovered and mature oocytes, their morphology and physical qualities, as well as the rates of fertilization, oocyte development, and pregnancy was compared before and after intake. The recovered and mature oocyte numbers, oocyte morphology and physical qualities, and fertilization rate were not significantly different before and after drug intake. However, the oocyte development rate was significantly higher (58.0%) after herbal medicine intake than before (32.5%; p = 0.0003). Moreover, the successful pregnancy rate was significantly higher after intake than before (6.9% versus 0%; p = 0.0111). Herbal medicine may constitute a useful adjunct to assisted reproductive technology in women.

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The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes

Abstract: The presence of sERCs is associated with lower chances of successful pregnancy, even in sERC-negative oocytes from the same cohort that are transferred along with the sERC-positive oocytes. High estradiol levels could be one of the causes of sERC formation.

Cited by 171 publications

References 33 publications

An oocyte score for use in assisted reproduction

An oocyte score for use in assisted reproduction

Oocyte morphology does not affect post-warming survival rate in an egg-cryobanking donation program

Oocyte quality improvement using a herbal medicine comprising 7 crude drugs

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